Changes in intestinal microbiota in postmenopausal oestrogen receptor-positive breast cancer patients treated with (neo)adjuvant chemotherapy
Abstract This clinical study explored the associations between the intestinal microbiota, chemotherapy toxicity, and treatment response in postmenopausal oestrogen receptor positive breast cancer patients.Oestrogen receptor positive postmenopausal breast cancer patients were prospectively enroled in...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-07-01
|
| Series: | npj Breast Cancer |
| Online Access: | https://doi.org/10.1038/s41523-022-00455-5 |
| _version_ | 1827775564822347776 |
|---|---|
| author | Romy Aarnoutse Janine Ziemons Lars E. Hillege Judith de Vos-Geelen Maaike de Boer Saskia M. P. Bisschop Birgit E. P. J. Vriens Jeroen Vincent Agnes J. van de Wouw Giang N. Le Koen Venema Sander S. Rensen John Penders Marjolein L. Smidt |
| author_facet | Romy Aarnoutse Janine Ziemons Lars E. Hillege Judith de Vos-Geelen Maaike de Boer Saskia M. P. Bisschop Birgit E. P. J. Vriens Jeroen Vincent Agnes J. van de Wouw Giang N. Le Koen Venema Sander S. Rensen John Penders Marjolein L. Smidt |
| author_sort | Romy Aarnoutse |
| collection | DOAJ |
| description | Abstract This clinical study explored the associations between the intestinal microbiota, chemotherapy toxicity, and treatment response in postmenopausal oestrogen receptor positive breast cancer patients.Oestrogen receptor positive postmenopausal breast cancer patients were prospectively enroled in a multicentre cohort study and treated with 4 cycles of (neo)adjuvant adriamycin, cyclophosphamide (AC) followed by 4 cycles of docetaxel (D). Patients collected a faecal sample and completed a questionnaire before treatment, during AC, during D, and after completing AC-D. Chemotherapy toxicity and tumour response were determined. Intestinal microbiota was analysed by amplicon sequencing of the 16 S rRNA V4 gene-region. In total, 44 patients, including 18 neoadjuvant patients, were included, and 153 faecal samples were collected before AC-D (n = 44), during AC (n = 43), during D (n = 29), and after AC-D treatment (n = 37), 28 participants provided all four samples. In the whole group, observed species richness reduced during treatment (p = 0.042). The abundance of Proteobacteria, unclassified Enterobacterales, Lactobacillus, Ruminococcaceae NK4A214 group, Marvinbryantia, Christensenellaceae R7 group, and Ruminococcaceae UCG-005 changed significantly over time. Patients with any grade diarrhoea during docetaxel treatment had a significantly lower observed species richness compared to patients without diarrhoea. In the small group neoadjuvant treated patients, pathologic response was unrelated to baseline intestinal microbiota richness, diversity and composition. While the baseline microbiota was not predictive for pathologic response in a rather small group of neoadjuvant treated patients in our study, subsequent shifts in microbial richness, as well as the abundance of specific bacterial taxa, were observed during AC-D treatment in the whole group and the neoadjuvant group. |
| first_indexed | 2024-03-11T13:52:10Z |
| format | Article |
| id | doaj.art-428760b3d68e44538ecc8341fa83aaa3 |
| institution | Directory Open Access Journal |
| issn | 2374-4677 |
| language | English |
| last_indexed | 2024-03-11T13:52:10Z |
| publishDate | 2022-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Breast Cancer |
| spelling | doaj.art-428760b3d68e44538ecc8341fa83aaa32023-11-02T08:22:18ZengNature Portfolionpj Breast Cancer2374-46772022-07-018111310.1038/s41523-022-00455-5Changes in intestinal microbiota in postmenopausal oestrogen receptor-positive breast cancer patients treated with (neo)adjuvant chemotherapyRomy Aarnoutse0Janine Ziemons1Lars E. Hillege2Judith de Vos-Geelen3Maaike de Boer4Saskia M. P. Bisschop5Birgit E. P. J. Vriens6Jeroen Vincent7Agnes J. van de Wouw8Giang N. Le9Koen Venema10Sander S. Rensen11John Penders12Marjolein L. Smidt13GROW - School for Oncology and Developmental Biology, Maastricht UniversityGROW - School for Oncology and Developmental Biology, Maastricht UniversityGROW - School for Oncology and Developmental Biology, Maastricht UniversityGROW - School for Oncology and Developmental Biology, Maastricht UniversityGROW - School for Oncology and Developmental Biology, Maastricht UniversityDepartment of Surgery, Maastricht University Medical CentreDepartment of Medical Oncology, Catharina HospitalDepartment of Medical Oncology, Elkerliek HospitalDepartment of Medical Oncology, VieCuri Medical CentreDepartment of Medical Microbiology, Maastricht University Medical CentreNUTRIM - School of Nutrition and Translational research In Metabolism, Maastricht UniversityDepartment of Surgery, Maastricht University Medical CentreDepartment of Medical Microbiology, Maastricht University Medical CentreGROW - School for Oncology and Developmental Biology, Maastricht UniversityAbstract This clinical study explored the associations between the intestinal microbiota, chemotherapy toxicity, and treatment response in postmenopausal oestrogen receptor positive breast cancer patients.Oestrogen