Transcriptome and Metabolome Analyses Revealed the Response Mechanism of Sugar Beet to Salt Stress of Different Durations
Salinity is one of the most serious threats to agriculture worldwide. Sugar beet is an important sugar-yielding crop and has a certain tolerance to salt; however, the genome-wide dynamic response to salt stress remains largely unknown in sugar beet. In the present study, physiological and transcript...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/17/9599 |
| _version_ | 1797495311364521984 |
|---|---|
| author | Jie Cui Junliang Li Cuihong Dai Liping Li |
| author_facet | Jie Cui Junliang Li Cuihong Dai Liping Li |
| author_sort | Jie Cui |
| collection | DOAJ |
| description | Salinity is one of the most serious threats to agriculture worldwide. Sugar beet is an important sugar-yielding crop and has a certain tolerance to salt; however, the genome-wide dynamic response to salt stress remains largely unknown in sugar beet. In the present study, physiological and transcriptome analyses of sugar beet leaves and roots were compared under salt stress at five time points. The results showed that different salt stresses influenced phenotypic characteristics, leaf relative water content and root activity in sugar beet. The contents of chlorophyll, malondialdehyde (MDA), the activities of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were also affected by different salt stresses. Compared with control plants, there were 7391 and 8729 differentially expressed genes (DEGs) in leaves and roots under salt stress, respectively. A total of 41 hub genes related to salt stress were identified by weighted gene co-expression network analysis (WGCNA) from DEGs, and a transcriptional regulatory network based on these genes was constructed. The expression pattern of hub genes under salt stress was confirmed by qRT-PCR. In addition, the metabolite of sugar beet was compared under salt stress for 24 h. A total of 157 and 157 differentially accumulated metabolites (DAMs) were identified in leaves and roots, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis further indicated that DEGs and DAMs act on the starch and sucrose metabolism, alpha-linolenic acid metabolism, phenylpropanoid biosynthesis and plant hormone signal transduction pathway. In this study, RNA-seq, WGCNA analysis and untargeted metabolomics were combined to investigate the transcriptional and metabolic changes of sugar beet during salt stress. The results provided new insights into the molecular mechanism of sugar beet response to salt stress, and also provided candidate genes for sugar beet improvement. |
| first_indexed | 2024-03-10T01:47:01Z |
| format | Article |
| id | doaj.art-42769ba727ba453ca4a2756a7ea6e474 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:47:01Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-42769ba727ba453ca4a2756a7ea6e4742023-11-23T13:13:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012317959910.3390/ijms23179599Transcriptome and Metabolome Analyses Revealed the Response Mechanism of Sugar Beet to Salt Stress of Different DurationsJie Cui0Junliang Li1Cuihong Dai2Liping Li3School of Medicine and Health, Harbin Institute of Technology, Harbin 150086, ChinaCollege of Life and Environmental Science, Wenzhou University, Wenzhou 325035, ChinaSchool of Medicine and Health, Harbin Institute of Technology, Harbin 150086, ChinaSchool of Medicine and Health, Harbin Institute of Technology, Harbin 150086, ChinaSalinity is one of the most serious threats to agriculture worldwide. Sugar beet is an important sugar-yielding crop and has a certain tolerance to salt; however, the genome-wide dynamic response to salt stress remains largely unknown in sugar beet. In the present study, physiological and transcriptome analyses of sugar beet leaves and roots were compared under salt stress at five time points. The results showed that different salt stresses influenced phenotypic characteristics, leaf relative water content and root activity in sugar beet. The contents of chlorophyll, malondialdehyde (MDA), the activities of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) were also affected by different salt stresses. Compared with control plants, there were 7391 and 8729 differentially expressed genes (DEGs) in leaves and roots under salt stress, respectively. A total of 41 hub genes related to salt stress were identified by weighted gene co-expression network analysis (WGCNA) from DEGs, and a transcriptional regulatory network based on these genes was constructed. The expression pattern of hub genes under salt stress was confirmed by qRT-PCR. In addition, the metabolite of sugar beet was compared under salt stress for 24 h. A total of 157 and 157 differentially accumulated metabolites (DAMs) were identified in leaves and roots, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis further indicated that DEGs and DAMs act on the starch and sucrose metabolism, alpha-linolenic acid metabolism, phenylpropanoid biosynthesis and plant hormone signal transduction pathway. In this study, RNA-seq, WGCNA analysis and untargeted metabolomics were combined to investigate the transcriptional and metabolic changes of sugar beet during salt stress. The results provided new insights into the molecular mechanism of sugar beet response to salt stress, and also provided candidate genes for sugar beet improvement.https://www.mdpi.com/1422-0067/23/17/9599RNA-sequntargeted metabolomicsWGCNAsalt stresssugar beet |
| spellingShingle | Jie Cui Junliang Li Cuihong Dai Liping Li Transcriptome and Metabolome Analyses Revealed the Response Mechanism of Sugar Beet to Salt Stress of Different Durations International Journal of Molecular Sciences RNA-seq untargeted metabolomics WGCNA salt stress sugar beet |
| title | Transcriptome and Metabolome Analyses Revealed the Response Mechanism of Sugar Beet to Salt Stress of Different Durations |
| title_full | Transcriptome and Metabolome Analyses Revealed the Response Mechanism of Sugar Beet to Salt Stress of Different Durations |
| title_fullStr | Transcriptome and Metabolome Analyses Revealed the Response Mechanism of Sugar Beet to Salt Stress of Different Durations |
| title_full_unstemmed | Transcriptome and Metabolome Analyses Revealed the Response Mechanism of Sugar Beet to Salt Stress of Different Durations |
| title_short | Transcriptome and Metabolome Analyses Revealed the Response Mechanism of Sugar Beet to Salt Stress of Different Durations |
| title_sort | transcriptome and metabolome analyses revealed the response mechanism of sugar beet to salt stress of different durations |
| topic | RNA-seq untargeted metabolomics WGCNA salt stress sugar beet |
| url | https://www.mdpi.com/1422-0067/23/17/9599 |
| work_keys_str_mv | AT jiecui transcriptomeandmetabolomeanalysesrevealedtheresponsemechanismofsugarbeettosaltstressofdifferentdurations AT junliangli transcriptomeandmetabolomeanalysesrevealedtheresponsemechanismofsugarbeettosaltstressofdifferentdurations AT cuihongdai transcriptomeandmetabolomeanalysesrevealedtheresponsemechanismofsugarbeettosaltstressofdifferentdurations AT lipingli transcriptomeandmetabolomeanalysesrevealedtheresponsemechanismofsugarbeettosaltstressofdifferentdurations |