Molecular detection of Coxiella burnetii from the formalin-fixed tissues of Q fever patients with acute hepatitis.
Serologic diagnosis is one of the most widely used diagnostic methods for Q fever, but the window period in antibody response of 2 to 3 weeks after symptom onset results in significant diagnostic delay. We investigated the diagnostic utility of Q fever PCR from formalin-fixed liver tissues in Q feve...
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Public Library of Science (PLoS)
2017-01-01
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author | Young-Rock Jang Yong Shin Choong Eun Jin Bonhan Koo Se Yoon Park Min-Chul Kim Taeeun Kim Yong Pil Chong Sang-Oh Lee Sang-Ho Choi Yang Soo Kim Jun Hee Woo Sung-Han Kim Eunsil Yu |
author_facet | Young-Rock Jang Yong Shin Choong Eun Jin Bonhan Koo Se Yoon Park Min-Chul Kim Taeeun Kim Yong Pil Chong Sang-Oh Lee Sang-Ho Choi Yang Soo Kim Jun Hee Woo Sung-Han Kim Eunsil Yu |
author_sort | Young-Rock Jang |
collection | DOAJ |
description | Serologic diagnosis is one of the most widely used diagnostic methods for Q fever, but the window period in antibody response of 2 to 3 weeks after symptom onset results in significant diagnostic delay. We investigated the diagnostic utility of Q fever PCR from formalin-fixed liver tissues in Q fever patients with acute hepatitis.We reviewed the clinical and laboratory data in patients with Q fever hepatitis who underwent liver biopsy during a 17-year period, and whose biopsied tissues were available. We also selected patients who revealed granuloma in liver biopsy and with no Q fever diagnosis within the last 3 years as control. Acute Q fever hepatitis was diagnosed if two or more of the following clinical, serologic, or histopathologic criteria were met: (1) an infectious hepatitis-like clinical feature such as fever (≥ 38°C) with elevated hepatic transaminase levels; (2) exhibition of a phase II immunoglobulin G (IgG) antibodies titer by IFA of ≥ 1:128 in single determination, or a four-fold or greater rise between two separate samples obtained two or more weeks apart; (3) histologic finding of biopsy tissue showing characteristic fibrin ring granuloma.A total of 11 patients with acute Q fever hepatitis were selected and analyzed. Of the 11 patients, 3 (27%) had exposure to zoonotic risk factors and 7 (63%) met the serologic criteria. Granulomas with either circumferential or radiating fibrin deposition were observed in 10 cases on liver biopsy and in 1 case on bone marrow biopsy. 8 (73%) revealed positive Coxiella burnetii PCR from their formalin-fixed liver tissues. In contrast, none of 10 patients with alternative diagnosis who had hepatic granuloma revealed positive C. burnetii PCR from their formalin-fixed liver tissues.Q fever PCR from formalin-fixed liver tissues appears to be a useful adjunct for diagnosing Q fever hepatitis. |
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language | English |
last_indexed | 2024-04-12T01:13:11Z |
publishDate | 2017-01-01 |
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spelling | doaj.art-42777c4a8b184a9a95e13dcd9e5e8cd12022-12-22T03:54:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01127e018023710.1371/journal.pone.0180237Molecular detection of Coxiella burnetii from the formalin-fixed tissues of Q fever patients with acute hepatitis.Young-Rock JangYong ShinChoong Eun JinBonhan KooSe Yoon ParkMin-Chul KimTaeeun KimYong Pil ChongSang-Oh LeeSang-Ho ChoiYang Soo KimJun Hee WooSung-Han KimEunsil YuSerologic diagnosis is one of the most widely used diagnostic methods for Q fever, but the window period in antibody response of 2 to 3 weeks after symptom onset results in significant diagnostic delay. We investigated the diagnostic utility of Q fever PCR from formalin-fixed liver tissues in Q fever patients with acute hepatitis.We reviewed the clinical and laboratory data in patients with Q fever hepatitis who underwent liver biopsy during a 17-year period, and whose biopsied tissues were available. We also selected patients who revealed granuloma in liver biopsy and with no Q fever diagnosis within the last 3 years as control. Acute Q fever hepatitis was diagnosed if two or more of the following clinical, serologic, or histopathologic criteria were met: (1) an infectious hepatitis-like clinical feature such as fever (≥ 38°C) with elevated hepatic transaminase levels; (2) exhibition of a phase II immunoglobulin G (IgG) antibodies titer by IFA of ≥ 1:128 in single determination, or a four-fold or greater rise between two separate samples obtained two or more weeks apart; (3) histologic finding of biopsy tissue showing characteristic fibrin ring granuloma.A total of 11 patients with acute Q fever hepatitis were selected and analyzed. Of the 11 patients, 3 (27%) had exposure to zoonotic risk factors and 7 (63%) met the serologic criteria. Granulomas with either circumferential or radiating fibrin deposition were observed in 10 cases on liver biopsy and in 1 case on bone marrow biopsy. 8 (73%) revealed positive Coxiella burnetii PCR from their formalin-fixed liver tissues. In contrast, none of 10 patients with alternative diagnosis who had hepatic granuloma revealed positive C. burnetii PCR from their formalin-fixed liver tissues.Q fever PCR from formalin-fixed liver tissues appears to be a useful adjunct for diagnosing Q fever hepatitis.http://europepmc.org/articles/PMC5495296?pdf=render |
spellingShingle | Young-Rock Jang Yong Shin Choong Eun Jin Bonhan Koo Se Yoon Park Min-Chul Kim Taeeun Kim Yong Pil Chong Sang-Oh Lee Sang-Ho Choi Yang Soo Kim Jun Hee Woo Sung-Han Kim Eunsil Yu Molecular detection of Coxiella burnetii from the formalin-fixed tissues of Q fever patients with acute hepatitis. PLoS ONE |
title | Molecular detection of Coxiella burnetii from the formalin-fixed tissues of Q fever patients with acute hepatitis. |
title_full | Molecular detection of Coxiella burnetii from the formalin-fixed tissues of Q fever patients with acute hepatitis. |
title_fullStr | Molecular detection of Coxiella burnetii from the formalin-fixed tissues of Q fever patients with acute hepatitis. |
title_full_unstemmed | Molecular detection of Coxiella burnetii from the formalin-fixed tissues of Q fever patients with acute hepatitis. |
title_short | Molecular detection of Coxiella burnetii from the formalin-fixed tissues of Q fever patients with acute hepatitis. |
title_sort | molecular detection of coxiella burnetii from the formalin fixed tissues of q fever patients with acute hepatitis |
url | http://europepmc.org/articles/PMC5495296?pdf=render |
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