Adiponectin Modulates Smooth Muscle Cell Morpho-Functional Properties in Murine Gastric Fundus via Sphingosine Kinase 2 Activation
Adipokines are peptide hormones produced by the adipose tissue involved in several biological functions. Among adipokines, adiponectin (ADPN) has antidiabetic and anti-inflammatory properties. It can also modulate food intake at central and peripheral levels, acting on hypothalamus and facilitating...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-08-01
|
| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/13/9/1812 |
| _version_ | 1797579154444517376 |
|---|---|
| author | Rachele Garella Caterina Bernacchioni Flaminia Chellini Alessia Tani Francesco Palmieri Martina Parigi Daniele Guasti Emanuele Cassioli Giovanni Castellini Valdo Ricca Daniele Bani Chiara Sassoli Chiara Donati Roberta Squecco |
| author_facet | Rachele Garella Caterina Bernacchioni Flaminia Chellini Alessia Tani Francesco Palmieri Martina Parigi Daniele Guasti Emanuele Cassioli Giovanni Castellini Valdo Ricca Daniele Bani Chiara Sassoli Chiara Donati Roberta Squecco |
| author_sort | Rachele Garella |
| collection | DOAJ |
| description | Adipokines are peptide hormones produced by the adipose tissue involved in several biological functions. Among adipokines, adiponectin (ADPN) has antidiabetic and anti-inflammatory properties. It can also modulate food intake at central and peripheral levels, acting on hypothalamus and facilitating gastric relaxation. ADPN exerts its action interacting with two distinct membrane receptors and triggering some well-defined signaling cascades. The ceramidase activity of ADPN receptor has been reported in many tissues: it converts ceramide into sphingosine. In turn, sphingosine kinase (SK) phosphorylates it into sphingosine-1 phosphate (S1P), a crucial mediator of many cellular processes including contractility. Using a multidisciplinary approach that combined biochemical, electrophysiological and morphological investigations, we explored for the first time the possible role of S1P metabolism in mediating ADPN effects on the murine gastric fundus muscle layer. By using a specific pharmacological inhibitor of SK2, we showed that ADPN affects smooth muscle cell membrane properties and contractile machinery via SK2 activation in gastric fundus, adding a piece of knowledge to the action mechanisms of this hormone. These findings help to identify ADPN and its receptors as new therapeutic targets or as possible prognostic markers for diseases with altered energy balance and for pathologies with fat mass content alterations. |
| first_indexed | 2024-03-10T22:32:50Z |
| format | Article |
| id | doaj.art-42854416c8504ddba737f03616cbcb13 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T22:32:50Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-42854416c8504ddba737f03616cbcb132023-11-19T11:36:39ZengMDPI AGLife2075-17292023-08-01139181210.3390/life13091812Adiponectin Modulates Smooth Muscle Cell Morpho-Functional Properties in Murine Gastric Fundus via Sphingosine Kinase 2 ActivationRachele Garella0Caterina Bernacchioni1Flaminia Chellini2Alessia Tani3Francesco Palmieri4Martina Parigi5Daniele Guasti6Emanuele Cassioli7Giovanni Castellini8Valdo Ricca9Daniele Bani10Chiara Sassoli11Chiara Donati12Roberta Squecco13Department of Experimental and Clinical Medicine, Section of Physiological Sciences, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, Section of Anatomy and Histology, Imaging Platform, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, Section of Anatomy and Histology, Imaging Platform, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, Section of Physiological Sciences, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, Section of Anatomy and Histology, Imaging Platform, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, Section of Anatomy and Histology, Imaging Platform, University of Florence, 50134 Florence, ItalyPsychiatry Unit, Department of Health Sciences, University of Florence, 50134 Florence, ItalyPsychiatry Unit, Department of Health Sciences, University of Florence, 50134 Florence, ItalyPsychiatry Unit, Department of Health Sciences, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, Section of Anatomy and Histology, Imaging Platform, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, Section of Anatomy and Histology, Imaging Platform, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, Section of Physiological Sciences, University of Florence, 50134 Florence, ItalyAdipokines are peptide hormones produced by the adipose tissue involved in several biological functions. Among adipokines, adiponectin (ADPN) has antidiabetic and anti-inflammatory properties. It can also modulate food intake