Screening and Optimization of Fermentation Medium for <i>Bacillus velezensis</i> BP-1 and Its Biocontrol Effects against <i>Peyronellaea arachidicola</i>
In the submerged fermentation process, the introduction of novel nutrient media as sources of carbon and nitrogen can enhance antifungal activity. In this study, we used a submerged fermentation process to find the optimal media for the <i>Bacillus velezensis</i> BP-1 strain to grow in,...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/13/8/4653 |
| _version_ | 1797606621327654912 |
|---|---|
| author | Taswar Ahsan Chunhao Liang Shuyi Yu Xue Pei Jinhui Xie Ying Lin Xiaozhou Liu Muhammad Umair Chaoqun Zang |
| author_facet | Taswar Ahsan Chunhao Liang Shuyi Yu Xue Pei Jinhui Xie Ying Lin Xiaozhou Liu Muhammad Umair Chaoqun Zang |
| author_sort | Taswar Ahsan |
| collection | DOAJ |
| description | In the submerged fermentation process, the introduction of novel nutrient media as sources of carbon and nitrogen can enhance antifungal activity. In this study, we used a submerged fermentation process to find the optimal media for the <i>Bacillus velezensis</i> BP-1 strain to grow in, and that would boost its antifungal activity against <i>Peyronellaea arachidicola</i>. By using a single-factor test and central composite design (CCD) in the response surface methodology, the optimal fermentation medium for the <i>B. velezensis</i> BP-1 strain was identified. The antagonistic potential of <i>B. velezensis</i> BP-1 was assayed against the <i>P. arachidicola</i> fungus to manage web blotch disease in peanut plant leaves. The LB medium was screened as the best medium, with a maximum antifungal activity of 90% in comparison to the other mediums. Semolina flour as the carbon source, peanut root extract as the nitrogen source, and magnesium sulfate as the inorganic salt were selected as the best nutrient components in comparison to the others. The response surface methodology was optimized by using 15 g/L of semolina flour as the carbon source, 13.68 g/L of peanut root extract as the nitrogen source, and 0.50 g/L of magnesium sulfate as the inorganic salt, achieving 90% inhibition of <i>P. arachidicola</i>. The in vitro bioassays showed that the optimized fermentation broth of <i>B. velezensis</i> BP-1 had significant antifungal activity, with an inhibition rate of 88.34% against <i>P. arachidicola</i>. In the pot experiments on disease control, the management effects revealed that the pre-inoculation spray of the <i>B. velezensis</i> BP-1 broth had significant efficiency (96%) when compared to the post-inoculation spray of the <i>B. velezensis</i> BP-1 broth. These findings suggest that the optimized fermentation broth of the <i>B. velezensis</i> BP-1 strain had strong antifungal activity. This could be a potent biocontrol tool for aiding in the early disease management of web blotch in peanut plants. |
| first_indexed | 2024-03-11T05:17:48Z |
| format | Article |
| id | doaj.art-428187871a3c4eb9a1ab3029c59b07c8 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T05:17:48Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-428187871a3c4eb9a1ab3029c59b07c82023-11-17T18:07:13ZengMDPI AGApplied Sciences2076-34172023-04-01138465310.3390/app13084653Screening and Optimization of Fermentation Medium for <i>Bacillus velezensis</i> BP-1 and Its Biocontrol Effects against <i>Peyronellaea arachidicola</i>Taswar Ahsan0Chunhao Liang1Shuyi Yu2Xue Pei3Jinhui Xie4Ying Lin5Xiaozhou Liu6Muhammad Umair7Chaoqun Zang8Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161, ChinaInstitute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161, ChinaInstitute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161, ChinaInstitute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161, ChinaInstitute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161, ChinaInstitute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161, ChinaInstitute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161, ChinaCollege of Life Science, Zhejiang Normal University, Jinhua 321004, ChinaInstitute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161, ChinaIn the submerged fermentation process, the introduction of novel nutrient media as sources of carbon and nitrogen can enhance antifungal activity. In this study, we used a submerged fermentation process to find the optimal media for the <i>Bacillus velezensis</i> BP-1 strain to grow in, and that would boost its antifungal activity against <i>Peyronellaea arachidicola</i>. By using a single-factor test and central composite design (CCD) in the response surface methodology, the optimal fermentation medium for the <i>B. velezensis</i> BP-1 strain was identified. The antagonistic potential of <i>B. velezensis</i> BP-1 was assayed against the <i>P. arachidicola</i> fungus to manage web blotch disease in peanut plant leaves. The LB medium was screened as the best medium, with a maximum antifungal activity of 90% in comparison to the other mediums. Semolina flour as the carbon source, peanut root extract as the nitrogen source, and magnesium sulfate as the inorganic salt were selected as the best nutrient components in comparison to the others. The response surface methodology was optimized by using 15 g/L of semolina flour as the carbon source, 13.68 g/L of peanut root extract as the nitrogen source, and 0.50 g/L of magnesium sulfate as the inorganic salt, achieving 90% inhibition of <i>P. arachidicola</i>. The in vitro bioassays showed that the optimized fermentation broth of <i>B. velezensis</i> BP-1 had significant antifungal activity, with an inhibition rate of 88.34% against <i>P. arachidicola</i>. In the pot experiments on disease control, the management effects revealed that the pre-inoculation spray of the <i>B. velezensis</i> BP-1 broth had significant efficiency (96%) when compared to the post-inoculation spray of the <i>B. velezensis</i> BP-1 broth. These findings suggest that the optimized fermentation broth of the <i>B. velezensis</i> BP-1 strain had strong antifungal activity. This could be a potent biocontrol tool for aiding in the early disease management of web blotch in peanut plants.https://www.mdpi.com/2076-3417/13/8/4653response surface methodologyoptimization of mediumpeanut web blotch<i>Bacillus velezensis</i> BP-1<i>Peyronellaea arachidicola</i>biocontrol |
| spellingShingle | Taswar Ahsan Chunhao Liang Shuyi Yu Xue Pei Jinhui Xie Ying Lin Xiaozhou Liu Muhammad Umair Chaoqun Zang Screening and Optimization of Fermentation Medium for <i>Bacillus velezensis</i> BP-1 and Its Biocontrol Effects against <i>Peyronellaea arachidicola</i> Applied Sciences response surface methodology optimization of medium peanut web blotch <i>Bacillus velezensis</i> BP-1 <i>Peyronellaea arachidicola</i> biocontrol |
| title | Screening and Optimization of Fermentation Medium for <i>Bacillus velezensis</i> BP-1 and Its Biocontrol Effects against <i>Peyronellaea arachidicola</i> |
| title_full | Screening and Optimization of Fermentation Medium for <i>Bacillus velezensis</i> BP-1 and Its Biocontrol Effects against <i>Peyronellaea arachidicola</i> |
| title_fullStr | Screening and Optimization of Fermentation Medium for <i>Bacillus velezensis</i> BP-1 and Its Biocontrol Effects against <i>Peyronellaea arachidicola</i> |
| title_full_unstemmed | Screening and Optimization of Fermentation Medium for <i>Bacillus velezensis</i> BP-1 and Its Biocontrol Effects against <i>Peyronellaea arachidicola</i> |
| title_short | Screening and Optimization of Fermentation Medium for <i>Bacillus velezensis</i> BP-1 and Its Biocontrol Effects against <i>Peyronellaea arachidicola</i> |
| title_sort | screening and optimization of fermentation medium for i bacillus velezensis i bp 1 and its biocontrol effects against i peyronellaea arachidicola i |
| topic | response surface methodology optimization of medium peanut web blotch <i>Bacillus velezensis</i> BP-1 <i>Peyronellaea arachidicola</i> biocontrol |
| url | https://www.mdpi.com/2076-3417/13/8/4653 |
| work_keys_str_mv | AT taswarahsan screeningandoptimizationoffermentationmediumforibacillusvelezensisibp1anditsbiocontroleffectsagainstipeyronellaeaarachidicolai AT chunhaoliang screeningandoptimizationoffermentationmediumforibacillusvelezensisibp1anditsbiocontroleffectsagainstipeyronellaeaarachidicolai AT shuyiyu screeningandoptimizationoffermentationmediumforibacillusvelezensisibp1anditsbiocontroleffectsagainstipeyronellaeaarachidicolai AT xuepei screeningandoptimizationoffermentationmediumforibacillusvelezensisibp1anditsbiocontroleffectsagainstipeyronellaeaarachidicolai AT jinhuixie screeningandoptimizationoffermentationmediumforibacillusvelezensisibp1anditsbiocontroleffectsagainstipeyronellaeaarachidicolai AT yinglin screeningandoptimizationoffermentationmediumforibacillusvelezensisibp1anditsbiocontroleffectsagainstipeyronellaeaarachidicolai AT xiaozhouliu screeningandoptimizationoffermentationmediumforibacillusvelezensisibp1anditsbiocontroleffectsagainstipeyronellaeaarachidicolai AT muhammadumair screeningandoptimizationoffermentationmediumforibacillusvelezensisibp1anditsbiocontroleffectsagainstipeyronellaeaarachidicolai AT chaoqunzang screeningandoptimizationoffermentationmediumforibacillusvelezensisibp1anditsbiocontroleffectsagainstipeyronellaeaarachidicolai |