Expression and significance of pigment epithelium-derived factor and vascular endothelial growth factor in lens epithelial cells of diabetic patients with cataract
AIM: To observe the expression levels of pigment epithelium-derived factor(PEDF)and vascular endothelial growth factor(VEGF)in lens epithelial cells(LECs)of diabetic patients with age-related cataract, and explore the pathogenesis of diabetic with age-related cataract.METHODS: Retrospectively study....
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2023-01-01
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| Series: | Guoji Yanke Zazhi |
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| Online Access: | http://ies.ijo.cn/cn_publish/2023/1/202301021.pdf |
| _version_ | 1797962195875659776 |
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| author | Ya-Ru Hu Yue Chen Xue Han Ya-Ru Chen Zhu-Yu Ma Jian-Feng Wang |
| author_facet | Ya-Ru Hu Yue Chen Xue Han Ya-Ru Chen Zhu-Yu Ma Jian-Feng Wang |
| author_sort | Ya-Ru Hu |
| collection | DOAJ |
| description | AIM: To observe the expression levels of pigment epithelium-derived factor(PEDF)and vascular endothelial growth factor(VEGF)in lens epithelial cells(LECs)of diabetic patients with age-related cataract, and explore the pathogenesis of diabetic with age-related cataract.METHODS: Retrospectively study. A total of 30 patients with age-related cataract(ARC group)and 30 patients with type 2 diabetes mellitus combined with age-related cataract(DC group)who treated at the Department of Ophthalmology of the First Affiliated Hospital of Bengbu Medical College from August 2020 to April 2021 were collected. Anterior capsule specimens with a diameter of 5.5-6.0mm in the central region of the intraoperative eye were collected during phacoemulsification. The protein expression levels of PEDF and VEGF in LECs were detected by Western-blot. Quantitative real-time PCR(qRT-PCR)was used to detect the relative mRNA expression levels of PEDF and VEGF.RESULTS:PEDF and VEGF were expressed in LECs of both groups, and the relative expression of VEGF mRNA in DC group was 1.364±0.062, which was higher than that of ARC group(1.000±0.0; P<0.01). The relative mRNA expression level of PEDF was 0.398±0.053, which was significantly lower than 1.000±0.0 in the ARC group(P<0.001). The expression of VEGF protein in LECs was 2.053±0.026 in DC group and 1.680±0.064 in ARC group, respectively. Meanwhile, the expression of PEDF protein was 0.579±0.045 in DC group and 1.058±0.007 in ARC group(all P<0.01).CONCLUSION:The changes of PEDF and VEGF expression levels in LECs may be related to the occurrence and development of cataract in diabetic patients. |
| first_indexed | 2024-04-11T01:09:35Z |
| format | Article |
| id | doaj.art-42901135178640b982ef6515058545bd |
| institution | Directory Open Access Journal |
| issn | 1672-5123 |
| language | English |
| last_indexed | 2024-04-11T01:09:35Z |
| publishDate | 2023-01-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | Guoji Yanke Zazhi |
| spelling | doaj.art-42901135178640b982ef6515058545bd2023-01-04T07:41:52ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51232023-01-0123110911210.3980/j.issn.1672-5123.2023.1.21202301021Expression and significance of pigment epithelium-derived factor and vascular endothelial growth factor in lens epithelial cells of diabetic patients with cataractYa-Ru Hu0Yue Chen1Xue Han2Ya-Ru Chen3Zhu-Yu Ma4Jian-Feng Wang5Department of Ophthalmology, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, Anhui Province, China; Bengbu Medical College, Bengbu 233030, Anhui Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, Anhui Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, Anhui Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, Anhui Province, ChinaBengbu Medical College, Bengbu 233030, Anhui Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, Anhui Province, ChinaAIM: To observe the expression levels of pigment epithelium-derived factor(PEDF)and vascular endothelial growth factor(VEGF)in lens epithelial cells(LECs)of diabetic patients with age-related cataract, and explore the pathogenesis of diabetic with age-related cataract.METHODS: Retrospectively study. A total of 30 patients with age-related cataract(ARC group)and 30 patients with type 2 diabetes mellitus combined with age-related cataract(DC group)who treated at the Department of Ophthalmology of the First Affiliated Hospital of Bengbu Medical College from August 2020 to April 2021 were collected. Anterior capsule specimens with a diameter of 5.5-6.0mm in the central region of the intraoperative eye were collected during phacoemulsification. The protein expression levels of PEDF and VEGF in LECs were detected by Western-blot. Quantitative real-time PCR(qRT-PCR)was used to detect the relative mRNA expression levels of PEDF and VEGF.RESULTS:PEDF and VEGF were expressed in LECs of both groups, and the relative expression of VEGF mRNA in DC group was 1.364±0.062, which was higher than that of ARC group(1.000±0.0; P<0.01). The relative mRNA expression level of PEDF was 0.398±0.053, which was significantly lower than 1.000±0.0 in the ARC group(P<0.001). The expression of VEGF protein in LECs was 2.053±0.026 in DC group and 1.680±0.064 in ARC group, respectively. Meanwhile, the expression of PEDF protein was 0.579±0.045 in DC group and 1.058±0.007 in ARC group(all P<0.01).CONCLUSION:The changes of PEDF and VEGF expression levels in LECs may be related to the occurrence and development of cataract in diabetic patients.http://ies.ijo.cn/cn_publish/2023/1/202301021.pdfdiabetes mellitus with cataractlens epithelial cells(lecs)pigment epithelium-derived factor(pedf)vascular endothelial growth factor(vegf) |
| spellingShingle | Ya-Ru Hu Yue Chen Xue Han Ya-Ru Chen Zhu-Yu Ma Jian-Feng Wang Expression and significance of pigment epithelium-derived factor and vascular endothelial growth factor in lens epithelial cells of diabetic patients with cataract Guoji Yanke Zazhi diabetes mellitus with cataract lens epithelial cells(lecs) pigment epithelium-derived factor(pedf) vascular endothelial growth factor(vegf) |
| title | Expression and significance of pigment epithelium-derived factor and vascular endothelial growth factor in lens epithelial cells of diabetic patients with cataract |
| title_full | Expression and significance of pigment epithelium-derived factor and vascular endothelial growth factor in lens epithelial cells of diabetic patients with cataract |
| title_fullStr | Expression and significance of pigment epithelium-derived factor and vascular endothelial growth factor in lens epithelial cells of diabetic patients with cataract |
| title_full_unstemmed | Expression and significance of pigment epithelium-derived factor and vascular endothelial growth factor in lens epithelial cells of diabetic patients with cataract |
| title_short | Expression and significance of pigment epithelium-derived factor and vascular endothelial growth factor in lens epithelial cells of diabetic patients with cataract |
| title_sort | expression and significance of pigment epithelium derived factor and vascular endothelial growth factor in lens epithelial cells of diabetic patients with cataract |
| topic | diabetes mellitus with cataract lens epithelial cells(lecs) pigment epithelium-derived factor(pedf) vascular endothelial growth factor(vegf) |
| url | http://ies.ijo.cn/cn_publish/2023/1/202301021.pdf |
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