Silencing Folylpolyglutamate Synthetase1 (FPGS1) in Switchgrass (Panicum virgatum L.) Improves Lignocellulosic Biofuel Production
Switchgrass (Panicum virgatum L.) is a lignocellulosic perennial grass with great potential in bioenergy field. Lignocellulosic bioenergy crops are mostly resistant to cell wall deconstruction, and therefore yield suboptimal levels of biofuel. The one-carbon pathway (also known as C1 metabolism) is...
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.00843/full |
| _version_ | 1819153604261445632 |
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| author | Mitra Mazarei Mitra Mazarei Mitra Mazarei Holly L. Baxter Holly L. Baxter Avinash Srivastava Avinash Srivastava Guifen Li Guifen Li Hongli Xie Hongli Xie Alexandru Dumitrache Alexandru Dumitrache Miguel Rodriguez Miguel Rodriguez Miguel Rodriguez Jace M. Natzke Jace M. Natzke Ji-Yi Zhang Ji-Yi Zhang Geoffrey B. Turner Geoffrey B. Turner Robert W. Sykes Robert W. Sykes Mark F. Davis Mark F. Davis Mark F. Davis Michael K. Udvardi Michael K. Udvardi Michael K. Udvardi Zeng-Yu Wang Zeng-Yu Wang Brian H. Davison Brian H. Davison Brian H. Davison Elison B. Blancaflor Elison B. Blancaflor Yuhong Tang Yuhong Tang Charles Neal Stewart Charles Neal Stewart Charles Neal Stewart |
| author_facet | Mitra Mazarei Mitra Mazarei Mitra Mazarei Holly L. Baxter Holly L. Baxter Avinash Srivastava Avinash Srivastava Guifen Li Guifen Li Hongli Xie Hongli Xie Alexandru Dumitrache Alexandru Dumitrache Miguel Rodriguez Miguel Rodriguez Miguel Rodriguez Jace M. Natzke Jace M. Natzke Ji-Yi Zhang Ji-Yi Zhang Geoffrey B. Turner Geoffrey B. Turner Robert W. Sykes Robert W. Sykes Mark F. Davis Mark F. Davis Mark F. Davis Michael K. Udvardi Michael K. Udvardi Michael K. Udvardi Zeng-Yu Wang Zeng-Yu Wang Brian H. Davison Brian H. Davison Brian H. Davison Elison B. Blancaflor Elison B. Blancaflor Yuhong Tang Yuhong Tang Charles Neal Stewart Charles Neal Stewart Charles Neal Stewart |
| author_sort | Mitra Mazarei |
| collection | DOAJ |
| description | Switchgrass (Panicum virgatum L.) is a lignocellulosic perennial grass with great potential in bioenergy field. Lignocellulosic bioenergy crops are mostly resistant to cell wall deconstruction, and therefore yield suboptimal levels of biofuel. The one-carbon pathway (also known as C1 metabolism) is critical for polymer methylation, including that of lignin and hemicelluloses in cell walls. Folylpolyglutamate synthetase (FPGS) catalyzes a biochemical reaction that leads to the formation of folylpolyglutamate, an important cofactor for many enzymes in the C1 pathway. In this study, the putatively novel switchgrass PvFPGS1 gene was identified and its functional role in cell wall composition and biofuel production was examined by RNAi knockdown analysis. The PvFPGS1-downregulated plants were analyzed in the field over three growing seasons. Transgenic plants with the highest reduction in PvFPGS1 expression grew slower and produced lower end-of-season biomass. Transgenic plants with low-to-moderate reduction in PvFPGS1 transcript levels produced equivalent biomass as controls. There were no significant differences observed for lignin content and syringyl/guaiacyl lignin monomer ratio in the low-to-moderately reduced PvFPGS1 transgenic lines compared with the controls. Similarly, sugar release efficiency was also not significantly different in these transgenic lines compared with the control lines. However, transgenic plants produced up to 18% more ethanol while maintaining congruent growth and biomass as non-transgenic controls. Severity of rust disease among transgenic and control lines were not different during the time course of the field experiments. Altogether, the unchanged lignin content and composition in the low-to-moderate PvFPGS1-downregulated lines may suggest that partial downregulation of PvFPGS1 expression did not impact lignin biosynthesis in switchgrass. In conclusion, the manipulation of PvFPGS1 expression in bioenergy crops may be useful to increase biofuel potential with no growth penalty or increased susceptibility to rust in feedstock. |