receptor positive postmenopausal breast cancer patients were prospectively enroled in a multicentre cohort study and treated with 4 cycles of (neo)adjuvant adriamycin, cyclophosphamide (AC) followed by 4 cycles of docetaxel (D). Patients collected a faecal sample and completed a questionnaire before treatment, during AC, during D, and after completing AC-D. Chemotherapy toxicity and tumour response were determined. Intestinal microbiota was analysed by amplicon sequencing of the 16 S rRNA V4 gene-region. In total, 44 patients, including 18 neoadjuvant patients, were included, and 153 faecal samples were collected before AC-D (n = 44), during AC (n = 43), during D (n = 29), and after AC-D treatment (n = 37), 28 participants provided all four samples. In the whole group, observed species richness reduced during treatment (p = 0.042). The abundance of Proteobacteria, unclassified Enterobacterales, Lactobacillus, Ruminococcaceae NK4A214 group, Marvinbryantia, Christensenellaceae R7 group, and Ruminococcaceae UCG-005 changed significantly over time. Patients with any grade diarrhoea during docetaxel treatment had a significantly lower observed species richness compared to patients without diarrhoea. In the small group neoadjuvant treated patients, pathologic response was unrelated to baseline intestinal microbiota richness, diversity and composition. While the baseline microbiota was not predictive for pathologic response in a rather small group of neoadjuvant treated patients in our study, subsequent shifts in microbial richness, as well as the abundance of specific bacterial taxa, were observed during AC-D treatment in the whole group and the neoadjuvant group.https://doi.org/10.1038/s41523-022-00455-5 |
| spellingShingle | Romy Aarnoutse Janine Ziemons Lars E. Hillege Judith de Vos-Geelen Maaike de Boer Saskia M. P. Bisschop Birgit E. P. J. Vriens Jeroen Vincent Agnes J. van de Wouw Giang N. Le Koen Venema Sander S. Rensen John Penders Marjolein L. Smidt Changes in intestinal microbiota in postmenopausal oestrogen receptor-positive breast cancer patients treated with (neo)adjuvant chemotherapy npj Breast Cancer |
| title | Changes in intestinal microbiota in postmenopausal oestrogen receptor-positive breast cancer patients treated with (neo)adjuvant chemotherapy |
| title_full | Changes in intestinal microbiota in postmenopausal oestrogen receptor-positive breast cancer patients treated with (neo)adjuvant chemotherapy |
| title_fullStr | Changes in intestinal microbiota in postmenopausal oestrogen receptor-positive breast cancer patients treated with (neo)adjuvant chemotherapy |
| title_full_unstemmed | Changes in intestinal microbiota in postmenopausal oestrogen receptor-positive breast cancer patients treated with (neo)adjuvant chemotherapy |
| title_short | Changes in intestinal microbiota in postmenopausal oestrogen receptor-positive breast cancer patients treated with (neo)adjuvant chemotherapy |
| title_sort | changes in intestinal microbiota in postmenopausal oestrogen receptor positive breast cancer patients treated with neo adjuvant chemotherapy |
| url | https://doi.org/10.1038/s41523-022-00455-5 |
| work_keys_str_mv | AT romyaarnoutse changesinintestinalmicrobiotainpostmenopausaloestrogenreceptorpositivebreastcancerpatientstreatedwithneoadjuvantchemotherapy AT janineziemons changesinintestinalmicrobiotainpostmenopausaloestrogenreceptorpositivebreastcancerpatientstreatedwithneoadjuvantchemotherapy AT larsehillege changesinintestinalmicrobiotainpostmenopausaloestrogenreceptorpositivebreastcancerpatientstreatedwithneoadjuvantchemotherapy AT judithdevosgeelen changesinintestinalmicrobiotainpostmenopausaloestrogenreceptorpositivebreastcancerpatientstreatedwithneoadjuvantchemotherapy AT maaikedeboer changesinintestinalmicrobiotainpostmenopausaloestrogenreceptorpositivebreastcancerpatientstreatedwithneoadjuvantchemotherapy AT saskiampbisschop changesinintestinalmicrobiotainpostmenopausaloestrogenreceptorpositivebreastcancerpatientstreatedwithneoadjuvantchemotherapy AT birgitepjvriens changesinintestinalmicrobiotainpostmenopausaloestrogenreceptorpositivebreastcancerpatientstreatedwithneoadjuvantchemotherapy AT jeroenvincent changesinintestinalmicrobiotainpostmenopausaloestrogenreceptorpositivebreastcancerpatientstreatedwithneoadjuvantchemotherapy AT agnesjvandewouw changesinintestinalmicrobiotainpostmenopausaloestrogenreceptorpositivebreastcancerpatientstreatedwithneoadjuvantchemotherapy AT giangnle changesinintestinalmicrobiotainpostmenopausaloestrogenreceptorpositivebreastcancerpatientstreatedwithneoadjuvantchemotherapy AT koenvenema changesinintestinalmicrobiotainpostmenopausaloestrogenreceptorpositivebreastcancerpatientstreatedwithneoadjuvantchemotherapy AT sandersrensen changesinintestinalmicrobiotainpostmenopausaloestrogenreceptorpositivebreastcancerpatientstreatedwithneoadjuvantchemotherapy AT johnpenders changesinintestinalmicrobiotainpostmenopausaloestrogenreceptorpositivebreastcancerpatientstreatedwithneoadjuvantchemotherapy AT marjoleinlsmidt changesinintestinalmicrobiotainpostmenopausaloestrogenreceptorpositivebreastcancerpatientstreatedwithneoadjuvantchemotherapy |