Icariin-conditioned serum combined with chitosan attenuates cartilage injury in rabbit knees with osteochondral defect
Abstract Background Knee osteoarthritis (KOA) is one of the most common degenerative diseases. Its development is closely related to cartilage injury and subchondral bone remodeling homeostasis. In the present study, we combined icariin-conditioned serum (ICS) with thiolated chitosan (CSSH), a mater...
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | Journal of Orthopaedic Surgery and Research |
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| Online Access: | https://doi.org/10.1186/s13018-023-03607-w |
| _version_ | 1797864008203632640 |
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| author | Juntao Zhang Fangyang Fan Chao Zhang Aifeng Liu Man Shang Lin Meng |
| author_facet | Juntao Zhang Fangyang Fan Chao Zhang Aifeng Liu Man Shang Lin Meng |
| author_sort | Juntao Zhang |
| collection | DOAJ |
| description | Abstract Background Knee osteoarthritis (KOA) is one of the most common degenerative diseases. Its development is closely related to cartilage injury and subchondral bone remodeling homeostasis. In the present study, we combined icariin-conditioned serum (ICS) with thiolated chitosan (CSSH), a material widely used in tissue engineering for cartilage repair, to demonstrate its effect on the repair of cartilage damage and abnormal subchondral remodeling. Methods New Zealand rabbits were undergoing surgery for cartilage defect, and joint cavity injection was performed in each group with 0.5 mL normal saline (NS), ICS, CSSH and ICS-CSSH in the right joint every week for five times. Positioning performance was observed using VICON motion capture system. Glycosaminoglycans (GAG) secretion of articular fluid was assessed. Osteoarthritis Research Society International (OARSI) score and immunohistochemical (IHC) analysis including H&E, Safranin O and collagen II staining were employed to evaluate the morphologic repair of cartilage and subchondral bone. mRNA expression of COL2A1, MMP13 and ADAMTS5 was detected in chondrocytes from injury area. Results ICS combined with CSSH attenuated cartilage injury and abnormal subchondral remodeling in rabbits with KOA. ICS and CSSH groups showed slight improvement in positioning performance, while ICS-CSSH group exhibited better positioning performance. ICS-CSSH group showed increased GAG secretion of articular fluid and expression of COL2A1 in articular chondrocytes. Furthermore, both macroscopic observation and IHC analysis showed femoral condyle in ICS-CSSH rabbits were repaired with more native cartilage and subchondral bone regeneration. Conclusions ICS combined with CSSH could promote the repair of osteochondral defect and stabilize subchondral bone remodeling in rabbit knees. |
| first_indexed | 2024-04-09T22:45:44Z |
| format | Article |
| id | doaj.art-42995c7e4d2e4b318637c41e89db84bf |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-09T22:45:44Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-42995c7e4d2e4b318637c41e89db84bf2023-03-22T11:52:35ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2023-02-0118111010.1186/s13018-023-03607-wIcariin-conditioned serum combined with chitosan attenuates cartilage injury in rabbit knees with osteochondral defectJuntao Zhang0Fangyang Fan1Chao Zhang2Aifeng Liu3Man Shang4Lin Meng5Academy of Medical Engineering and Traditional Medicine, Tianjin UniversityOrthopedics Department, National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, The First Teaching Hospital of Tianjin University of Traditional Chinese MedicineOrthopedics Department, National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, The First Teaching Hospital of Tianjin University of Traditional Chinese MedicineOrthopedics Department, National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, The First Teaching Hospital of Tianjin University of Traditional Chinese MedicineSchool of Basic Medical Sciences, Tianjin Medical UniversityAcademy of Medical Engineering and Traditional Medicine, Tianjin UniversityAbstract Background Knee osteoarthritis (KOA) is one of the most common degenerative diseases. Its development is closely related to cartilage injury and subchondral bone remodeling homeostasis. In the present study, we combined icariin-conditioned serum (ICS) with thiolated chitosan (CSSH), a material widely used in tissue engineering for cartilage repair, to demonstrate its effect on the repair of cartilage damage and abnormal subchondral remodeling. Methods New Zealand rabbits were undergoing surgery for cartilage defect, and joint cavity injection was performed in each group with 0.5 mL normal saline (NS), ICS, CSSH and ICS-CSSH in the right joint every week for five times. Positioning performance was observed using VICON motion capture system. Glycosaminoglycans (GAG) secretion of articular fluid was assessed. Osteoarthritis Research Society International (OARSI) score and immunohistochemical (IHC) analysis including H&E, Safranin O and collagen II staining were employed to evaluate the morphologic repair of cartilage and subchondral bone. mRNA expression of COL2A1, MMP13 and ADAMTS5 was detected in chondrocytes from injury area. Results ICS combined with CSSH attenuated cartilage injury and abnormal subchondral remodeling in rabbits with KOA. ICS and CSSH groups showed slight improvement in positioning performance, while ICS-CSSH group exhibited better positioning performance. ICS-CSSH group showed increased GAG secretion of articular fluid and expression of COL2A1 in articular chondrocytes. Furthermore, both macroscopic observation and IHC analysis showed femoral condyle in ICS-CSSH rabbits were repaired with more native cartilage and subchondral bone regeneration. Conclusions ICS combined with CSSH could promote the repair of osteochondral defect and stabilize subchondral bone remodeling in rabbit knees.https://doi.org/10.1186/s13018-023-03607-wOsteoarthritisIcariinChitosanCartilage repairSubchondral bone |
| spellingShingle | Juntao Zhang Fangyang Fan Chao Zhang Aifeng Liu Man Shang Lin Meng Icariin-conditioned serum combined with chitosan attenuates cartilage injury in rabbit knees with osteochondral defect Journal of Orthopaedic Surgery and Research Osteoarthritis Icariin Chitosan Cartilage repair Subchondral bone |
| title | Icariin-conditioned serum combined with chitosan attenuates cartilage injury in rabbit knees with osteochondral defect |
| title_full | Icariin-conditioned serum combined with chitosan attenuates cartilage injury in rabbit knees with osteochondral defect |
| title_fullStr | Icariin-conditioned serum combined with chitosan attenuates cartilage injury in rabbit knees with osteochondral defect |
| title_full_unstemmed | Icariin-conditioned serum combined with chitosan attenuates cartilage injury in rabbit knees with osteochondral defect |
| title_short | Icariin-conditioned serum combined with chitosan attenuates cartilage injury in rabbit knees with osteochondral defect |
| title_sort | icariin conditioned serum combined with chitosan attenuates cartilage injury in rabbit knees with osteochondral defect |
| topic | Osteoarthritis Icariin Chitosan Cartilage repair Subchondral bone |
| url | https://doi.org/10.1186/s13018-023-03607-w |
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