| first_indexed | 2024-12-22T15:07:49Z |
| format | Article |
| id | doaj.art-427088f1a4834c50a7346fd1fb79b919 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-22T15:07:49Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-427088f1a4834c50a7346fd1fb79b9192022-12-21T18:21:56ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-06-011110.3389/fpls.2020.00843545931Silencing Folylpolyglutamate Synthetase1 (FPGS1) in Switchgrass (Panicum virgatum L.) Improves Lignocellulosic Biofuel ProductionMitra Mazarei0Mitra Mazarei1Mitra Mazarei2Holly L. Baxter3Holly L. Baxter4Avinash Srivastava5Avinash Srivastava6Guifen Li7Guifen Li8Hongli Xie9Hongli Xie10Alexandru Dumitrache11Alexandru Dumitrache12Miguel Rodriguez13Miguel Rodriguez14Miguel Rodriguez15Jace M. Natzke16Jace M. Natzke17Ji-Yi Zhang18Ji-Yi Zhang19Geoffrey B. Turner20Geoffrey B. Turner21Robert W. Sykes22Robert W. Sykes23Mark F. Davis24Mark F. Davis25Mark F. Davis26Michael K. Udvardi27Michael K. Udvardi28Michael K. Udvardi29Zeng-Yu Wang30Zeng-Yu Wang31Brian H. Davison32Brian H. Davison33Brian H. Davison34Elison B. Blancaflor35Elison B. Blancaflor36Yuhong Tang37Yuhong Tang38Charles Neal Stewart39Charles Neal Stewart40Charles Neal Stewart41Department of Plant Sciences, The University of Tennessee, Knoxville, TN, United StatesBioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesThe Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesDepartment of Plant Sciences, The University of Tennessee, Knoxville, TN, United StatesBioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesBioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesNoble Research Institute, Ardmore, OK, United StatesBioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesNoble Research Institute, Ardmore, OK, United StatesBioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesNoble Research Institute, Ardmore, OK, United StatesBioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesBiosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesBioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesThe Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesBiosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesBioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesBiosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesBioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesNoble Research Institute, Ardmore, OK, United StatesBioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesNational Renewable Energy Laboratory, Golden, CO, United StatesBioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesNational Renewable Energy Laboratory, Golden, CO, United StatesBioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesThe Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesNational Renewable Energy Laboratory, Golden, CO, United StatesBioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesThe Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesNoble Research Institute, Ardmore, OK, United StatesBioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesNoble Research Institute, Ardmore, OK, United StatesBioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesThe Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesBiosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesBioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesNoble Research Institute, Ardmore, OK, United StatesBioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesNoble Research Institute, Ardmore, OK, United StatesDepartment of Plant Sciences, The University of Tennessee, Knoxville, TN, United StatesBioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesThe Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN, United StatesSwitchgrass (Panicum virgatum L.) is a lignocellulosic perennial grass with great potential in bioenergy field. Lignocellulosic bioenergy crops are mostly resistant to cell wall deconstruction, and therefore yield suboptimal levels of biofuel. The one-carbon