The effects of severe hypoxia on nitric oxide parameters in hypoxia-tolerant rodent: nannospalax nehringi
Blind mole rats (BMRs) are solitary rodents which are tolerant to severe hypoxia. The aim of this study is to reveal the changes in nitric oxide (NO) and nitric oxide enzymes (NOS), which are involved in many physiological and pathological processes related with hypoxia, in BMRs under severe hypoxia...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Kafkas University, Faculty of Veterinary Medicine
2018-12-01
|
| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=2457 |
| _version_ | 1797800633031458816 |
|---|---|
| author | Barış YILDIZ Nadide Nabil KAMİLOĞLU Metin ÖĞÜN Cem ÖZİÇ Oğuz MERHAN Tarık MECİT Yüksel COŞKUN |
| author_facet | Barış YILDIZ Nadide Nabil KAMİLOĞLU Metin ÖĞÜN Cem ÖZİÇ Oğuz MERHAN Tarık MECİT Yüksel COŞKUN |
| author_sort | Barış YILDIZ |
| collection | DOAJ |
| description | Blind mole rats (BMRs) are solitary rodents which are tolerant to severe hypoxia. The aim of this study is to reveal the changes in nitric
oxide (NO) and nitric oxide enzymes (NOS), which are involved in many physiological and pathological processes related with hypoxia,
in BMRs under severe hypoxia. For this purpose, 12 subadult (11-15 moths) male Nannospalax nehringi were captured in Kars location.
Captured BMRs were divided into two groups as Normoxic (NG) and Hypoxic (HG) randomly (n=6). NG were kept in completely dark,
normoxic conditions for 52 h. HG were kept inside completely dark glovebox chamber with 7% oxygen flow for 52 h. After experimental
protocol, NG were sacrificed under normoxic conditions and HG were sacrificed inside glovebox chamber with 7% oxygen. NO, iNOS,
eNOS, nNOS and MDA levels of plasm and homogenized tissue samples were detected spectrophotometrically. All parameters of each
sample were found to be high in HG compared to NG. But especially, NO was high in the lung tissues of HG. Additionally eNOS level of
the kidney, liver and lung, iNOS levels of the liver and eNOS, and nNOS levels of the brain were found to be markedly high. Consequently,
our data on NO and NOS enzyme production in Nannospalax nehringi tissues under hypoxia are compatible with the data obtained from
other animals, but it contains differences in some points. We believe that these differences are different evolutionary adaptations of BMRs
to hypoxia. |
| first_indexed | 2024-03-13T04:38:11Z |
| format | Article |
| id | doaj.art-4264d74b16bb49658c51ca7105a61023 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:38:11Z |
| publishDate | 2018-12-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-4264d74b16bb49658c51ca7105a610232023-06-19T06:34:42ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512018-12-0125220921410.9775/kvfd.2018.206992457The effects of severe hypoxia on nitric oxide parameters in hypoxia-tolerant rodent: nannospalax nehringiBarış YILDIZ0Nadide Nabil KAMİLOĞLU1Metin ÖĞÜN2Cem ÖZİÇ3Oğuz MERHAN4Tarık MECİT5Yüksel COŞKUN6Department of of Physiology, Faculty of Veterinary Medicine, Kafkas University, TR-36300 Kars - TURKEYDepartment of of Physiology, Faculty of Veterinary Medicine, Kafkas University, TR-36300 Kars - TURKEYDepartment of Biochemistry, Faculty of Veterinary Medicine, Kafkas University, TR-36300 Kars - TURKEYDepartment of Medical Biology, Faculty of Medicine, Kafkas University, TR-36300 Kars - TURKEYDepartment of Biochemistry, Faculty of Veterinary Medicine, Kafkas University, TR-36300 Kars - TURKEYDepartment of of Physiology, Faculty of Veterinary Medicine, Kafkas University, TR-36300 Kars - TURKEYDepartment of Biology, Faculty of Science, Dicle University, TR- 21280 Diyarbakır - TURKEYBlind mole rats (BMRs) are solitary rodents which are tolerant to severe hypoxia. The aim of this study is to reveal the changes in nitric oxide (NO) and nitric oxide enzymes (NOS), which are involved in many physiological and pathological processes related with