Gastric cancer cell supernatant causes apoptosis and fibrosis in the peritoneal tissues and results in an environment favorable to peritoneal metastases, <it>in vitro</it> and <it>in vivo</it>
<p>Abstract</p> <p>Background</p> <p>In this study, we examined effects of soluble factors released by gastric cancer cells on peritoneal mesothelial cells <it>in vitro</it> and <it>in vivo</it>.</p> <p>Methods</p> <p>HMrS...
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BMC
2012-04-01
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| Series: | BMC Gastroenterology |
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| Online Access: | http://www.biomedcentral.com/1471-230X/12/34 |
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| author | Na Di Lv Zhi-Dong Liu Fu-Nan Xu Yan Jiang Cheng-Gang Sun Zhe Miao Zhi-Feng Li Feng Xu Hui-Mian |
| author_facet | Na Di Lv Zhi-Dong Liu Fu-Nan Xu Yan Jiang Cheng-Gang Sun Zhe Miao Zhi-Feng Li Feng Xu Hui-Mian |
| author_sort | Na Di |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>In this study, we examined effects of soluble factors released by gastric cancer cells on peritoneal mesothelial cells <it>in vitro</it> and <it>in vivo</it>.</p> <p>Methods</p> <p>HMrSV5, a human peritoneal mesothelial cell line, was incubated with supernatants from gastric cancer cells. Morphological changes of HMrSV5 cells were observed. Apoptosis of HMrSV5 cells was observed under a transmission electron microscope and quantitatively determined by MTT assay and flow cytometry. Expressions of apoptosis-related proteins (caspase-3, caspase-8, Bax, bcl-2) were immunochemically evaluated.</p> <p>Results</p> <p>Conspicuous morphological changes indicating apoptosis were observed in HMrSV5 cells 24 h after treatment with the supernatants of gastric cancer cells. <it>In vivo</it>, peritoneal tissues treated with gastric cancer cell supernatant were substantially thickened and contained extensive fibrosis.</p> <p>Conclusions</p> <p>These findings demonstrate that supernatants of gastric cancer cells can induce apoptosis and fibrosis in HMrSV5 human peritoneal mesothelial cells through supernatants in the early peritoneal metastasis, in a time-dependent manner, and indicate that soluble factors in the peritoneal cavity affect the morphology and function of mesothelial cells so that the resulting environment can become favorable to peritoneal metastases.</p> |
| first_indexed | 2024-12-12T21:22:49Z |
| format | Article |
| id | doaj.art-426abafd353d447b9e1a8f31212e57d6 |
| institution | Directory Open Access Journal |
| issn | 1471-230X |
| language | English |
| last_indexed | 2024-12-12T21:22:49Z |
| publishDate | 2012-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj.art-426abafd353d447b9e1a8f31212e57d62022-12-22T00:11:31ZengBMCBMC Gastroenterology1471-230X2012-04-011213410.1186/1471-230X-12-34Gastric cancer cell supernatant causes apoptosis and fibrosis in the peritoneal tissues and results in an environment favorable to peritoneal metastases, <it>in vitro</it> and <it>in vivo</it>Na DiLv Zhi-DongLiu Fu-NanXu YanJiang Cheng-GangSun ZheMiao Zhi-FengLi FengXu Hui-Mian<p>Abstract</p> <p>Background</p> <p>In this study, we examined effects of soluble factors released by gastric cancer cells on peritoneal mesothelial cells <it>in vitro</it> and <it>in vivo</it>.</p> <p>Methods</p> <p>HMrSV5, a human peritoneal mesothelial cell line, was incubated with supernatants from gastric cancer cells. Morphological changes of HMrSV5 cells were observed. Apoptosis of HMrSV5 cells was observed under a transmission electron microscope and quantitatively determined by MTT assay and flow cytometry. Expressions of apoptosis-related proteins (caspase-3, caspase-8, Bax, bcl-2) were immunochemically evaluated.</p> <p>Results</p> <p>Conspicuous morphological changes indicating apoptosis were observed in HMrSV5 cells 24 h after treatment with the supernatants of gastric cancer cells. <it>In vivo</it>, peritoneal tissues treated with gastric cancer cell supernatant were substantially thickened and contained extensive fibrosis.</p> <p>Conclusions</p> <p>These findings demonstrate that supernatants of gastric cancer cells can induce apoptosis and fibrosis in HMrSV5 human peritoneal mesothelial cells through supernatants in the early peritoneal metastasis, in a time-dependent manner, and indicate that soluble factors in the peritoneal cavity affect the morphology and function of mesothelial cells so that the resulting environment can become favorable to peritoneal metastases.</p>http://www.biomedcentral.com/1471-230X/12/34Peritoneal carcinomatosisStomach neoplasmsMesothelial cellApoptosisFibrosis |
| spellingShingle | Na Di Lv Zhi-Dong Liu Fu-Nan Xu Yan Jiang Cheng-Gang Sun Zhe Miao Zhi-Feng Li Feng Xu Hui-Mian Gastric cancer cell supernatant causes apoptosis and fibrosis in the peritoneal tissues and results in an environment favorable to peritoneal metastases, <it>in vitro</it> and <it>in vivo</it> BMC Gastroenterology Peritoneal carcinomatosis Stomach neoplasms Mesothelial cell Apoptosis Fibrosis |
| title | Gastric cancer cell supernatant causes apoptosis and fibrosis in the peritoneal tissues and results in an environment favorable to peritoneal metastases, <it>in vitro</it> and <it>in vivo</it> |
| title_full | Gastric cancer cell supernatant causes apoptosis and fibrosis in the peritoneal tissues and results in an environment favorable to peritoneal metastases, <it>in vitro</it> and <it>in vivo</it> |
| title_fullStr | Gastric cancer cell supernatant causes apoptosis and fibrosis in the peritoneal tissues and results in an environment favorable to peritoneal metastases, <it>in vitro</it> and <it>in vivo</it> |
| title_full_unstemmed | Gastric cancer cell supernatant causes apoptosis and fibrosis in the peritoneal tissues and results in an environment favorable to peritoneal metastases, <it>in vitro</it> and <it>in vivo</it> |
| title_short | Gastric cancer cell supernatant causes apoptosis and fibrosis in the peritoneal tissues and results in an environment favorable to peritoneal metastases, <it>in vitro</it> and <it>in vivo</it> |
| title_sort | gastric cancer cell supernatant causes apoptosis and fibrosis in the peritoneal tissues and results in an environment favorable to peritoneal metastases it in vitro it and it in vivo it |
| topic | Peritoneal carcinomatosis Stomach neoplasms Mesothelial cell Apoptosis Fibrosis |
| url | http://www.biomedcentral.com/1471-230X/12/34 |
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