Simultaneous determination of minoxidil and minoxidil sulfate by high-performance liquid chromatography with UV-detection and its applications
Aims: Minoxidil is a hair growth drug for treating androgenetic alopecia. Although minoxidil is generally administered as a topical formulation, this prodrug must be converted to its active form (minoxidil sulfate) by sulfotransferase in hair follicle tissue. Therefore, its effect may be affected by...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Medicine in Drug Discovery |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590098620300373 |
| _version_ | 1818257187378561024 |
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| author | Mio Ichida Chiharu Fujita Rena Sumie Ryotaro Miyano Hajime Inoue |
| author_facet | Mio Ichida Chiharu Fujita Rena Sumie Ryotaro Miyano Hajime Inoue |
| author_sort | Mio Ichida |
| collection | DOAJ |
| description | Aims: Minoxidil is a hair growth drug for treating androgenetic alopecia. Although minoxidil is generally administered as a topical formulation, this prodrug must be converted to its active form (minoxidil sulfate) by sulfotransferase in hair follicle tissue. Therefore, its effect may be affected by the sulfureting of minoxidil and de-sulfureting of minoxidil sulfate in hair follicles. To investigate the biotransformation of minoxidil sulfate to minoxidil in hair follicle component cells, a method for simultaneous determination of minoxidil and minoxidil sulfate by high-performance liquid chromatography with UV-detection was established. Main methods: Minoxidil and minoxidil sulfate were separated by a reversed-phase chromatography. Minoxidil sulfate was incubated with human hair follicle keratinocyte- and dermal papilla cell-derived arylsulfatases as well as snail-derived sulfatase. The enzyme mixture was applied on HPLC system directly. Key findings: Minoxidil and minoxidil sulfate were separated simultaneously. The limit of detection of minoxidil and minoxidil sulfate was 25 and 100 pg inj−1, respectively. Snail-derived sulfatase as well as human hair follicle keratinocyte- and dermal papilla cell-derived arylsulfatases hydrolyzed minoxidil sulfate to minoxidil. Arylsulfatase in hair follicle metabolized the active form of minoxidil to the inactive form. Inactivation of active minoxidil (sulfate) was inhibited by the natural substrate of arylsulfatase A, ascorbate-2-sulfate. Significance: Arylsulfatase inhibitors may sustain the effect of minoxidil sulfate in AGA therapy. The developed technique was effective for studies of minoxidil metabolism and bioavailability. |
| first_indexed | 2024-12-12T17:39:40Z |
| format | Article |
| id | doaj.art-42a6053e16b2492d9151fe8537888822 |
| institution | Directory Open Access Journal |
| issn | 2590-0986 |
| language | English |
| last_indexed | 2024-12-12T17:39:40Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Medicine in Drug Discovery |
| spelling | doaj.art-42a6053e16b2492d9151fe85378888222022-12-22T00:17:06ZengElsevierMedicine in Drug Discovery2590-09862020-09-017100050Simultaneous determination of minoxidil and minoxidil sulfate by high-performance liquid chromatography with UV-detection and its applicationsMio Ichida0Chiharu Fujita1Rena Sumie2Ryotaro Miyano3Hajime Inoue4Division of Regenerative Therapy (Endowed Chair by ANGFA Co.), Department of Plastic Surgery, St. Marianna University School of Medicine, Kawasaki 216-8511, JapanDivision of Regenerative Therapy (Endowed Chair by ANGFA Co.), Department of Plastic Surgery, St. Marianna University School of Medicine, Kawasaki 216-8511, Japan; Laboratory of Cell Applied Technologies Co., Kawasaki 216-8511, JapanDepartment of Plastic Surgery, St. Marianna University School of Medicine, Kawasaki 216-8511, JapanDepartment of Plastic Surgery, St. Marianna University School of Medicine, Kawasaki 216-8511, JapanDivision of Regenerative Therapy (Endowed Chair by ANGFA Co.), Department of Plastic Surgery, St. Marianna University School of Medicine, Kawasaki 216-8511, Japan; Laboratory of Cell Applied Technologies Co., Kawasaki 216-8511, Japan; Corresponding author at: Division of Regenerative Therapy (Endowed Chair ANGFA Co.), Department of Plastic Surgery, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae, Kawasaki 216-8511, Japan.Aims: Minoxidil is a hair growth drug for treating androgenetic alopecia. Although minoxidil is generally administered as a topical formulation, this prodrug must be converted to its active form (minoxidil sulfate) by sulfotransferase in hair follicle tissue. Therefore, its effect may be affected by the sulfureting of minoxidil and de-sulfureting of minoxidil sulfate in hair follicles. To investigate the biotransformation of minoxidil sulfate to minoxidil in hair follicle component cells, a method for simultaneous determination of minoxidil and minoxidil sulfate by high-performance liquid chromatography with UV-detection was established. Main methods: Minoxidil and minoxidil sulfate were separated by a reversed-phase chromatography. Minoxidil sulfate was incubated with human hair follicle keratinocyte- and dermal papilla cell-derived arylsulfatases as well as snail-derived sulfatase. The enzyme mixture was applied on HPLC system directly. Key findings: Minoxidil and minoxidil sulfate were separated simultaneously. The limit of detection of minoxidil and minoxidil sulfate was 25 and 100 pg inj−1, respectively. Snail-derived sulfatase as well as human hair follicle keratinocyte- and dermal papilla cell-derived arylsulfatases hydrolyzed minoxidil sulfate to minoxidil. Arylsulfatase in hair follicle metabolized the active form of minoxidil to the inactive form. Inactivation of active minoxidil (sulfate) was inhibited by the natural substrate of arylsulfatase A, ascorbate-2-sulfate. Significance: Arylsulfatase inhibitors may sustain the effect of minoxidil sulfate in AGA therapy. The developed technique was effective for studies of minoxidil metabolism and bioavailability.http://www.sciencedirect.com/science/article/pii/S2590098620300373MinoxidilMinoxidil sulfateArylsulfataseArylsulfotransferaseBioavailability |
| spellingShingle | Mio Ichida Chiharu Fujita Rena Sumie Ryotaro Miyano Hajime Inoue Simultaneous determination of minoxidil and minoxidil sulfate by high-performance liquid chromatography with UV-detection and its applications Medicine in Drug Discovery Minoxidil Minoxidil sulfate Arylsulfatase Arylsulfotransferase Bioavailability |
| title | Simultaneous determination of minoxidil and minoxidil sulfate by high-performance liquid chromatography with UV-detection and its applications |
| title_full | Simultaneous determination of minoxidil and minoxidil sulfate by high-performance liquid chromatography with UV-detection and its applications |
| title_fullStr | Simultaneous determination of minoxidil and minoxidil sulfate by high-performance liquid chromatography with UV-detection and its applications |
| title_full_unstemmed | Simultaneous determination of minoxidil and minoxidil sulfate by high-performance liquid chromatography with UV-detection and its applications |
| title_short | Simultaneous determination of minoxidil and minoxidil sulfate by high-performance liquid chromatography with UV-detection and its applications |
| title_sort | simultaneous determination of minoxidil and minoxidil sulfate by high performance liquid chromatography with uv detection and its applications |
| topic | Minoxidil Minoxidil sulfate Arylsulfatase Arylsulfotransferase Bioavailability |
| url | http://www.sciencedirect.com/science/article/pii/S2590098620300373 |
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