Results of in vitro chemotherapy of apple cv. Fragrance - Short communication
The effect of the chemotherapy with ribavirin on the elimination of the pome fruit viruses from in vitro grown plants of infected apple cv. Fragnance has been investigated. The results of ELISA and RT-PCR testing proved the presence of mixed infection of Apple stem pitting virus (ASPV), Apple chloro...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2013-12-01
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| Series: | Horticultural Science |
| Subjects: | |
| Online Access: | https://hortsci.agriculturejournals.cz/artkey/hor-201304-0008_results-of-in-vitro-chemotherapy-of-apple-cv-fragrance-short-communication.php |
| _version_ | 1828008282688585728 |
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| author | F. Paprštein J. Sedlák L. Svobodová J. Polák S. Gadiou |
| author_facet | F. Paprštein J. Sedlák L. Svobodová J. Polák S. Gadiou |
| author_sort | F. Paprštein |
| collection | DOAJ |
| description | The effect of the chemotherapy with ribavirin on the elimination of the pome fruit viruses from in vitro grown plants of infected apple cv. Fragnance has been investigated. The results of ELISA and RT-PCR testing proved the presence of mixed infection of Apple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV) and Apple stem grooving virus (ASGV) in the initial field-grown tree of this apple cultivar. Obtained actively growing in vitro shoots with well-developed leaves and shoot tips were subsequently used for chemotherapy with ribavirin. Attempts to fully eliminate viruses by ribavirin in lower concentration 20 mg/l were not successful. However in vitro plants of one mericlone (FR1R20) sanitated from ASPV and ASGV, which were infected with ACLSV only after the first chemotherapy cycle, were subjected to repeated treatment on medium with higher ribavirin concentration 100 mg/l. The success of chemotherapy with ribavirin at 100 mg/l was 76% for ACLSV elimination after the second round. In the course of both chemotherapy cycles (20 mg/l and 100 mg/l), in vitro plants did not display symptoms of phytotoxicity. |
| first_indexed | 2024-04-10T08:22:34Z |
| format | Article |
| id | doaj.art-429e3bddbd9c4ffc92b66de7732eaf06 |
| institution | Directory Open Access Journal |
| issn | 0862-867X 1805-9333 |
| language | English |
| last_indexed | 2024-04-10T08:22:34Z |
| publishDate | 2013-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Horticultural Science |
| spelling | doaj.art-429e3bddbd9c4ffc92b66de7732eaf062023-02-23T03:34:49ZengCzech Academy of Agricultural SciencesHorticultural Science0862-867X1805-93332013-12-0140418619010.17221/37/2013-HORTSCIhor-201304-0008Results of in vitro chemotherapy of apple cv. Fragrance - Short communicationF. Paprštein0J. Sedlák1L. Svobodová2J. Polák3S. Gadiou4Research and Breeding Institute of Pomology Holovousy Ltd., Holovousy, Czech RepublicResearch and Breeding Institute of Pomology Holovousy Ltd., Holovousy, Czech RepublicResearch and Breeding Institute of Pomology Holovousy Ltd., Holovousy, Czech RepublicCrop Research Institute, Prague, Czech RepublicCrop Research Institute, Prague, Czech RepublicThe effect of the chemotherapy with ribavirin on the elimination of the pome fruit viruses from in vitro grown plants of infected apple cv. Fragnance has been investigated. The results of ELISA and RT-PCR testing proved the presence of mixed infection of Apple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV) and Apple stem grooving virus (ASGV) in the initial field-grown tree of this apple cultivar. Obtained actively growing in vitro shoots with well-developed leaves and shoot tips were subsequently used for chemotherapy with ribavirin. Attempts to fully eliminate viruses by ribavirin in lower concentration 20 mg/l were not successful. However in vitro plants of one mericlone (FR1R20) sanitated from ASPV and ASGV, which were infected with ACLSV only after the first chemotherapy cycle, were subjected to repeated treatment on medium with higher ribavirin concentration 100 mg/l. The success of chemotherapy with ribavirin at 100 mg/l was 76% for ACLSV elimination after the second round. In the course of both chemotherapy cycles (20 mg/l and 100 mg/l), in vitro plants did not display symptoms of phytotoxicity.https://hortsci.agriculturejournals.cz/artkey/hor-201304-0008_results-of-in-vitro-chemotherapy-of-apple-cv-fragrance-short-communication.phpribavirinpcrelisavirus detectionvirus eliminationvirus infection |
| spellingShingle | F. Paprštein J. Sedlák L. Svobodová J. Polák S. Gadiou Results of in vitro chemotherapy of apple cv. Fragrance - Short communication Horticultural Science ribavirin pcr elisa virus detection virus elimination virus infection |
| title | Results of in vitro chemotherapy of apple cv. Fragrance - Short communication |
| title_full | Results of in vitro chemotherapy of apple cv. Fragrance - Short communication |
| title_fullStr | Results of in vitro chemotherapy of apple cv. Fragrance - Short communication |
| title_full_unstemmed | Results of in vitro chemotherapy of apple cv. Fragrance - Short communication |
| title_short | Results of in vitro chemotherapy of apple cv. Fragrance - Short communication |
| title_sort | results of in vitro chemotherapy of apple cv fragrance short communication |
| topic | ribavirin pcr elisa virus detection virus elimination virus infection |
| url | https://hortsci.agriculturejournals.cz/artkey/hor-201304-0008_results-of-in-vitro-chemotherapy-of-apple-cv-fragrance-short-communication.php |
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