A new Eimeria coccidian species (Apicomplexa: Eimeriidae) from Père David’s deer (Elaphurus davidianus Milne-Edwards, 1866) in Dafeng Milu National Nature Reserve in Jiangsu Province, eastern China
Abstract Background Eimeria coccidiosis is a significant intestinal parasitic disease, which can lead to weight loss, disease and even death of many animals. At present, there is no information about the prevalence of Eimeria among the world’s endangered species of Père David’s deer (Elaphurus david...
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| Format: | Article |
| Language: | English |
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BMC
2022-06-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12917-022-03308-2 |
| _version_ | 1811257003900665856 |
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| author | Weimin Cai Zeyang Suding Lele Wang Zhaofeng Hou Dandan Liu Siyang Huang Jinjun Xu Jianping Tao |
| author_facet | Weimin Cai Zeyang Suding Lele Wang Zhaofeng Hou Dandan Liu Siyang Huang Jinjun Xu Jianping Tao |
| author_sort | Weimin Cai |
| collection | DOAJ |
| description | Abstract Background Eimeria coccidiosis is a significant intestinal parasitic disease, which can lead to weight loss, disease and even death of many animals. At present, there is no information about the prevalence of Eimeria among the world’s endangered species of Père David’s deer (Elaphurus davidianus). Therefore, the purpose of this study is to identify an unknown Eimeria genus in the Père David’s deer in Dafeng Milu National Nature Reserve, China. Results A new Eimeria species is described from Père David’s deer. Sporulated oocysts (n = 54) are pyriform, with a rough, yellowish brown, 2-layered oocyst wall (2.5 μm thick). A numerous small granules are dispersed randomly on the wall. Oocysts measured 41.2 (39.2–42.8) μm × 29.5 (27.9–30.5) μm, oocyst length/width (L/W) ratio, 1.4. Oocyst residuum, a polar granule and a polar cap are absent. The micropyle (3.5 μm wide) is present. Sporocysts are spindle shaped, 18.2 (16.5–20.0) μm × 10.5 (9.8–11.9) μm, sporocyst L/W ratio, 1.7 (1.5–1.9). A thin convex Stieda body is present and the sporocyst residuum is composed of numerous small granules less than 2.0 μm in diameter dispersed randomly. Each sporocyst contained 2 comma-shaped sporozoites in head-to-tail arrangement. A nucleus is located immediately anterior to the posterior, strong refractive and subspherical refractile body (~ 8 μm). Molecular analysis was conducted at the 18S, ITS-1 and COI loci. Conclusion Based on the morphological and molecular data, this isolate is a new species of coccidian parasite, which is named Eimeria davidianusi after its host, the Père David’s deer (Elaphurus davidianus). |
| first_indexed | 2024-04-12T17:49:43Z |
| format | Article |
| id | doaj.art-42bc0e0b671949bbabbecafdd0610d7b |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-12T17:49:43Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-42bc0e0b671949bbabbecafdd0610d7b2022-12-22T03:22:32ZengBMCBMC Veterinary Research1746-61482022-06-0118111210.1186/s12917-022-03308-2A new Eimeria coccidian species (Apicomplexa: Eimeriidae) from Père David’s deer (Elaphurus davidianus Milne-Edwards, 1866) in Dafeng Milu National Nature Reserve in Jiangsu Province, eastern ChinaWeimin Cai0Zeyang Suding1Lele Wang2Zhaofeng Hou3Dandan Liu4Siyang Huang5Jinjun Xu6Jianping Tao7College of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityAbstract Background Eimeria coccidiosis is a significant intestinal parasitic disease, which can lead to weight loss, disease and even death of many animals. At present, there is no information about the prevalence of Eimeria among the world’s endangered species of Père David’s deer (Elaphurus davidianus). Therefore, the purpose of this study is to identify an unknown Eimeria genus in the Père David’s deer in Dafeng Milu National Nature Reserve, China. Results A new Eimeria species is described from Père David’s deer. Sporulated oocysts (n = 54) are pyriform, with a rough, yellowish brown, 2-layered oocyst wall (2.5 μm thick). A numerous small granules are dispersed randomly on the wall. Oocysts measured 41.2 (39.2–42.8) μm × 29.5 (27.9–30.5) μm, oocyst length/width (L/W) ratio, 1.4. Oocyst residuum, a polar granule and a polar cap are absent. The micropyle (3.5 μm wide) is present. Sporocysts are spindle shaped, 18.2 (16.5–20.0) μm × 10.5 (9.8–11.9) μm, sporocyst L/W ratio, 1.7 (1.5–1.9). A thin convex Stieda body is present and the sporocyst residuum is composed of numerous small granules less than 2.0 μm in diameter dispersed randomly. Each sporocyst contained 2 comma-shaped sporozoites in head-to-tail arrangement. A nucleus is located immediately anterior to the posterior, strong refractive and subspherical refractile body (~ 8 μm). Molecular analysis was conducted at the 18S, ITS-1 and COI loci. Conclusion Based on the morphological and molecular data, this isolate is a new species of coccidian parasite, which is named Eimeria davidianusi after its host, the Père David’s deer (Elaphurus davidianus).https://doi.org/10.1186/s12917-022-03308-2Père David’s deerMorphologyEimeria davidianusiPhylogenetics |
| spellingShingle | Weimin Cai Zeyang Suding Lele Wang Zhaofeng Hou Dandan Liu Siyang Huang Jinjun Xu Jianping Tao A new Eimeria coccidian species (Apicomplexa: Eimeriidae) from Père David’s deer (Elaphurus davidianus Milne-Edwards, 1866) in Dafeng Milu National Nature Reserve in Jiangsu Province, eastern China BMC Veterinary Research Père David’s deer Morphology Eimeria davidianusi Phylogenetics |
| title | A new Eimeria coccidian species (Apicomplexa: Eimeriidae) from Père David’s deer (Elaphurus davidianus Milne-Edwards, 1866) in Dafeng Milu National Nature Reserve in Jiangsu Province, eastern China |
| title_full | A new Eimeria coccidian species (Apicomplexa: Eimeriidae) from Père David’s deer (Elaphurus davidianus Milne-Edwards, 1866) in Dafeng Milu National Nature Reserve in Jiangsu Province, eastern China |
| title_fullStr | A new Eimeria coccidian species (Apicomplexa: Eimeriidae) from Père David’s deer (Elaphurus davidianus Milne-Edwards, 1866) in Dafeng Milu National Nature Reserve in Jiangsu Province, eastern China |
| title_full_unstemmed | A new Eimeria coccidian species (Apicomplexa: Eimeriidae) from Père David’s deer (Elaphurus davidianus Milne-Edwards, 1866) in Dafeng Milu National Nature Reserve in Jiangsu Province, eastern China |
| title_short | A new Eimeria coccidian species (Apicomplexa: Eimeriidae) from Père David’s deer (Elaphurus davidianus Milne-Edwards, 1866) in Dafeng Milu National Nature Reserve in Jiangsu Province, eastern China |
| title_sort | new eimeria coccidian species apicomplexa eimeriidae from pere david s deer elaphurus davidianus milne edwards 1866 in dafeng milu national nature reserve in jiangsu province eastern china |
| topic | Père David’s deer Morphology Eimeria davidianusi Phylogenetics |
| url | https://doi.org/10.1186/s12917-022-03308-2 |
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