Protective Effects of Polyphenol of Lotus Seed Epicarp on Oxidative Stress Damage Induced by T-BHP
Objective: The protective effect of polyphenol of lotus seed epicarp on HepG2 oxidative stress damage induced by tert-butylhydro-peroxide (T-BHP) was investigated. Method: The concentration of T-BHP with a survival rate close to 50% was selected as the concentration for establishing the oxidative da...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2023-06-01
|
| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022070173 |
| _version_ | 1797737835952865280 |
|---|---|
| author | Yajun LU Ying LIU Yi WANG Wen HUANG |
| author_facet | Yajun LU Ying LIU Yi WANG Wen HUANG |
| author_sort | Yajun LU |
| collection | DOAJ |
| description | Objective: The protective effect of polyphenol of lotus seed epicarp on HepG2 oxidative stress damage induced by tert-butylhydro-peroxide (T-BHP) was investigated. Method: The concentration of T-BHP with a survival rate close to 50% was selected as the concentration for establishing the oxidative damage model. To evaluate the anti-oxidative stress activity levels of polyphenol of lotus seed epicarp at different concentrations (2.5, 5, and 7.5 μg/mL), cell viability, reactive oxygen species (ROS), malondialdehyde (MDA), lactic dehydrogenase (LDH), glutathione (GSH) and antioxidant enzyme-related gene expression were used as evaluation indicators, and tea polyphenol was used as the positive control. Result: When 120 μmol/L concentration of T-BHP was established for 4 h, the cell survival rate reached 49.18%±7.55%, which met the requirements of model construction. Therefore, 120 μmol/L was selected as the modeling concentration of the oxidative damage model for subsequent experiments. The polyphenol from lotus seed epicarp significantly inhibited the decrease of cell survival rate induced by T-BHP (P<0.01). At 7.5 μg/mL, the ROS level decreased by 45.99%, the MDA decreased by 58.77%, the LDH decreased by 71.61%, and the GSH increased by 206.60% (P<0.05). The expression of antioxidant enzyme-related genes including CuZnSOD, GCLC, GPx, and GSH also gradually returned to the normal level, all in a dose-dependent manner. Conclusion: The polyphenol of lotus seed epicarp protected against the oxidative damage induced by T-BHP in HepG2 cells. The mechanism might be related to the increase of intracellular antioxidant enzyme activity and the clearance of excessive ROS. |
| first_indexed | 2024-03-12T13:34:13Z |
| format | Article |
| id | doaj.art-42720cbf95cc46d4b4a320e5a8f89518 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-12T13:34:13Z |
| publishDate | 2023-06-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-42720cbf95cc46d4b4a320e5a8f895182023-08-24T06:00:59ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-06-01441239740410.13386/j.issn1002-0306.20220701732022070173-12Protective Effects of Polyphenol of Lotus Seed Epicarp on Oxidative Stress Damage Induced by T-BHPYajun LU0Ying LIU1Yi WANG2Wen HUANG3College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaObjective: The protective effect of polyphenol of lotus seed epicarp on HepG2 oxidative stress damage induced by tert-butylhydro-peroxide (T-BHP) was investigated. Method: The concentration of T-BHP with a survival rate close to 50% was selected as the concentration for establishing the oxidative damage model. To evaluate the anti-oxidative stress activity levels of polyphenol of lotus seed epicarp at different concentrations (2.5, 5, and 7.5 μg/mL), cell viability, reactive oxygen species (ROS), malondialdehyde (MDA), lactic dehydrogenase (LDH), glutathione (GSH) and antioxidant enzyme-related gene expression were used as evaluation indicators, and tea polyphenol was used as the positive control. Result: When 120 μmol/L concentration of T-BHP was established for 4 h, the cell survival rate reached 49.18%±7.55%, which met the requirements of model construction. Therefore, 120 μmol/L was selected as the modeling concentration of the oxidative damage model for subsequent experiments. The polyphenol from lotus seed epicarp significantly inhibited the decrease of cell survival rate induced by T-BHP (P<0.01). At 7.5 μg/mL, the ROS level decreased by 45.99%, the MDA decreased by 58.77%, the LDH decreased by 71.61%, and the GSH increased by 206.60% (P<0.05). The expression of antioxidant enzyme-related genes including CuZnSOD, GCLC, GPx, and GSH also gradually returned to the normal level, all in a dose-dependent manner. Conclusion: The polyphenol of lotus seed epicarp protected against the oxidative damage induced by T-BHP in HepG2 cells. The mechanism might be related to the increase of intracellular antioxidant enzyme activity and the clearance of excessive ROS.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022070173polyphenol of lotus seed epicarphepg2anti-oxidative stressreactive oxygen speciestert-butylhydro-peroxide |
| spellingShingle | Yajun LU Ying LIU Yi WANG Wen HUANG Protective Effects of Polyphenol of Lotus Seed Epicarp on Oxidative Stress Damage Induced by T-BHP Shipin gongye ke-ji polyphenol of lotus seed epicarp hepg2 anti-oxidative stress reactive oxygen species tert-butylhydro-peroxide |
| title | Protective Effects of Polyphenol of Lotus Seed Epicarp on Oxidative Stress Damage Induced by T-BHP |
| title_full | Protective Effects of Polyphenol of Lotus Seed Epicarp on Oxidative Stress Damage Induced by T-BHP |
| title_fullStr | Protective Effects of Polyphenol of Lotus Seed Epicarp on Oxidative Stress Damage Induced by T-BHP |
| title_full_unstemmed | Protective Effects of Polyphenol of Lotus Seed Epicarp on Oxidative Stress Damage Induced by T-BHP |
| title_short | Protective Effects of Polyphenol of Lotus Seed Epicarp on Oxidative Stress Damage Induced by T-BHP |
| title_sort | protective effects of polyphenol of lotus seed epicarp on oxidative stress damage induced by t bhp |
| topic | polyphenol of lotus seed epicarp hepg2 anti-oxidative stress reactive oxygen species tert-butylhydro-peroxide |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022070173 |
| work_keys_str_mv | AT yajunlu protectiveeffectsofpolyphenoloflotusseedepicarponoxidativestressdamageinducedbytbhp AT yingliu protectiveeffectsofpolyphenoloflotusseedepicarponoxidativestressdamageinducedbytbhp AT yiwang protectiveeffectsofpolyphenoloflotusseedepicarponoxidativestressdamageinducedbytbhp AT wenhuang protectiveeffectsofpolyphenoloflotusseedepicarponoxidativestressdamageinducedbytbhp |