Antagonistic fluorescent Pseudomonads: rhizobacteria with suppressive and plant growth promoting properties against Phytophthora colocasiae, the causal agent of taro leaf blight
Taro leaf blight caused by Phytophthora colocasiae affects plant health and is a major threat to taro culture in Cameroon. Chemical fertilizers used often harm the ecosystem. Plant growth-promoting rhizobacteria (PGPR) are better alternatives that increase plant growth promotion and suppress phytopa...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Polish Academy of Sciences
2023-09-01
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| Series: | Journal of Plant Protection Research |
| Subjects: | |
| Online Access: | https://journals.pan.pl/Content/128572/PDF/OA_08_JPPR_63_3_1625_Arsene.pdf |
| _version_ | 1827814214602850304 |
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| author | Samuel Arsène Ntyam Mendo Dorice Nguelo Dzumafo Laure Brigitte Kouitcheu Mabeku Severin Tchameni Nguemezi Lambert Sameza Modeste Rosalie Anne Ngono Ngane |
| author_facet | Samuel Arsène Ntyam Mendo Dorice Nguelo Dzumafo Laure Brigitte Kouitcheu Mabeku Severin Tchameni Nguemezi Lambert Sameza Modeste Rosalie Anne Ngono Ngane |
| author_sort | Samuel Arsène Ntyam Mendo |
| collection | DOAJ |
| description | Taro leaf blight caused by Phytophthora colocasiae affects plant health and is a major threat to taro culture in Cameroon. Chemical fertilizers used often harm the ecosystem. Plant growth-promoting rhizobacteria (PGPR) are better alternatives that increase plant growth promotion and suppress phytopathogens. In the present study, a total of 67 fluorescent Pseudomonas spp. was characterized by 17.91, 5.97, and 4.47% populations of P. fluorescens, P. chlororaphis, and P. putida, respectively, among the most represented. More than 36% of bacteria showed antagonistic potential through the production of both diffusible and volatile compounds. Some of them (03) exhibited antagonistic activity in dual culture against P. colocasiae with a diameter greater than 13 mm. These rhizobacteria produced a significant amount of siderophore, IAA, SA, HCN, protease, lipases, and cellulases. For the pot experiment, treatment by Pseudomonas significantly increased the enzymatic activity involved in the resistance of taro, such as peroxidase (PO), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL). The two antagonists also increased plant growth parameters of taro such as chlorophyll, plant height, shoot length, total leaf surface, fresh root biomass, and fresh leaf biomass. These findings showed that fluorescent Pseudomonas have an intriguing and undeniable potential in the fight against P. colocasiae, which could lead to the development of a biopesticide in the future. |
| first_indexed | 2024-03-11T23:42:47Z |
| format | Article |
| id | doaj.art-42b874a94ee34c2a91557f857fdbc480 |
| institution | Directory Open Access Journal |
| issn | 1427-4345 1899-007X |
| language | English |
| last_indexed | 2024-03-11T23:42:47Z |
| publishDate | 2023-09-01 |
| publisher | Polish Academy of Sciences |
| record_format | Article |
| series | Journal of Plant Protection Research |
| spelling | doaj.art-42b874a94ee34c2a91557f857fdbc4802023-09-19T15:19:53ZengPolish Academy of SciencesJournal of Plant Protection Research1427-43451899-007X2023-09-01vol. 63No 3https://doi.org/10.24425/jppr.2023.146875Antagonistic fluorescent Pseudomonads: rhizobacteria with suppressive and plant growth promoting properties against Phytophthora colocasiae, the causal agent of taro leaf blightSamuel Arsène Ntyam Mendo0https://orcid.org/0000-0002-9849-3098Dorice Nguelo Dzumafo1Laure Brigitte Kouitcheu Mabeku2Severin Tchameni Nguemezi3Lambert Sameza Modeste4Rosalie Anne Ngono Ngane5Department of Biological Sciences, Higher Teacher Training College, University of Yaounde 1, Yaounde, CameroonDepartment of Biochemistry, Faculty