Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs
Abstract The epidemic of obesity and diabetes has markedly spurred the research interest in adipocyte biology. Brown adipocytes are specialized for energy expenditure and of therapeutic interest for treatment of metabolic diseases, but how brown adipocytes are distinguished from white adipocytes at...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-06077-3 |
| _version_ | 1828977432227479552 |
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| author | David W. Reid Dan Xu Peng Chen Hongyuan Yang Lei Sun |
| author_facet | David W. Reid Dan Xu Peng Chen Hongyuan Yang Lei Sun |
| author_sort | David W. Reid |
| collection | DOAJ |
| description | Abstract The epidemic of obesity and diabetes has markedly spurred the research interest in adipocyte biology. Brown adipocytes are specialized for energy expenditure and of therapeutic interest for treatment of metabolic diseases, but how brown adipocytes are distinguished from white adipocytes at the level of translational regulation remains poorly understood. To systemically determine the translational control of gene expression in adipose tissue, we performed ribosome profiling and RNA-seq in parallel to depict the translatome and transcriptome changes during primary brown and white adipogenesis, and between brown and white adipose tissue. The most prominent layer of translational regulation was the increased translation efficiency of genes encoding mitochondria components in brown adipocytes relative to white. Systemic analysis of the regulatory interactions between microRNAs and their targets revealed that microRNAs were more active in repressing targets’ mRNA abundance and translation in brown fat. Together, our data comprehensively delineated a landscape integrating transcriptome and translatome in adipose tissue. |
| first_indexed | 2024-12-14T14:57:30Z |
| format | Article |
| id | doaj.art-42814b23c8b94a7fb4c6fb81551195e8 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T14:57:30Z |
| publishDate | 2017-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-42814b23c8b94a7fb4c6fb81551195e82022-12-21T22:56:57ZengNature PortfolioScientific Reports2045-23222017-07-017111110.1038/s41598-017-06077-3Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAsDavid W. Reid0Dan Xu1Peng Chen2Hongyuan Yang3Lei Sun4Cardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical SchoolCardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical SchoolDivision of Bioengineering, Nanyang Technological UniversitySchool of Biotechnology and Biomolecular Sciences, University of New South WalesCardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical SchoolAbstract The epidemic of obesity and diabetes has markedly spurred the research interest in adipocyte biology. Brown adipocytes are specialized for energy expenditure and of therapeutic interest for treatment of metabolic diseases, but how brown adipocytes are distinguished from white adipocytes at the level of translational regulation remains poorly understood. To systemically determine the translational control of gene expression in adipose tissue, we performed ribosome profiling and RNA-seq in parallel to depict the translatome and transcriptome changes during primary brown and white adipogenesis, and between brown and white adipose tissue. The most prominent layer of translational regulation was the increased translation efficiency of genes encoding mitochondria components in brown adipocytes relative to white. Systemic analysis of the regulatory interactions between microRNAs and their targets revealed that microRNAs were more active in repressing targets’ mRNA abundance and translation in brown fat. Together, our data comprehensively delineated a landscape integrating transcriptome and translatome in adipose tissue.https://doi.org/10.1038/s41598-017-06077-3 |
| spellingShingle | David W. Reid Dan Xu Peng Chen Hongyuan Yang Lei Sun Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs Scientific Reports |
| title | Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs |
| title_full | Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs |
| title_fullStr | Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs |
| title_full_unstemmed | Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs |
| title_short | Integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by microRNAs |
| title_sort | integrative analyses of translatome and transcriptome reveal important translational controls in brown and white adipose regulated by micrornas |
| url | https://doi.org/10.1038/s41598-017-06077-3 |
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