hypoxia, in BMRs under severe hypoxia. For this purpose, 12 subadult (11-15 moths) male Nannospalax nehringi were captured in Kars location. Captured BMRs were divided into two groups as Normoxic (NG) and Hypoxic (HG) randomly (n=6). NG were kept in completely dark, normoxic conditions for 52 h. HG were kept inside completely dark glovebox chamber with 7% oxygen flow for 52 h. After experimental protocol, NG were sacrificed under normoxic conditions and HG were sacrificed inside glovebox chamber with 7% oxygen. NO, iNOS, eNOS, nNOS and MDA levels of plasm and homogenized tissue samples were detected spectrophotometrically. All parameters of each sample were found to be high in HG compared to NG. But especially, NO was high in the lung tissues of HG. Additionally eNOS level of the kidney, liver and lung, iNOS levels of the liver and eNOS, and nNOS levels of the brain were found to be markedly high. Consequently, our data on NO and NOS enzyme production in Nannospalax nehringi tissues under hypoxia are compatible with the data obtained from other animals, but it contains differences in some points. We believe that these differences are different evolutionary adaptations of BMRs to hypoxia.https://vetdergikafkas.org/pdf.php?id=2457hypoxianoinosenosnnosmdanannospalax nehringi |
| spellingShingle | Barış YILDIZ Nadide Nabil KAMİLOĞLU Metin ÖĞÜN Cem ÖZİÇ Oğuz MERHAN Tarık MECİT Yüksel COŞKUN The effects of severe hypoxia on nitric oxide parameters in hypoxia-tolerant rodent: nannospalax nehringi Kafkas Universitesi Veteriner Fakültesi Dergisi hypoxia no inos enos nnos mda nannospalax nehringi |
| title | The effects of severe hypoxia on nitric oxide parameters in hypoxia-tolerant rodent: nannospalax nehringi |
| title_full | The effects of severe hypoxia on nitric oxide parameters in hypoxia-tolerant rodent: nannospalax nehringi |
| title_fullStr | The effects of severe hypoxia on nitric oxide parameters in hypoxia-tolerant rodent: nannospalax nehringi |
| title_full_unstemmed | The effects of severe hypoxia on nitric oxide parameters in hypoxia-tolerant rodent: nannospalax nehringi |
| title_short | The effects of severe hypoxia on nitric oxide parameters in hypoxia-tolerant rodent: nannospalax nehringi |
| title_sort | effects of severe hypoxia on nitric oxide parameters in hypoxia tolerant rodent nannospalax nehringi |
| topic | hypoxia no inos enos nnos mda nannospalax nehringi |
| url | https://vetdergikafkas.org/pdf.php?id=2457 |
| work_keys_str_mv | AT barısyildiz theeffectsofseverehypoxiaonnitricoxideparametersinhypoxiatolerantrodentnannospalaxnehringi AT nadidenabilkamiloglu theeffectsofseverehypoxiaonnitricoxideparametersinhypoxiatolerantrodentnannospalaxnehringi AT metinogun theeffectsofseverehypoxiaonnitricoxideparametersinhypoxiatolerantrodentnannospalaxnehringi AT cemozic theeffectsofseverehypoxiaonnitricoxideparametersinhypoxiatolerantrodentnannospalaxnehringi AT oguzmerhan theeffectsofseverehypoxiaonnitricoxideparametersinhypoxiatolerantrodentnannospalaxnehringi AT tarıkmecit theeffectsofseverehypoxiaonnitricoxideparametersinhypoxiatolerantrodentnannospalaxnehringi AT yukselcoskun theeffectsofseverehypoxiaonnitricoxideparametersinhypoxiatolerantrodentnannospalaxnehringi AT barısyildiz effectsofseverehypoxiaonnitricoxideparametersinhypoxiatolerantrodentnannospalaxnehringi AT nadidenabilkamiloglu effectsofseverehypoxiaonnitricoxideparametersinhypoxiatolerantrodentnannospalaxnehringi AT metinogun effectsofseverehypoxiaonnitricoxideparametersinhypoxiatolerantrodentnannospalaxnehringi AT cemozic effectsofseverehypoxiaonnitricoxideparametersinhypoxiatolerantrodentnannospalaxnehringi AT oguzmerhan effectsofseverehypoxiaonnitricoxideparametersinhypoxiatolerantrodentnannospalaxnehringi AT tarıkmecit effectsofseverehypoxiaonnitricoxideparametersinhypoxiatolerantrodentnannospalaxnehringi AT yukselcoskun effectsofseverehypoxiaonnitricoxideparametersinhypoxiatolerantrodentnannospalaxnehringi |