Toxicities of synthesized green iron oxide nanoparticles on Trialeurodes vaporariorum and their effects on antioxidant enzymes and lipid peroxidation
In this study, lethal concentrations of iron-oxide nanoparticles (FeONPs) synthesized from plants (alfalfa, basil, eucalyptus, cinnamon) were investigated for their effects on mortality, antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and gluta...
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| Format: | Article |
| Language: | English |
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Entomological Society of Iran
2023-06-01
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| Series: | نامه انجمن حشرهشناسی ایران |
| Subjects: | |
| Online Access: | https://jesi.areeo.ac.ir/article_129265_df9217899e282e5857452f188f282c8f.pdf |
| _version_ | 1797769965402587136 |
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| author | Najmeh Sahebzadeh Tayebeh Haddadi Azizollah Mokhtari |
| author_facet | Najmeh Sahebzadeh Tayebeh Haddadi Azizollah Mokhtari |
| author_sort | Najmeh Sahebzadeh |
| collection | DOAJ |
| description | In this study, lethal concentrations of iron-oxide nanoparticles (FeONPs) synthesized from plants (alfalfa, basil, eucalyptus, cinnamon) were investigated for their effects on mortality, antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferases (GST)) and lipid peroxidation (LPO) to develop an effective and inexpensive method for controlling the economic losses caused by Trialeurodes vaporariorum. The characterization of prepared FeONPs with plant extracts was performed using an ultraviolet-visible spectrophotometer (UV-Vis), Fourier transforms infrared spectroscopy (FTIR), and field emission scanning electron microscopy (FESEM) to examine the size, elemental composition, and morphology. The UV-Vis results of the green synthesized FeONP showed a peak at 256 nm, confirming the synthesis of FeONPs. An FTIR detected strong absorption peaks at 3420, 3446, 3421, and 3446 cm−1 (hydroxyl group); 2925 and 2926 cm−1 (C–H group); 1618, 1636, 1647, and 1716 cm−1 (C=O group), at approximately 1030, 1034, 1044, and 1048 cm−1 (C–O group). Bioassay tests have been conducted with adults of T. vaporariorum in plastic leaf cages treated with different concentrations of FeONPs. SPSS 21 software (IBM, New York, US) with a confidence interval (CI) of 95% and Probit analysis was employed to determine lethal concentrations (LC10, LC25, LC30, and LC50) of synthesized FeONPs. The results demonstrated that basil, alfalfa, eucalyptus and cinnamon FeONPs effectively killed 50% of T.vaporariorum adults, at concentrations of 4.876, 16.935, 10.584 and 11.948 mg L-1, respectively. Significant increases in LPO, GST, GPX, CAT, and SOD activities were observed in T.vaporariorum adults exposed to the lethal concentrations of different FeONPs. Moreover, the findings suggested that exposure to FeONPs induced oxidative stress in T. vaporariorum adults and may decline their longevity in greenhouses. |
| first_indexed | 2024-03-12T21:15:44Z |
| format | Article |
| id | doaj.art-4271e26bb3dd46fa9aaf183c7fc1cac0 |
| institution | Directory Open Access Journal |
| issn | 0259-9996 2783-3968 |
| language | English |
| last_indexed | 2024-03-12T21:15:44Z |
| publishDate | 2023-06-01 |
| publisher | Entomological Society of Iran |
| record_format | Article |
| series | نامه انجمن حشرهشناسی ایران |
