Gene expression results in lipopolysaccharide-stimulated monocytes depend significantly on the choice of reference genes

Gene expression results in lipopolysaccharide-stimulated monocytes depend significantly on the choice of reference genes

<p>Abstract</p> <p>Background</p> <p>Gene expression in lipopolysaccharide (LPS)-stimulated monocytes is mainly studied by quantitative real-time reverse transcription PCR (RT-qPCR) using GAPDH (glyceraldehyde 3-phosphate dehydrogenase) or ACTB (beta-actin) as reference...

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Bibliographic Details
Main Authors: Øvstebø Reidun, Grimholt Runa M, Piehler Armin P, Berg Jens P
Format: Article
Language:English
Published: BMC 2010-05-01
Series:BMC Immunology
Online Access:http://www.biomedcentral.com/1471-2172/11/21

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