Application of marker parameters of roach (Rutilus rutilus Linnaeus, 1758) for diagnosis of ecological condition of water bodies in conditions of excessive anthropogenic pollution
Purpose. To investigate changes in the biochemical parameters of roach as a species response to the effects of anthropogenic pollution, which is necessary for further biomarking of the aquatic environment. Methodology. Fish were caught using hook and line fishing gears. Following biochemical para...
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| Format: | Article |
| Language: | English |
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Institute of Fisheries
2020-09-01
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| Series: | Ribogospodarsʹka Nauka Ukraïni |
| Subjects: | |
| Online Access: | http://fsu.ua/index.php/en/2020/3-2020-53/2020-03-080-091-kovalenko-ukr |
| _version_ | 1811332935586938880 |
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| author | Yu. Kovalenko A. Potrokhov M. Prychepa L. Gorbatyuk |
| author_facet | Yu. Kovalenko A. Potrokhov M. Prychepa L. Gorbatyuk |
| author_sort | Yu. Kovalenko |
| collection | DOAJ |
| description | Purpose. To investigate changes in the biochemical parameters of roach as a species response to the effects of anthropogenic pollution, which is necessary for further biomarking of the aquatic environment.
Methodology. Fish were caught using hook and line fishing gears. Following biochemical parameters of roach were determined: glycogen and lipid contents, activities of lactate dehydrognase, succinate dehydrogenase, ATPase and alkaline phosphatase.
Findings. Changes in some biochemical parameters of roach under the effect of anthropogenic pollution have been found. A lower content of glycogen and lipids (by 68.65 and 16.12%) was detected in fish from a polluted water body (Lake Kyrylivske) compared to fish from the conditional control (Lake Babyne). The active use of reserved lipids and glycogen emphasizes the general deterioration of ecological conditions in the Lake Kyrylivske.
A higher LDH activity in liver and gill tissues (by 25 and 48%) relative to control was found. In contrast, a lower LDH activity (by 11 and 16%) in all studied tissues (muscles, liver and gills) of roach from the polluted water body was detected. Under conditions of pollution of the water body with toxicants of various chemical nature, aerobic redox processes in roach body are intensified as a result of counteracting a potentially aggressive environment. This is confirmed by a higher LDH activity in liver and gill tissues. According to the results of study, a higher alkaline phosphatase activity was also detected in fish from the Lake Kyrylivske (by 39.9 and 24.6%) compared to the control. This indicates an increase in phosphorylation in these tissues, the process of which is aimed at neutralization and excretion of toxic compounds from the body after their detoxification.
The differences between the studied groups of fish in biochemical parameters prove that they live in significantly different ecological and toxicological conditions.
Originality. For the first time, the results of changes in biochemical markers of roach enzyme activity from some water body of Kyiv, which differ in the level of anthropogenic pollution, are presented.
