Multi Epitope-Based Vaccine Design for Protection Against Mycobacterium tuberculosis and SARS-CoV-2 Coinfection
Dian Ayu Eka Pitaloka,1,2 Afifah Izzati,3 Siti Rafa Amirah,3 Luqman Abdan Syakuran4 1Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, 45363, Indonesia; 2Center of Excellence in Higher Education for Pharmaceutical Care Innovation, Universitas P...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2022-08-01
|
| Series: | Advances and Applications in Bioinformatics and Chemistry |
| Subjects: | |
| Online Access: | https://www.dovepress.com/multi-epitope-based-vaccine-design-for-protection-against-mycobacteriu-peer-reviewed-fulltext-article-AABC |
| _version_ | 1811345246315872256 |
|---|---|
| author | Pitaloka DAE Izzati A Amirah SR Syakuran LA |
| author_facet | Pitaloka DAE Izzati A Amirah SR Syakuran LA |
| author_sort | Pitaloka DAE |
| collection | DOAJ |
| description | Dian Ayu Eka Pitaloka,1,2 Afifah Izzati,3 Siti Rafa Amirah,3 Luqman Abdan Syakuran4 1Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, 45363, Indonesia; 2Center of Excellence in Higher Education for Pharmaceutical Care Innovation, Universitas Padjadjaran, Sumedang, 45363, Indonesia; 3Pharmacy Program, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, 45363, Indonesia; 4Faculty of Biology, Jenderal Soedirman University, Grendeng Purwokerto, 53122, IndonesiaCorrespondence: Dian Ayu Eka Pitaloka, Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, 45363, Indonesia, Tel +62-22-84288812, Email dian.pitaloka@unpad.ac.idBackground: A prophylactic and immunotherapeutic vaccine for Mycobacterium tuberculosis (MTB) and SARS-CoV-2 coinfection needs to be developed for a proactive and effective therapeutic approach. Therefore, this study aims to use immunoinformatics to design a multi-epitope vaccine for protection against MTB and SARS-CoV-2 coinfection.Methods: The bioinformatic techniques were used to screen and construct potential epitopes from outer membrane protein A Rv0899 of MTB and spike glycoprotein of SARS-CoV-2 for B and T cells. The antigenicity, allergenicity, and several physiochemical properties of the developed multi-epitope vaccination were then evaluated. Additionally, molecular docking and normal mode analysis (NMA) were utilized in evaluating the vaccine’s immunogenicity and complex stability.Results: Selected proteins and predicted epitopes suggest that the vaccine prediction can be helpful in the protection against both SARS-CoV-2 and MTB coinfection. Through docking molecular and NMA, the vaccine-TLR4 protein interaction was predicted to be efficient with a high level of IgG, T-helper cells, T-cytotoxic cells, andIFN-γ.Conclusion: This epitope-based vaccine is a potentially attractive tool for SARS-CoV-2 and MTB coinfection vaccine development.Graphical Abstract: Keywords: SARS-CoV-2, Mycobacterium tuberculosis, multi-epitope vaccine, docking simulation |
| first_indexed | 2024-04-13T20:00:14Z |
| format | Article |
| id | doaj.art-428e31f1826c4e36a5157d29c27bcc71 |
| institution | Directory Open Access Journal |
| issn | 1178-6949 |
| language | English |
| last_indexed | 2024-04-13T20:00:14Z |
| publishDate | 2022-08-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Advances and Applications in Bioinformatics and Chemistry |
