Evaluating the Effectiveness of Screened Lactic Acid Bacteria in Improving Crop Residues Silage: Fermentation Parameter, Nitrogen Fraction, and Bacterial Community
Ensiling characteristics of sweet potato vine (SPV) and peanut straw (PS), as well as the effects of lactic acid bacteria (LAB) strains, Lactococcus Lactis MK524164 (LL) and Lactobacillus farciminis MK524159 (LF), were investigated in this study. Fermentation parameters, nitrogen fractions, and bact...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.680988/full |
| _version_ | 1828553898805166080 |
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| author | Liwen He Yimin Wang Xiang Guo Xiaoyang Chen Qing Zhang |
| author_facet | Liwen He Yimin Wang Xiang Guo Xiaoyang Chen Qing Zhang |
| author_sort | Liwen He |
| collection | DOAJ |
| description | Ensiling characteristics of sweet potato vine (SPV) and peanut straw (PS), as well as the effects of lactic acid bacteria (LAB) strains, Lactococcus Lactis MK524164 (LL) and Lactobacillus farciminis MK524159 (LF), were investigated in this study. Fermentation parameters, nitrogen fractions, and bacterial community of SPV and PS were monitored at intervals during the ensiling process. The results showed that inoculating LAB increased lactate production (2.23 vs. 2.73%; 0.42 vs. 1.67% DM), accelerated pH decline (5.20 vs. 4.47; 6.30 vs. 5.35), and decreased butyrate (0.36% DM vs. not detected), ammonia-N (6.41 vs. 4.18% CP), or nonprotein-N (43.67 vs. 35.82% CP). Meanwhile, it altered the silage bacterial community, where the relative abundance of Lactobacillus was increased (6.67–32.03 vs. 45.27–68.43%; 0.53–10.45 vs. 38.37–68.62%) and that of undesirable bacteria such as Clostridium, Enterobacter, Methylobacterium, or Sphingomonas was much decreased. It is suggested that the screened LAB strains LL and LF can effectively improve the silage quality of SPV and PS silages. |
| first_indexed | 2024-12-12T05:26:36Z |
| format | Article |
| id | doaj.art-42b754c9734b405dba8af3c54b3385fb |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T05:26:36Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-42b754c9734b405dba8af3c54b3385fb2022-12-22T00:36:26ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-05-011310.3389/fmicb.2022.680988680988Evaluating the Effectiveness of Screened Lactic Acid Bacteria in Improving Crop Residues Silage: Fermentation Parameter, Nitrogen Fraction, and Bacterial CommunityLiwen He0Yimin Wang1Xiang Guo2Xiaoyang Chen3Qing Zhang4State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaCollege of Forestry and Landscape Architecture, Guangdong Province Research Center of Woody Forage Engineering Technology, Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, ChinaCollege of Forestry and Landscape Architecture, Guangdong Province Research Center of Woody Forage Engineering Technology, Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, ChinaCollege of Forestry and Landscape Architecture, Guangdong Province Research Center of Woody Forage Engineering Technology, Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, ChinaEnsiling characteristics of sweet potato vine (SPV) and peanut straw (PS), as well as the effects of lactic acid bacteria (LAB) strains, Lactococcus Lactis MK524164 (LL) and Lactobacillus farciminis MK524159 (LF), were investigated in this study. Fermentation parameters, nitrogen fractions, and bacterial community of SPV and PS were monitored at intervals during the ensiling process. The results showed that inoculating LAB increased lactate production (2.23 vs. 2.73%; 0.42 vs. 1.67% DM), accelerated pH decline (5.20 vs. 4.47; 6.30 vs. 5.35), and decreased butyrate (0.36% DM vs. not detected), ammonia-N (6.41 vs. 4.18% CP), or nonprotein-N (43.67 vs. 35.82% CP). Meanwhile, it altered the silage bacterial community, where the relative abundance of Lactobacillus was increased (6.67–32.03 vs. 45.27–68.43%; 0.53–10.45 vs. 38.37–68.62%) and that of undesirable bacteria such as Clostridium, Enterobacter, Methylobacterium, or Sphingomonas was much decreased. It is suggested that the screened LAB strains LL and LF can effectively improve the silage quality of SPV and PS silages.https://www.frontiersin.org/articles/10.3389/fmicb.2022.680988/fullbacterial communitylactic acid bacteriapeanut strawsilage qualitysweet potato vine |
| spellingShingle | Liwen He Yimin Wang Xiang Guo Xiaoyang Chen Qing Zhang Evaluating the Effectiveness of Screened Lactic Acid Bacteria in Improving Crop Residues Silage: Fermentation Parameter, Nitrogen Fraction, and Bacterial Community Frontiers in Microbiology bacterial community lactic acid bacteria peanut straw silage quality sweet potato vine |
| title | Evaluating the Effectiveness of Screened Lactic Acid Bacteria in Improving Crop Residues Silage: Fermentation Parameter, Nitrogen Fraction, and Bacterial Community |
| title_full | Evaluating the Effectiveness of Screened Lactic Acid Bacteria in Improving Crop Residues Silage: Fermentation Parameter, Nitrogen Fraction, and Bacterial Community |
| title_fullStr | Evaluating the Effectiveness of Screened Lactic Acid Bacteria in Improving Crop Residues Silage: Fermentation Parameter, Nitrogen Fraction, and Bacterial Community |
| title_full_unstemmed | Evaluating the Effectiveness of Screened Lactic Acid Bacteria in Improving Crop Residues Silage: Fermentation Parameter, Nitrogen Fraction, and Bacterial Community |
| title_short | Evaluating the Effectiveness of Screened Lactic Acid Bacteria in Improving Crop Residues Silage: Fermentation Parameter, Nitrogen Fraction, and Bacterial Community |
| title_sort | evaluating the effectiveness of screened lactic acid bacteria in improving crop residues silage fermentation parameter nitrogen fraction and bacterial community |
| topic | bacterial community lactic acid bacteria peanut straw silage quality sweet potato vine |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.680988/full |
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