Cry1Ac Mixed with Gentamicin Influences the Intestinal Microbial Diversity and Community Composition of Pink Bollworms
Pink bollworms severely affect the production of cotton. The method currently used for pink bollworm control is the planting of Bt (<i>Bacillus thuringiensis</i>) protein-expressing transgenic cotton. However, pink bollworms can develop strong resistance to Bt proteins in transgenic cott...
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MDPI AG
2023-12-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/14/1/58 |
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| author | Zhan-Bin Sun Ya-Feng Hu Han-Jian Song Sheng-Bo Cong Ling Wang |
| author_facet | Zhan-Bin Sun Ya-Feng Hu Han-Jian Song Sheng-Bo Cong Ling Wang |
| author_sort | Zhan-Bin Sun |
| collection | DOAJ |
| description | Pink bollworms severely affect the production of cotton. The method currently used for pink bollworm control is the planting of Bt (<i>Bacillus thuringiensis</i>) protein-expressing transgenic cotton. However, pink bollworms can develop strong resistance to Bt proteins in transgenic cotton because of the large planting area and long planting time of this crop, which severely affects the control of pink bollworms. Intestinal microorganisms play very important roles in insect growth, development and Bt resistance. However, the effect of intestinal microorganisms on pink bollworm Bt resistance is still unclear. The current study aimed to analyze the effect of intestinal microorganisms on the Bt resistance of pink bollworms. Intestinal microorganisms associated with Bt resistance were initially screened through Illumina MiSeq sequencing and analysis. The results showed that feeding with a mixture of gentamicin, Cry1Ac and an artificial diet could significantly increase the mortality of pink bollworm larvae compared with feeding with of a mixture of Cry1Ac and an artificial diet or an artificial diet alone. The microbial diversity, community structure and composition of the pink bollworm larval intestine were significantly influenced by feeding with a mixture of gentamicin, Cry1Ac and an artificial diet. Several intestinal bacteria with significantly altered abundances after treatment with gentamicin were preliminarily screened as potential resources for addressing Bt toxicity. This study provides useful strategies for addressing the Bt resistance of pink bollworms. |
| first_indexed | 2024-03-08T10:44:11Z |
| format | Article |
| id | doaj.art-42a533911c1a4dd388b0e49b08234b6d |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-08T10:44:11Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-42a533911c1a4dd388b0e49b08234b6d2024-01-26T17:20:38ZengMDPI AGLife2075-17292023-12-011415810.3390/life14010058Cry1Ac Mixed with Gentamicin Influences the Intestinal Microbial Diversity and Community Composition of Pink BollwormsZhan-Bin Sun0Ya-Feng Hu1Han-Jian Song2Sheng-Bo Cong3Ling Wang4China Food Flavor and Nutrition Health Innovation Center, Beijing Technology and Business University, Beijing 100048, ChinaChina Food Flavor and Nutrition Health Innovation Center, Beijing Technology and Business University, Beijing 100048, ChinaChina Food Flavor and Nutrition Health Innovation Center, Beijing Technology and Business University, Beijing 100048, ChinaKey Laboratory of Integrated Pest Management on Crops in Central China, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Crop Disease, Insect Pests and Weeds Control, Institute of Plant Protection and Soil Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaKey Laboratory of Integrated Pest Management on Crops in Central China, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Crop Disease, Insect Pests and Weeds Control, Institute of Plant Protection and Soil Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaPink bollworms severely affect the production of cotton. The method currently used for pink bollworm control is the planting of Bt (<i>Bacillus thuringiensis</i>) protein-expressing transgenic cotton. However, pink bollworms can develop strong resistance to Bt proteins in transgenic cotton because of the large planting area and long planting time of this crop, which severely affects the control of pink bollworms. Intestinal microorganisms play very important roles in insect growth, development and Bt resistance. However, the effect of intestinal microorganisms on pink bollworm Bt resistance is still unclear. The current study aimed to analyze the effect of intestinal microorganisms on the Bt resistance of pink bollworms. Intestinal microorganisms associated with Bt resistance were initially screened through Illumina MiSeq sequencing and analysis. The results showed that feeding with a mixture of gentamicin, Cry1Ac and an artificial diet could significantly increase the mortality of pink bollworm larvae compared with feeding with of a mixture of Cry1Ac and an artificial diet or an artificial diet alone. The microbial diversity, community structure and composition of the pink bollworm larval intestine were significantly influenced by feeding with a mixture of gentamicin, Cry1Ac and an artificial diet. Several intestinal bacteria with significantly altered abundances after treatment with gentamicin were preliminarily screened as potential resources for addressing Bt toxicity. This study provides useful strategies for addressing the Bt resistance of pink bollworms.https://www.mdpi.com/2075-1729/14/1/58alpha diversityPCoA analysisLEfSe analysisBt resistanceintestinal microorganismsIllumina MiSeq sequencing and analysis |
| spellingShingle | Zhan-Bin Sun Ya-Feng Hu Han-Jian Song Sheng-Bo Cong Ling Wang Cry1Ac Mixed with Gentamicin Influences the Intestinal Microbial Diversity and Community Composition of Pink Bollworms Life alpha diversity PCoA analysis LEfSe analysis Bt resistance intestinal microorganisms Illumina MiSeq sequencing and analysis |
| title | Cry1Ac Mixed with Gentamicin Influences the Intestinal Microbial Diversity and Community Composition of Pink Bollworms |
| title_full | Cry1Ac Mixed with Gentamicin Influences the Intestinal Microbial Diversity and Community Composition of Pink Bollworms |
| title_fullStr | Cry1Ac Mixed with Gentamicin Influences the Intestinal Microbial Diversity and Community Composition of Pink Bollworms |
| title_full_unstemmed | Cry1Ac Mixed with Gentamicin Influences the Intestinal Microbial Diversity and Community Composition of Pink Bollworms |
| title_short | Cry1Ac Mixed with Gentamicin Influences the Intestinal Microbial Diversity and Community Composition of Pink Bollworms |
| title_sort | cry1ac mixed with gentamicin influences the intestinal microbial diversity and community composition of pink bollworms |
| topic | alpha diversity PCoA analysis LEfSe analysis Bt resistance intestinal microorganisms Illumina MiSeq sequencing and analysis |
| url | https://www.mdpi.com/2075-1729/14/1/58 |
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