| spelling | doaj.art-428e31f1826c4e36a5157d29c27bcc712022-12-22T02:32:14ZengDove Medical PressAdvances and Applications in Bioinformatics and Chemistry1178-69492022-08-01Volume 15435777011Multi Epitope-Based Vaccine Design for Protection Against Mycobacterium tuberculosis and SARS-CoV-2 CoinfectionPitaloka DAEIzzati AAmirah SRSyakuran LADian Ayu Eka Pitaloka,1,2 Afifah Izzati,3 Siti Rafa Amirah,3 Luqman Abdan Syakuran4 1Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, 45363, Indonesia; 2Center of Excellence in Higher Education for Pharmaceutical Care Innovation, Universitas Padjadjaran, Sumedang, 45363, Indonesia; 3Pharmacy Program, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, 45363, Indonesia; 4Faculty of Biology, Jenderal Soedirman University, Grendeng Purwokerto, 53122, IndonesiaCorrespondence: Dian Ayu Eka Pitaloka, Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, 45363, Indonesia, Tel +62-22-84288812, Email dian.pitaloka@unpad.ac.idBackground: A prophylactic and immunotherapeutic vaccine for Mycobacterium tuberculosis (MTB) and SARS-CoV-2 coinfection needs to be developed for a proactive and effective therapeutic approach. Therefore, this study aims to use immunoinformatics to design a multi-epitope vaccine for protection against MTB and SARS-CoV-2 coinfection.Methods: The bioinformatic techniques were used to screen and construct potential epitopes from outer membrane protein A Rv0899 of MTB and spike glycoprotein of SARS-CoV-2 for B and T cells. The antigenicity, allergenicity, and several physiochemical properties of the developed multi-epitope vaccination were then evaluated. Additionally, molecular docking and normal mode analysis (NMA) were utilized in evaluating the vaccine’s immunogenicity and complex stability.Results: Selected proteins and predicted epitopes suggest that the vaccine prediction can be helpful in the protection against both SARS-CoV-2 and MTB coinfection. Through docking molecular and NMA, the vaccine-TLR4 protein interaction was predicted to be efficient with a high level of IgG, T-helper cells, T-cytotoxic cells, andIFN-γ.Conclusion: This epitope-based vaccine is a potentially attractive tool for SARS-CoV-2 and MTB coinfection vaccine development.Graphical Abstract: Keywords: SARS-CoV-2, Mycobacterium tuberculosis, multi-epitope vaccine, docking simulationhttps://www.dovepress.com/multi-epitope-based-vaccine-design-for-protection-against-mycobacteriu-peer-reviewed-fulltext-article-AABCsars-cov-2mycobacterium tuberculosismulti-epitope vaccinedocking simulation |
| spellingShingle | Pitaloka DAE Izzati A Amirah SR Syakuran LA Multi Epitope-Based Vaccine Design for Protection Against Mycobacterium tuberculosis and SARS-CoV-2 Coinfection Advances and Applications in Bioinformatics and Chemistry sars-cov-2 mycobacterium tuberculosis multi-epitope vaccine docking simulation |
| title | Multi Epitope-Based Vaccine Design for Protection Against Mycobacterium tuberculosis and SARS-CoV-2 Coinfection |
| title_full | Multi Epitope-Based Vaccine Design for Protection Against Mycobacterium tuberculosis and SARS-CoV-2 Coinfection |
| title_fullStr | Multi Epitope-Based Vaccine Design for Protection Against Mycobacterium tuberculosis and SARS-CoV-2 Coinfection |
| title_full_unstemmed | Multi Epitope-Based Vaccine Design for Protection Against Mycobacterium tuberculosis and SARS-CoV-2 Coinfection |
| title_short | Multi Epitope-Based Vaccine Design for Protection Against Mycobacterium tuberculosis and SARS-CoV-2 Coinfection |
| title_sort | multi epitope based vaccine design for protection against mycobacterium tuberculosis and sars cov 2 coinfection |
| topic | sars-cov-2 mycobacterium tuberculosis multi-epitope vaccine docking simulation |
| url | https://www.dovepress.com/multi-epitope-based-vaccine-design-for-protection-against-mycobacteriu-peer-reviewed-fulltext-article-AABC |
| work_keys_str_mv | AT pitalokadae multiepitopebasedvaccinedesignforprotectionagainstmycobacteriumtuberculosisandsarscov2coinfection AT izzatia multiepitopebasedvaccinedesignforprotectionagainstmycobacteriumtuberculosisandsarscov2coinfection AT amirahsr multiepitopebasedvaccinedesignforprotectionagainstmycobacteriumtuberculosisandsarscov2coinfection AT syakuranla multiepitopebasedvaccinedesignforprotectionagainstmycobacteriumtuberculosisandsarscov2coinfection |