at central and peripheral levels, acting on hypothalamus and facilitating gastric relaxation. ADPN exerts its action interacting with two distinct membrane receptors and triggering some well-defined signaling cascades. The ceramidase activity of ADPN receptor has been reported in many tissues: it converts ceramide into sphingosine. In turn, sphingosine kinase (SK) phosphorylates it into sphingosine-1 phosphate (S1P), a crucial mediator of many cellular processes including contractility. Using a multidisciplinary approach that combined biochemical, electrophysiological and morphological investigations, we explored for the first time the possible role of S1P metabolism in mediating ADPN effects on the murine gastric fundus muscle layer. By using a specific pharmacological inhibitor of SK2, we showed that ADPN affects smooth muscle cell membrane properties and contractile machinery via SK2 activation in gastric fundus, adding a piece of knowledge to the action mechanisms of this hormone. These findings help to identify ADPN and its receptors as new therapeutic targets or as possible prognostic markers for diseases with altered energy balance and for pathologies with fat mass content alterations.https://www.mdpi.com/2075-1729/13/9/1812adiponectinsphingosine kinasesmooth musclegastric fundusmorphologymembrane properties |
| spellingShingle | Rachele Garella Caterina Bernacchioni Flaminia Chellini Alessia Tani Francesco Palmieri Martina Parigi Daniele Guasti Emanuele Cassioli Giovanni Castellini Valdo Ricca Daniele Bani Chiara Sassoli Chiara Donati Roberta Squecco Adiponectin Modulates Smooth Muscle Cell Morpho-Functional Properties in Murine Gastric Fundus via Sphingosine Kinase 2 Activation Life adiponectin sphingosine kinase smooth muscle gastric fundus morphology membrane properties |
| title | Adiponectin Modulates Smooth Muscle Cell Morpho-Functional Properties in Murine Gastric Fundus via Sphingosine Kinase 2 Activation |
| title_full | Adiponectin Modulates Smooth Muscle Cell Morpho-Functional Properties in Murine Gastric Fundus via Sphingosine Kinase 2 Activation |
| title_fullStr | Adiponectin Modulates Smooth Muscle Cell Morpho-Functional Properties in Murine Gastric Fundus via Sphingosine Kinase 2 Activation |
| title_full_unstemmed | Adiponectin Modulates Smooth Muscle Cell Morpho-Functional Properties in Murine Gastric Fundus via Sphingosine Kinase 2 Activation |
| title_short | Adiponectin Modulates Smooth Muscle Cell Morpho-Functional Properties in Murine Gastric Fundus via Sphingosine Kinase 2 Activation |
| title_sort | adiponectin modulates smooth muscle cell morpho functional properties in murine gastric fundus via sphingosine kinase 2 activation |
| topic | adiponectin sphingosine kinase smooth muscle gastric fundus morphology membrane properties |
| url | https://www.mdpi.com/2075-1729/13/9/1812 |
| work_keys_str_mv | AT rachelegarella adiponectinmodulatessmoothmusclecellmorphofunctionalpropertiesinmurinegastricfundusviasphingosinekinase2activation AT caterinabernacchioni adiponectinmodulatessmoothmusclecellmorphofunctionalpropertiesinmurinegastricfundusviasphingosinekinase2activation AT flaminiachellini adiponectinmodulatessmoothmusclecellmorphofunctionalpropertiesinmurinegastricfundusviasphingosinekinase2activation AT alessiatani adiponectinmodulatessmoothmusclecellmorphofunctionalpropertiesinmurinegastricfundusviasphingosinekinase2activation AT francescopalmieri adiponectinmodulatessmoothmusclecellmorphofunctionalpropertiesinmurinegastricfundusviasphingosinekinase2activation AT martinaparigi adiponectinmodulatessmoothmusclecellmorphofunctionalpropertiesinmurinegastricfundusviasphingosinekinase2activation AT danieleguasti adiponectinmodulatessmoothmusclecellmorphofunctionalpropertiesinmurinegastricfundusviasphingosinekinase2activation AT emanuelecassioli adiponectinmodulatessmoothmusclecellmorphofunctionalpropertiesinmurinegastricfundusviasphingosinekinase2activation AT giovannicastellini adiponectinmodulatessmoothmusclecellmorphofunctionalpropertiesinmurinegastricfundusviasphingosinekinase2activation AT valdoricca adiponectinmodulatessmoothmusclecellmorphofunctionalpropertiesinmurinegastricfundusviasphingosinekinase2activation AT danielebani adiponectinmodulatessmoothmusclecellmorphofunctionalpropertiesinmurinegastricfundusviasphingosinekinase2activation AT chiarasassoli adiponectinmodulatessmoothmusclecellmorphofunctionalpropertiesinmurinegastricfundusviasphingosinekinase2activation AT chiaradonati adiponectinmodulatessmoothmusclecellmorphofunctionalpropertiesinmurinegastricfundusviasphingosinekinase2activation AT robertasquecco adiponectinmodulatessmoothmusclecellmorphofunctionalpropertiesinmurinegastricfundusviasphingosinekinase2activation |