| spelling | doaj.art-4271e26bb3dd46fa9aaf183c7fc1cac02023-07-29T08:07:45ZengEntomological Society of Iranنامه انجمن حشرهشناسی ایران0259-99962783-39682023-06-0143214916510.52547/jesi.43.2.6129265Toxicities of synthesized green iron oxide nanoparticles on Trialeurodes vaporariorum and their effects on antioxidant enzymes and lipid peroxidationNajmeh Sahebzadeh0Tayebeh Haddadi1Azizollah Mokhtari2Department of Plant Protection, Faculty of Agriculture, University of Zabol, Zabol, Sisan and Baluchestan, IranDepartment of Food Science, Faculty of Agriculture, University of Zabol, Zabol, Sisan and Baluchestan, IranDepartment of Plant Protection, Faculty of Agriculture, University of Zabol, Zabol, Sisan and Baluchestan, IranIn this study, lethal concentrations of iron-oxide nanoparticles (FeONPs) synthesized from plants (alfalfa, basil, eucalyptus, cinnamon) were investigated for their effects on mortality, antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferases (GST)) and lipid peroxidation (LPO) to develop an effective and inexpensive method for controlling the economic losses caused by Trialeurodes vaporariorum. The characterization of prepared FeONPs with plant extracts was performed using an ultraviolet-visible spectrophotometer (UV-Vis), Fourier transforms infrared spectroscopy (FTIR), and field emission scanning electron microscopy (FESEM) to examine the size, elemental composition, and morphology. The UV-Vis results of the green synthesized FeONP showed a peak at 256 nm, confirming the synthesis of FeONPs. An FTIR detected strong absorption peaks at 3420, 3446, 3421, and 3446 cm−1 (hydroxyl group); 2925 and 2926 cm−1 (C–H group); 1618, 1636, 1647, and 1716 cm−1 (C=O group), at approximately 1030, 1034, 1044, and 1048 cm−1 (C–O group). Bioassay tests have been conducted with adults of T. vaporariorum in plastic leaf cages treated with different concentrations of FeONPs. SPSS 21 software (IBM, New York, US) with a confidence interval (CI) of 95% and Probit analysis was employed to determine lethal concentrations (LC10, LC25, LC30, and LC50) of synthesized FeONPs. The results demonstrated that basil, alfalfa, eucalyptus and cinnamon FeONPs effectively killed 50% of T.vaporariorum adults, at concentrations of 4.876, 16.935, 10.584 and 11.948 mg L-1, respectively. Significant increases in LPO, GST, GPX, CAT, and SOD activities were observed in T.vaporariorum adults exposed to the lethal concentrations of different FeONPs. Moreover, the findings suggested that exposure to FeONPs induced oxidative stress in T. vaporariorum adults and may decline their longevity in greenhouses.https://jesi.areeo.ac.ir/article_129265_df9217899e282e5857452f188f282c8f.pdfbiomarkerenzymatic antioxidantsnanotoxicologyoxidative stressreactive oxygen species |
| spellingShingle | Najmeh Sahebzadeh Tayebeh Haddadi Azizollah Mokhtari Toxicities of synthesized green iron oxide nanoparticles on Trialeurodes vaporariorum and their effects on antioxidant enzymes and lipid peroxidation نامه انجمن حشرهشناسی ایران biomarker enzymatic antioxidants nanotoxicology oxidative stress reactive oxygen species |
| title | Toxicities of synthesized green iron oxide nanoparticles on Trialeurodes vaporariorum and their effects on antioxidant enzymes and lipid peroxidation |
| title_full | Toxicities of synthesized green iron oxide nanoparticles on Trialeurodes vaporariorum and their effects on antioxidant enzymes and lipid peroxidation |
| title_fullStr | Toxicities of synthesized green iron oxide nanoparticles on Trialeurodes vaporariorum and their effects on antioxidant enzymes and lipid peroxidation |
| title_full_unstemmed | Toxicities of synthesized green iron oxide nanoparticles on Trialeurodes vaporariorum and their effects on antioxidant enzymes and lipid peroxidation |
| title_short | Toxicities of synthesized green iron oxide nanoparticles on Trialeurodes vaporariorum and their effects on antioxidant enzymes and lipid peroxidation |
| title_sort | toxicities of synthesized green iron oxide nanoparticles on trialeurodes vaporariorum and their effects on antioxidant enzymes and lipid peroxidation |
| topic | biomarker enzymatic antioxidants nanotoxicology oxidative stress reactive oxygen species |
| url | https://jesi.areeo.ac.ir/article_129265_df9217899e282e5857452f188f282c8f.pdf |
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