Practical value. The obtained results of the physiological state of roach can be used as a criterion for assessing the ecological status of individual waterbodies, in particular those that are located in urban areas. |
| first_indexed | 2024-04-13T16:44:33Z |
| format | Article |
| id | doaj.art-426104c82ed2446a9898b32b2ab3b8da |
| institution | Directory Open Access Journal |
| issn | 2075-1508 2312-9581 |
| language | English |
| last_indexed | 2024-04-13T16:44:33Z |
| publishDate | 2020-09-01 |
| publisher | Institute of Fisheries |
| record_format | Article |
| series | Ribogospodarsʹka Nauka Ukraïni |
| spelling | doaj.art-426104c82ed2446a9898b32b2ab3b8da2022-12-22T02:39:08ZengInstitute of FisheriesRibogospodarsʹka Nauka Ukraïni2075-15082312-95812020-09-013(53)809110.15407/fsu2020.03.080Application of marker parameters of roach (Rutilus rutilus Linnaeus, 1758) for diagnosis of ecological condition of water bodies in conditions of excessive anthropogenic pollutionYu. Kovalenko0A. Potrokhov1M. Prychepa2L. Gorbatyuk3Institute of Hydrobiology NANU, KyivInstitute of Hydrobiology NANU, KyivInstitute of Hydrobiology NANU, KyivInstitute of Hydrobiology NANU, KyivPurpose. To investigate changes in the biochemical parameters of roach as a species response to the effects of anthropogenic pollution, which is necessary for further biomarking of the aquatic environment. Methodology. Fish were caught using hook and line fishing gears. Following biochemical parameters of roach were determined: glycogen and lipid contents, activities of lactate dehydrognase, succinate dehydrogenase, ATPase and alkaline phosphatase. Findings. Changes in some biochemical parameters of roach under the effect of anthropogenic pollution have been found. A lower content of glycogen and lipids (by 68.65 and 16.12%) was detected in fish from a polluted water body (Lake Kyrylivske) compared to fish from the conditional control (Lake Babyne). The active use of reserved lipids and glycogen emphasizes the general deterioration of ecological conditions in the Lake Kyrylivske. A higher LDH activity in liver and gill tissues (by 25 and 48%) relative to control was found. In contrast, a lower LDH activity (by 11 and 16%) in all studied tissues (muscles, liver and gills) of roach from the polluted water body was detected. Under conditions of pollution of the water body with toxicants of various chemical nature, aerobic redox processes in roach body are intensified as a result of counteracting a potentially aggressive environment. This is confirmed by a higher LDH activity in liver and gill tissues. According to the results of study, a higher alkaline phosphatase activity was also detected in fish from the Lake Kyrylivske (by 39.9 and 24.6%) compared to the control. This indicates an increase in phosphorylation in these tissues, the process of which is aimed at neutralization and excretion of toxic compounds from the body after their detoxification. The differences between the studied groups of fish in biochemical parameters prove that they live in significantly different ecological and toxicological conditions. Originality. For the first time, the results of changes in biochemical markers of roach enzyme activity from some water body of Kyiv, which differ in the level of anthropogenic pollution, are presented. Practical value. The obtained results of the physiological state of roach can be used as a criterion for assessing the ecological status of individual waterbodies, in particular those that are located in urban areas.http://fsu.ua/index.php/en/2020/3-2020-53/2020-03-080-091-kovalenko-ukrroachanthropogenic pollutionenzyme activityecological condition |
| spellingShingle | Yu. Kovalenko A. Potrokhov M. Prychepa L. Gorbatyuk Application of marker parameters of roach (Rutilus rutilus Linnaeus, 1758) for diagnosis of ecological condition of water bodies in conditions of excessive anthropogenic pollution Ribogospodarsʹka Nauka Ukraïni roach anthropogenic pollution enzyme activity ecological condition |
| title | Application of marker parameters of roach (Rutilus rutilus Linnaeus, 1758) for diagnosis of ecological condition of water bodies in conditions of excessive anthropogenic pollution |
| title_full | Application of marker parameters of roach (Rutilus rutilus Linnaeus, 1758) for diagnosis of ecological condition of water bodies in conditions of excessive anthropogenic pollution |
| title_fullStr | Application of marker parameters of roach (Rutilus rutilus Linnaeus, 1758) for diagnosis of ecological condition of water bodies in conditions of excessive anthropogenic pollution |
| title_full_unstemmed | Application of marker parameters of roach (Rutilus rutilus Linnaeus, 1758) for diagnosis of ecological condition of water bodies in conditions of excessive anthropogenic pollution |
| title_short | Application of marker parameters of roach (Rutilus rutilus Linnaeus, 1758) for diagnosis of ecological condition of water bodies in conditions of excessive anthropogenic pollution |
| title_sort | application of marker parameters of roach rutilus rutilus linnaeus 1758 for diagnosis of ecological condition of water bodies in conditions of excessive anthropogenic pollution |
| topic | roach anthropogenic pollution enzyme activity ecological condition |
| url | http://fsu.ua/index.php/en/2020/3-2020-53/2020-03-080-091-kovalenko-ukr |
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