Transfer RNAs Mediate the Rapid Adaptation of Escherichia coli to Oxidative Stress.
Translational systems can respond promptly to sudden environmental changes to provide rapid adaptations to environmental stress. Unlike the well-studied translational responses to oxidative stress in eukaryotic systems, little is known regarding how prokaryotes respond rapidly to oxidative stress in...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-06-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC4474833?pdf=render |
| _version_ | 1828380087026712576 |
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| author | Jiayong Zhong Chuanle Xiao Wei Gu Gaofei Du Xuesong Sun Qing-Yu He Gong Zhang |
| author_facet | Jiayong Zhong Chuanle Xiao Wei Gu Gaofei Du Xuesong Sun Qing-Yu He Gong Zhang |
| author_sort | Jiayong Zhong |
| collection | DOAJ |
| description | Translational systems can respond promptly to sudden environmental changes to provide rapid adaptations to environmental stress. Unlike the well-studied translational responses to oxidative stress in eukaryotic systems, little is known regarding how prokaryotes respond rapidly to oxidative stress in terms of translation. In this study, we measured protein synthesis from the entire Escherichia coli proteome and found that protein synthesis was severely slowed down under oxidative stress. With unchanged translation initiation, this slowdown was caused by decreased translation elongation speed. We further confirmed by tRNA sequencing and qRT-PCR that this deceleration was caused by a global, enzymatic downregulation of almost all tRNA species shortly after exposure to oxidative agents. Elevation in tRNA levels accelerated translation and protected E. coli against oxidative stress caused by hydrogen peroxide and the antibiotic ciprofloxacin. Our results showed that the global regulation of tRNAs mediates the rapid adjustment of the E. coli translation system for prompt adaptation to oxidative stress. |
| first_indexed | 2024-12-10T03:51:13Z |
| format | Article |
| id | doaj.art-426ffd3281394653b600b5d1d48a9103 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-10T03:51:13Z |
| publishDate | 2015-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-426ffd3281394653b600b5d1d48a91032022-12-22T02:03:14ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042015-06-01116e100530210.1371/journal.pgen.1005302Transfer RNAs Mediate the Rapid Adaptation of Escherichia coli to Oxidative Stress.Jiayong ZhongChuanle XiaoWei GuGaofei DuXuesong SunQing-Yu HeGong ZhangTranslational systems can respond promptly to sudden environmental changes to provide rapid adaptations to environmental stress. Unlike the well-studied translational responses to oxidative stress in eukaryotic systems, little is known regarding how prokaryotes respond rapidly to oxidative stress in terms of translation. In this study, we measured protein synthesis from the entire Escherichia coli proteome and found that protein synthesis was severely slowed down under oxidative stress. With unchanged translation initiation, this slowdown was caused by decreased translation elongation speed. We further confirmed by tRNA sequencing and qRT-PCR that this deceleration was caused by a global, enzymatic downregulation of almost all tRNA species shortly after exposure to oxidative agents. Elevation in tRNA levels accelerated translation and protected E. coli against oxidative stress caused by hydrogen peroxide and the antibiotic ciprofloxacin. Our results showed that the global regulation of tRNAs mediates the rapid adjustment of the E. coli translation system for prompt adaptation to oxidative stress.http://europepmc.org/articles/PMC4474833?pdf=render |
| spellingShingle | Jiayong Zhong Chuanle Xiao Wei Gu Gaofei Du Xuesong Sun Qing-Yu He Gong Zhang Transfer RNAs Mediate the Rapid Adaptation of Escherichia coli to Oxidative Stress. PLoS Genetics |
| title | Transfer RNAs Mediate the Rapid Adaptation of Escherichia coli to Oxidative Stress. |
| title_full | Transfer RNAs Mediate the Rapid Adaptation of Escherichia coli to Oxidative Stress. |
| title_fullStr | Transfer RNAs Mediate the Rapid Adaptation of Escherichia coli to Oxidative Stress. |
| title_full_unstemmed | Transfer RNAs Mediate the Rapid Adaptation of Escherichia coli to Oxidative Stress. |
| title_short | Transfer RNAs Mediate the Rapid Adaptation of Escherichia coli to Oxidative Stress. |
| title_sort | transfer rnas mediate the rapid adaptation of escherichia coli to oxidative stress |
| url | http://europepmc.org/articles/PMC4474833?pdf=render |
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