miR-29b inhibits TGF-β1-induced cell proliferation in articular chondrocytes
Transforming growth factor β1 (TGF-β1) is a known regulator of chondrocyte proliferation and promotes cartilage repair in osteoarthritis (OA). microRNA-29b-3p (miR-29b-3p) is downregulated by TGF-β1 and overexpressed in OA cartilage. However, the ability of miR-29b-3p to mediate the chondrocyte pro-...
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| Format: | Article |
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Elsevier
2022-03-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580822000176 |
| _version_ | 1818683213399195648 |
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| author | Masahiro Horita Shun-Neng Hsu Anna Raper Colin Farquharson Louise A. Stephen |
| author_facet | Masahiro Horita Shun-Neng Hsu Anna Raper Colin Farquharson Louise A. Stephen |
| author_sort | Masahiro Horita |
| collection | DOAJ |
| description | Transforming growth factor β1 (TGF-β1) is a known regulator of chondrocyte proliferation and promotes cartilage repair in osteoarthritis (OA). microRNA-29b-3p (miR-29b-3p) is downregulated by TGF-β1 and overexpressed in OA cartilage. However, the ability of miR-29b-3p to mediate the chondrocyte pro-proliferative effects of TGF-β1 is not yet understood. This current study aimed to investigate the effect of miR-29b-3p on TGF-β1-induced cell proliferation in murine articular chondrocytes. The stimulation of chondrocytes by TGF-β1 for 24 h resulted in the downregulation of miR-29b-3p expression. The ratio of G0/G1 phase cells decreased in response to TGF-β1 whereas the ratio of S phase cells was increased. Consistent with this observation, miR-29b-3p overexpression inhibited TGF-β1’s ability to promote the ratio of S phase cells and downregulate the ratio of G0/G1 phase cells. These findings suggest that the downregulation of miR-29b-3p is a likely requirement for TGF-β1-mediated proliferation of murine articular chondrocytes. Furthermore, implying that miR-29b-3p expression may be involved in reduced chondrocyte proliferation in OA. |
| first_indexed | 2024-12-17T10:31:10Z |
| format | Article |
| id | doaj.art-426ee393ce964d6c95b4d91947d1382a |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-17T10:31:10Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-426ee393ce964d6c95b4d91947d1382a2022-12-21T21:52:31ZengElsevierBiochemistry and Biophysics Reports2405-58082022-03-0129101216miR-29b inhibits TGF-β1-induced cell proliferation in articular chondrocytesMasahiro Horita0Shun-Neng Hsu1Anna Raper2Colin Farquharson3Louise A. Stephen4The Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, Scotland, UK; Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan; Corresponding author. The Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, Scotland, UK.The Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, Scotland, UKThe Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, Scotland, UKThe Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, Scotland, UKThe Roslin Institute and Royal (Dick) School of Veterinary Studies, The University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, Scotland, UKTransforming growth factor β1 (TGF-β1) is a known regulator of chondrocyte proliferation and promotes cartilage repair in osteoarthritis (OA). microRNA-29b-3p (miR-29b-3p) is downregulated by TGF-β1 and overexpressed in OA cartilage. However, the ability of miR-29b-3p to mediate the chondrocyte pro-proliferative effects of TGF-β1 is not yet understood. This current study aimed to investigate the effect of miR-29b-3p on TGF-β1-induced cell proliferation in murine articular chondrocytes. The stimulation of chondrocytes by TGF-β1 for 24 h resulted in the downregulation of miR-29b-3p expression. The ratio of G0/G1 phase cells decreased in response to TGF-β1 whereas the ratio of S phase cells was increased. Consistent with this observation, miR-29b-3p overexpression inhibited TGF-β1’s ability to promote the ratio of S phase cells and downregulate the ratio of G0/G1 phase cells. These findings suggest that the downregulation of miR-29b-3p is a likely requirement for TGF-β1-mediated proliferation of murine articular chondrocytes. Furthermore, implying that miR-29b-3p expression may be involved in reduced chondrocyte proliferation in OA.http://www.sciencedirect.com/science/article/pii/S2405580822000176ChondrocyteTGF-β1microRNA-29b-3pOsteoarthritisCell proliferation |
| spellingShingle | Masahiro Horita Shun-Neng Hsu Anna Raper Colin Farquharson Louise A. Stephen miR-29b inhibits TGF-β1-induced cell proliferation in articular chondrocytes Biochemistry and Biophysics Reports Chondrocyte TGF-β1 microRNA-29b-3p Osteoarthritis Cell proliferation |
| title | miR-29b inhibits TGF-β1-induced cell proliferation in articular chondrocytes |
| title_full | miR-29b inhibits TGF-β1-induced cell proliferation in articular chondrocytes |
| title_fullStr | miR-29b inhibits TGF-β1-induced cell proliferation in articular chondrocytes |
| title_full_unstemmed | miR-29b inhibits TGF-β1-induced cell proliferation in articular chondrocytes |
| title_short | miR-29b inhibits TGF-β1-induced cell proliferation in articular chondrocytes |
| title_sort | mir 29b inhibits tgf β1 induced cell proliferation in articular chondrocytes |
| topic | Chondrocyte TGF-β1 microRNA-29b-3p Osteoarthritis Cell proliferation |
| url | http://www.sciencedirect.com/science/article/pii/S2405580822000176 |
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