Application of a New Engineered Strain of <i>Yarrowia lipolytica</i> for Effective Production of Calcium Ketoglutarate Dietary Supplements
The present study aimed to develop a technology for the production of dietary supplements based on yeast biomass and α-ketoglutaric acid (KGA), produced by a new transformant of <i>Yarrowia lipolytica</i> with improved KGA biosynthesis ability, as well to verify the usefulness of the obt...
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| Format: | Article |
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MDPI AG
2021-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/14/7577 |
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| author | Ludwika Tomaszewska-Hetman Anita Rywińska Zbigniew Lazar Piotr Juszczyk Magdalena Rakicka-Pustułka Tomasz Janek Marta Kuźmińska-Bajor Waldemar Rymowicz |
| author_facet | Ludwika Tomaszewska-Hetman Anita Rywińska Zbigniew Lazar Piotr Juszczyk Magdalena Rakicka-Pustułka Tomasz Janek Marta Kuźmińska-Bajor Waldemar Rymowicz |
| author_sort | Ludwika Tomaszewska-Hetman |
| collection | DOAJ |
| description | The present study aimed to develop a technology for the production of dietary supplements based on yeast biomass and α-ketoglutaric acid (KGA), produced by a new transformant of <i>Yarrowia lipolytica</i> with improved KGA biosynthesis ability, as well to verify the usefulness of the obtained products for food and feed purposes. Transformants of <i>Y. lipolytica</i> were constructed to overexpress genes encoding glycerol kinase, methylcitrate synthase and mitochondrial organic acid transporter. The strains were compared in terms of growth ability in glycerol- and oil-based media as well as their suitability for KGA biosynthesis in mixed glycerol–oil medium. The impact of different C:N:P ratios on KGA production by selected strain was also evaluated. Application of the strain that overexpressed all three genes in the culture with a C:N:P ratio of 87:5:1 allowed us to obtain 53.1 g/L of KGA with productivity of 0.35 g/Lh and yield of 0.53 g/g. Finally, the possibility of obtaining three different products with desired nutritional and health-beneficial characteristics was demonstrated: (1) calcium α-ketoglutarate (CaKGA) with purity of 89.9% obtained by precipitation of KGA with CaCO<sub>3</sub>, (2) yeast biomass with very good nutritional properties, (3) fixed biomass-CaKGA preparation containing 87.2 μg/g of kynurenic acid, which increases the health-promoting value of the product. |
| first_indexed | 2024-03-10T09:37:08Z |
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| id | doaj.art-4266abc830fd44cab98e4c5d90a6f1ab |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T09:37:08Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4266abc830fd44cab98e4c5d90a6f1ab2023-11-22T04:00:46ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-012214757710.3390/ijms22147577Application of a New Engineered Strain of <i>Yarrowia lipolytica</i> for Effective Production of Calcium Ketoglutarate Dietary SupplementsLudwika Tomaszewska-Hetman0Anita Rywińska1Zbigniew Lazar2Piotr Juszczyk3Magdalena Rakicka-Pustułka4Tomasz Janek5Marta Kuźmińska-Bajor6Waldemar Rymowicz7Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Street 37, 51-630 Wrocław, PolandDepartment of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Street 37, 51-630 Wrocław, PolandDepartment of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Street 37, 51-630 Wrocław, PolandDepartment of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Street 37, 51-630 Wrocław, PolandDepartment of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Street 37, 51-630 Wrocław, PolandDepartment of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Street 37, 51-630 Wrocław, PolandDepartment of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Street 37, 51-630 Wrocław, PolandDepartment of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Street 37, 51-630 Wrocław, PolandThe present study aimed to develop a technology for the production of dietary supplements based on yeast biomass and α-ketoglutaric acid (KGA), produced by a new transformant of <i>Yarrowia lipolytica</i> with improved KGA biosynthesis ability, as well to verify the usefulness of the obtained products for food and feed purposes. Transformants of <i>Y. lipolytica</i> were constructed to overexpress genes encoding glycerol kinase, methylcitrate synthase and mitochondrial organic acid transporter. The strains were compared in terms of growth ability in glycerol- and oil-based media as well as their suitability for KGA biosynthesis in mixed glycerol–oil medium. The impact of different C:N:P ratios on KGA production by selected strain was also evaluated. Application of the strain that overexpressed all three genes in the culture with a C:N:P ratio of 87:5:1 allowed us to obtain 53.1 g/L of KGA with productivity of 0.35 g/Lh and yield of 0.53 g/g. Finally, the possibility of obtaining three different products with desired nutritional and health-beneficial characteristics was demonstrated: (1) calcium α-ketoglutarate (CaKGA) with purity of 89.9% obtained by precipitation of KGA with CaCO<sub>3</sub>, (2) yeast biomass with very good nutritional properties, (3) fixed biomass-CaKGA preparation containing 87.2 μg/g of kynurenic acid, which increases the health-promoting value of the product.https://www.mdpi.com/1422-0067/22/14/7577<i>Yarrowia lipolytica</i>α-ketoglutaric acid (KGA)calcium ketoglutaratedietary supplementmetabolic engineeringglycerol |
| spellingShingle | Ludwika Tomaszewska-Hetman Anita Rywińska Zbigniew Lazar Piotr Juszczyk Magdalena Rakicka-Pustułka Tomasz Janek Marta Kuźmińska-Bajor Waldemar Rymowicz Application of a New Engineered Strain of <i>Yarrowia lipolytica</i> for Effective Production of Calcium Ketoglutarate Dietary Supplements International Journal of Molecular Sciences <i>Yarrowia lipolytica</i> α-ketoglutaric acid (KGA) calcium ketoglutarate dietary supplement metabolic engineering glycerol |
| title | Application of a New Engineered Strain of <i>Yarrowia lipolytica</i> for Effective Production of Calcium Ketoglutarate Dietary Supplements |
| title_full | Application of a New Engineered Strain of <i>Yarrowia lipolytica</i> for Effective Production of Calcium Ketoglutarate Dietary Supplements |
| title_fullStr | Application of a New Engineered Strain of <i>Yarrowia lipolytica</i> for Effective Production of Calcium Ketoglutarate Dietary Supplements |
| title_full_unstemmed | Application of a New Engineered Strain of <i>Yarrowia lipolytica</i> for Effective Production of Calcium Ketoglutarate Dietary Supplements |
| title_short | Application of a New Engineered Strain of <i>Yarrowia lipolytica</i> for Effective Production of Calcium Ketoglutarate Dietary Supplements |
| title_sort | application of a new engineered strain of i yarrowia lipolytica i for effective production of calcium ketoglutarate dietary supplements |
| topic | <i>Yarrowia lipolytica</i> α-ketoglutaric acid (KGA) calcium ketoglutarate dietary supplement metabolic engineering glycerol |
| url | https://www.mdpi.com/1422-0067/22/14/7577 |
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