Bioassays for Identifying and Characterizing Plant Regulatory Peptides
Plant peptides are a new frontier in plant biology, owing to their key regulatory roles in plant growth, development, and stress responses. Synthetic peptides are promising biological agents that can be used to improve crop growth and protection in an environmentally sustainable manner. Plant regula...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/12/1795 |
| _version_ | 1797381795190145024 |
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| author | Alexander Skripnikov |
| author_facet | Alexander Skripnikov |
| author_sort | Alexander Skripnikov |
| collection | DOAJ |
| description | Plant peptides are a new frontier in plant biology, owing to their key regulatory roles in plant growth, development, and stress responses. Synthetic peptides are promising biological agents that can be used to improve crop growth and protection in an environmentally sustainable manner. Plant regulatory peptides identified in pioneering research, including systemin, PSK, HypSys, RALPH, <i>At</i>Pep1, CLV3, TDIF, CLE, and RGF/GLV/CLEL, hold promise for crop improvement as potent regulators of plant growth and defense. Mass spectrometry and bioinformatics are greatly facilitating the discovery and identification of new plant peptides. The biological functions of most novel plant peptides remain to be elucidated. Bioassays are an essential part in studying the biological activity of identified and putative plant peptides. Root growth assays and cultivated plant cell cultures are widely used to evaluate the regulatory potential of plant peptides during growth, differentiation, and stress reactions. These bioassays can be used as universal approaches for screening peptides from different plant species. Development of high-throughput bioassays can facilitate the screening of large numbers of identified and putative plant peptides, which have recently been discovered but remain uncharacterized for biological activity. |
| first_indexed | 2024-03-08T20:57:34Z |
| format | Article |
| id | doaj.art-429e346d8b5c475d994e55e6747e3960 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-08T20:57:34Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-429e346d8b5c475d994e55e6747e39602023-12-22T13:56:09ZengMDPI AGBiomolecules2218-273X2023-12-011312179510.3390/biom13121795Bioassays for Identifying and Characterizing Plant Regulatory PeptidesAlexander Skripnikov0Shemyakin—Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya St. 16/10, 119997 Moscow, RussiaPlant peptides are a new frontier in plant biology, owing to their key regulatory roles in plant growth, development, and stress responses. Synthetic peptides are promising biological agents that can be used to improve crop growth and protection in an environmentally sustainable manner. Plant regulatory peptides identified in pioneering research, including systemin, PSK, HypSys, RALPH, <i>At</i>Pep1, CLV3, TDIF, CLE, and RGF/GLV/CLEL, hold promise for crop improvement as potent regulators of plant growth and defense. Mass spectrometry and bioinformatics are greatly facilitating the discovery and identification of new plant peptides. The biological functions of most novel plant peptides remain to be elucidated. Bioassays are an essential part in studying the biological activity of identified and putative plant peptides. Root growth assays and cultivated plant cell cultures are widely used to evaluate the regulatory potential of plant peptides during growth, differentiation, and stress reactions. These bioassays can be used as universal approaches for screening peptides from different plant species. Development of high-throughput bioassays can facilitate the screening of large numbers of identified and putative plant peptides, which have recently been discovered but remain uncharacterized for biological activity.https://www.mdpi.com/2218-273X/13/12/1795plant peptidesbioassaymass spectrometryputative peptidesregulatory peptidesroot growth assay |
| spellingShingle | Alexander Skripnikov Bioassays for Identifying and Characterizing Plant Regulatory Peptides Biomolecules plant peptides bioassay mass spectrometry putative peptides regulatory peptides root growth assay |
| title | Bioassays for Identifying and Characterizing Plant Regulatory Peptides |
| title_full | Bioassays for Identifying and Characterizing Plant Regulatory Peptides |
| title_fullStr | Bioassays for Identifying and Characterizing Plant Regulatory Peptides |
| title_full_unstemmed | Bioassays for Identifying and Characterizing Plant Regulatory Peptides |
| title_short | Bioassays for Identifying and Characterizing Plant Regulatory Peptides |
| title_sort | bioassays for identifying and characterizing plant regulatory peptides |
| topic | plant peptides bioassay mass spectrometry putative peptides regulatory peptides root growth assay |
| url | https://www.mdpi.com/2218-273X/13/12/1795 |
| work_keys_str_mv | AT alexanderskripnikov bioassaysforidentifyingandcharacterizingplantregulatorypeptides |