pathway (also known as C1 metabolism) is critical for polymer methylation, including that of lignin and hemicelluloses in cell walls. Folylpolyglutamate synthetase (FPGS) catalyzes a biochemical reaction that leads to the formation of folylpolyglutamate, an important cofactor for many enzymes in the C1 pathway. In this study, the putatively novel switchgrass PvFPGS1 gene was identified and its functional role in cell wall composition and biofuel production was examined by RNAi knockdown analysis. The PvFPGS1-downregulated plants were analyzed in the field over three growing seasons. Transgenic plants with the highest reduction in PvFPGS1 expression grew slower and produced lower end-of-season biomass. Transgenic plants with low-to-moderate reduction in PvFPGS1 transcript levels produced equivalent biomass as controls. There were no significant differences observed for lignin content and syringyl/guaiacyl lignin monomer ratio in the low-to-moderately reduced PvFPGS1 transgenic lines compared with the controls. Similarly, sugar release efficiency was also not significantly different in these transgenic lines compared with the control lines. However, transgenic plants produced up to 18% more ethanol while maintaining congruent growth and biomass as non-transgenic controls. Severity of rust disease among transgenic and control lines were not different during the time course of the field experiments. Altogether, the unchanged lignin content and composition in the low-to-moderate PvFPGS1-downregulated lines may suggest that partial downregulation of PvFPGS1 expression did not impact lignin biosynthesis in switchgrass. In conclusion, the manipulation of PvFPGS1 expression in bioenergy crops may be useful to increase biofuel potential with no growth penalty or increased susceptibility to rust in feedstock.https://www.frontiersin.org/article/10.3389/fpls.2020.00843/fullfolylpolyglutamate synthetaseswitchgrassRNAi-gene silencingPvFPGS1lignocellulosicbiofuel |
| spellingShingle | Mitra Mazarei Mitra Mazarei Mitra Mazarei Holly L. Baxter Holly L. Baxter Avinash Srivastava Avinash Srivastava Guifen Li Guifen Li Hongli Xie Hongli Xie Alexandru Dumitrache Alexandru Dumitrache Miguel Rodriguez Miguel Rodriguez Miguel Rodriguez Jace M. Natzke Jace M. Natzke Ji-Yi Zhang Ji-Yi Zhang Geoffrey B. Turner Geoffrey B. Turner Robert W. Sykes Robert W. Sykes Mark F. Davis Mark F. Davis Mark F. Davis Michael K. Udvardi Michael K. Udvardi Michael K. Udvardi Zeng-Yu Wang Zeng-Yu Wang Brian H. Davison Brian H. Davison Brian H. Davison Elison B. Blancaflor Elison B. Blancaflor Yuhong Tang Yuhong Tang Charles Neal Stewart Charles Neal Stewart Charles Neal Stewart Silencing Folylpolyglutamate Synthetase1 (FPGS1) in Switchgrass (Panicum virgatum L.) Improves Lignocellulosic Biofuel Production Frontiers in Plant Science folylpolyglutamate synthetase switchgrass RNAi-gene silencing PvFPGS1 lignocellulosic biofuel |
| title | Silencing Folylpolyglutamate Synthetase1 (FPGS1) in Switchgrass (Panicum virgatum L.) Improves Lignocellulosic Biofuel Production |
| title_full | Silencing Folylpolyglutamate Synthetase1 (FPGS1) in Switchgrass (Panicum virgatum L.) Improves Lignocellulosic Biofuel Production |
| title_fullStr | Silencing Folylpolyglutamate Synthetase1 (FPGS1) in Switchgrass (Panicum virgatum L.) Improves Lignocellulosic Biofuel Production |
| title_full_unstemmed | Silencing Folylpolyglutamate Synthetase1 (FPGS1) in Switchgrass (Panicum virgatum L.) Improves Lignocellulosic Biofuel Production |
| title_short | Silencing Folylpolyglutamate Synthetase1 (FPGS1) in Switchgrass (Panicum virgatum L.) Improves Lignocellulosic Biofuel Production |
| title_sort | silencing folylpolyglutamate synthetase1 fpgs1 in switchgrass panicum virgatum l improves lignocellulosic biofuel production |
| topic | folylpolyglutamate synthetase switchgrass RNAi-gene silencing PvFPGS1 lignocellulosic biofuel |
| url | https://www.frontiersin.org/article/10.3389/fpls.2020.00843/full |
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