of Sciences, University of Douala, Douala, CameroonDepartment of Microbiology, Faculty of Science, University of Yaoundé 1, Yaounde, CameroonDepartment of Biochemistry, Faculty of Sciences, University of Douala, Douala, CameroonDepartment of Biochemistry, Faculty of Sciences, University of Douala, Douala, CameroonDepartment of Biochemistry, Faculty of Sciences, University of Douala, Douala, CameroonTaro leaf blight caused by Phytophthora colocasiae affects plant health and is a major threat to taro culture in Cameroon. Chemical fertilizers used often harm the ecosystem. Plant growth-promoting rhizobacteria (PGPR) are better alternatives that increase plant growth promotion and suppress phytopathogens. In the present study, a total of 67 fluorescent Pseudomonas spp. was characterized by 17.91, 5.97, and 4.47% populations of P. fluorescens, P. chlororaphis, and P. putida, respectively, among the most represented. More than 36% of bacteria showed antagonistic potential through the production of both diffusible and volatile compounds. Some of them (03) exhibited antagonistic activity in dual culture against P. colocasiae with a diameter greater than 13 mm. These rhizobacteria produced a significant amount of siderophore, IAA, SA, HCN, protease, lipases, and cellulases. For the pot experiment, treatment by Pseudomonas significantly increased the enzymatic activity involved in the resistance of taro, such as peroxidase (PO), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL). The two antagonists also increased plant growth parameters of taro such as chlorophyll, plant height, shoot length, total leaf surface, fresh root biomass, and fresh leaf biomass. These findings showed that fluorescent Pseudomonas have an intriguing and undeniable potential in the fight against P. colocasiae, which could lead to the development of a biopesticide in the future.https://journals.pan.pl/Content/128572/PDF/OA_08_JPPR_63_3_1625_Arsene.pdfbiocontrolfluorescent pseudomonasphytophthora colocasiaerhizobacteriataro |
| spellingShingle | Samuel Arsène Ntyam Mendo Dorice Nguelo Dzumafo Laure Brigitte Kouitcheu Mabeku Severin Tchameni Nguemezi Lambert Sameza Modeste Rosalie Anne Ngono Ngane Antagonistic fluorescent Pseudomonads: rhizobacteria with suppressive and plant growth promoting properties against Phytophthora colocasiae, the causal agent of taro leaf blight Journal of Plant Protection Research biocontrol fluorescent pseudomonas phytophthora colocasiae rhizobacteria taro |
| title | Antagonistic fluorescent Pseudomonads: rhizobacteria with suppressive and plant growth promoting properties against Phytophthora colocasiae, the causal agent of taro leaf blight |
| title_full | Antagonistic fluorescent Pseudomonads: rhizobacteria with suppressive and plant growth promoting properties against Phytophthora colocasiae, the causal agent of taro leaf blight |
| title_fullStr | Antagonistic fluorescent Pseudomonads: rhizobacteria with suppressive and plant growth promoting properties against Phytophthora colocasiae, the causal agent of taro leaf blight |
| title_full_unstemmed | Antagonistic fluorescent Pseudomonads: rhizobacteria with suppressive and plant growth promoting properties against Phytophthora colocasiae, the causal agent of taro leaf blight |
| title_short | Antagonistic fluorescent Pseudomonads: rhizobacteria with suppressive and plant growth promoting properties against Phytophthora colocasiae, the causal agent of taro leaf blight |
| title_sort | antagonistic fluorescent pseudomonads rhizobacteria with suppressive and plant growth promoting properties against phytophthora colocasiae the causal agent of taro leaf blight |
| topic | biocontrol fluorescent pseudomonas phytophthora colocasiae rhizobacteria taro |
| url | https://journals.pan.pl/Content/128572/PDF/OA_08_JPPR_63_3_1625_Arsene.pdf |
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