Applying real-time monitoring of circadian oscillations in adult mouse brain slices to study communications between brain regions
Summary: This protocol combines a protective cutting method to prepare various brain slices from adult mice and real-time monitoring of circadian oscillations to measure circadian rhythmicity in various brain slices. This protocol can be applied to studies of how brain damages affect local circadian...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721001234 |
| _version_ | 1818669215965511680 |
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| author | Suihong Huang Qingqing Lu Ming Ho Choi Xuebing Zhang Jin Young Kim |
| author_facet | Suihong Huang Qingqing Lu Ming Ho Choi Xuebing Zhang Jin Young Kim |
| author_sort | Suihong Huang |
| collection | DOAJ |
| description | Summary: This protocol combines a protective cutting method to prepare various brain slices from adult mice and real-time monitoring of circadian oscillations to measure circadian rhythmicity in various brain slices. This protocol can be applied to studies of how brain damages affect local circadian clocks and subsequent circadian variations in nearby areas. Further functional analyses with in vivo systems will determine whether these circadian variations are detrimental or beneficial to the brain.For complete details on the use and execution of this protocol, please refer to Huang et al. (2020). |
| first_indexed | 2024-12-17T06:48:41Z |
| format | Article |
| id | doaj.art-4281045a242f4286953faa1bf9ae29c9 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-17T06:48:41Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-4281045a242f4286953faa1bf9ae29c92022-12-21T21:59:39ZengElsevierSTAR Protocols2666-16672021-06-0122100416Applying real-time monitoring of circadian oscillations in adult mouse brain slices to study communications between brain regionsSuihong Huang0Qingqing Lu1Ming Ho Choi2Xuebing Zhang3Jin Young Kim4Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong, ChinaDepartment of Biomedical Sciences, City University of Hong Kong, Hong Kong, ChinaDepartment of Biomedical Sciences, City University of Hong Kong, Hong Kong, ChinaDepartment of Biomedical Sciences, City University of Hong Kong, Hong Kong, ChinaDepartment of Biomedical Sciences, City University of Hong Kong, Hong Kong, China; Shenzhen Research Institute, City University of Hong Kong, Shenzhen, China; Corresponding authorSummary: This protocol combines a protective cutting method to prepare various brain slices from adult mice and real-time monitoring of circadian oscillations to measure circadian rhythmicity in various brain slices. This protocol can be applied to studies of how brain damages affect local circadian clocks and subsequent circadian variations in nearby areas. Further functional analyses with in vivo systems will determine whether these circadian variations are detrimental or beneficial to the brain.For complete details on the use and execution of this protocol, please refer to Huang et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721001234Cell BiologyModel OrganismsNeuroscience |
| spellingShingle | Suihong Huang Qingqing Lu Ming Ho Choi Xuebing Zhang Jin Young Kim Applying real-time monitoring of circadian oscillations in adult mouse brain slices to study communications between brain regions STAR Protocols Cell Biology Model Organisms Neuroscience |
| title | Applying real-time monitoring of circadian oscillations in adult mouse brain slices to study communications between brain regions |
| title_full | Applying real-time monitoring of circadian oscillations in adult mouse brain slices to study communications between brain regions |
| title_fullStr | Applying real-time monitoring of circadian oscillations in adult mouse brain slices to study communications between brain regions |
| title_full_unstemmed | Applying real-time monitoring of circadian oscillations in adult mouse brain slices to study communications between brain regions |
| title_short | Applying real-time monitoring of circadian oscillations in adult mouse brain slices to study communications between brain regions |
| title_sort | applying real time monitoring of circadian oscillations in adult mouse brain slices to study communications between brain regions |
| topic | Cell Biology Model Organisms Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721001234 |
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