Plasmid pND6-1 enhances the stability and conjugative transfer of co-resident companion plasmid pND6-2 in the naphthalene-degradative Pseudomonas putida strain ND6
Background: Bacterial cells often harbor multiple plasmids, but the interaction between these plasmids is poorly understood. Pseudomonas putida ND6, one of the typical naphthalene-degrading bacteria, contains two annotated plasmids, pND6-1 and pND6-2. The conjugative plasmid pND6-2 can be used as a...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Electronic Journal of Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345822000318 |
| _version_ | 1811273843805782016 |
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| author | Shan Wang Mingming Liu Dan Wang Shanshan Li Wei Yan |
| author_facet | Shan Wang Mingming Liu Dan Wang Shanshan Li Wei Yan |
| author_sort | Shan Wang |
| collection | DOAJ |
| description | Background: Bacterial cells often harbor multiple plasmids, but the interaction between these plasmids is poorly understood. Pseudomonas putida ND6, one of the typical naphthalene-degrading bacteria, contains two annotated plasmids, pND6-1 and pND6-2. The conjugative plasmid pND6-2 can be used as a helper to mobilize the naphthalene-degrading plasmid pND6-1. The role of pND6-1 in functions other than naphthalene degradation has never been explored. Therefore, it is of great significance to study the possible effect of pND6-1 on the transfer rate or maintenance of pND6-2. Results: To investigate the interaction between pND6-1 and pND6-2, we successfully cured plasmid pND6-1 using the incompatibility method, generated the pND6-1-null strain ND6dp1, and first classified it into the IncP-7 group by curing experimental evidence. Subsequently, the pND6-1-cured mutant showed an insignificant increase in cell growth compared with the wild ND6, indicating that a lower cost was imposed on the host by pND6-1. The influences of pND6-1 on the stability and conjugation of plasmid pND6-2 were subsequently explored. The results showed that the presence of pND6-1 enhanced the stability of pND6-2 in natural host ND6 and heterologous host P. putida KT2440. In addition, the presence of pND6-1 in wild-type hosts promoted intra-specific and inter-specific conjugative transfer efficiency of pND6-2 by 3.9–6.3-fold with P. putida, P. resinovans, and P. fluorescens as the recipients, respectively. Conclusions: Collectively, these results suggest that the plasmid pND6-1 improves the pND6-2 plasmid's stability and transfer rate in the ND6 strain with the selected recipient strains.How to cite: Wang S, Liu M, Wang D, et al. Plasmid pND6-1 enhances the stability and conjugative transfer of co-resident companion plasmid pND6-2 in the naphthalene-degradative Pseudomonas putida strain ND6. Electron J Biotechnol 2022;59. https://doi.org/10.1016/j.ejbt.2022.08.002. |
| first_indexed | 2024-04-12T23:06:50Z |
| format | Article |
| id | doaj.art-42bd41e5bc0d466cad6006aff3c06c30 |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-04-12T23:06:50Z |
| publishDate | 2022-09-01 |
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| spelling | doaj.art-42bd41e5bc0d466cad6006aff3c06c302022-12-22T03:12:53ZengElsevierElectronic Journal of Biotechnology0717-34582022-09-01597482Plasmid pND6-1 enhances the stability and conjugative transfer of co-resident companion plasmid pND6-2 in the naphthalene-degradative Pseudomonas putida strain ND6Shan Wang0Mingming Liu1Dan Wang2Shanshan Li3Wei Yan4Xi’an Key Laboratory of Solid Waste Recycling and Resource Recovery, Department of Environmental Engineering, Xi’an Jiaotong University, Xi’an 710049, China; Key Laboratory of Coal Clean Conversion & Chemical Engineering Process, Xinjiang Uygur Autonomous Region, College of Chemical Engineering, Xinjiang University, Urumqi 830046, ChinaXi’an Key Laboratory of Solid Waste Recycling and Resource Recovery, Department of Environmental Engineering, Xi’an Jiaotong University, Xi’an 710049, ChinaXi’an Key Laboratory of Solid Waste Recycling and Resource Recovery, Department of Environmental Engineering, Xi’an Jiaotong University, Xi’an 710049, ChinaXi’an Key Laboratory of Solid Waste Recycling and Resource Recovery, Department of Environmental Engineering, Xi’an Jiaotong University, Xi’an 710049, China; Corresponding author.Xi’an Key Laboratory of Solid Waste Recycling and Resource Recovery, Department of Environmental Engineering, Xi’an Jiaotong University, Xi’an 710049, ChinaBackground: Bacterial cells often harbor multiple plasmids, but the interaction between these plasmids is poorly understood. Pseudomonas putida ND6, one of the typical naphthalene-degrading bacteria, contains two annotated plasmids, pND6-1 and pND6-2. The conjugative plasmid pND6-2 can be used as a helper to mobilize the naphthalene-degrading plasmid pND6-1. The role of pND6-1 in functions other than naphthalene degradation has never been explored. Therefore, it is of great significance to study the possible effect of pND6-1 on the transfer rate or maintenance of pND6-2. Results: To investigate the interaction between pND6-1 and pND6-2, we successfully cured plasmid pND6-1 using the incompatibility method, generated the pND6-1-null strain ND6dp1, and first classified it into the IncP-7 group by curing experimental evidence. Subsequently, the pND6-1-cured mutant showed an insignificant increase in cell growth compared with the wild ND6, indicating that a lower cost was imposed on the host by pND6-1. The influences of pND6-1 on the stability and conjugation of plasmid pND6-2 were subsequently explored. The results showed that the presence of pND6-1 enhanced the stability of pND6-2 in natural host ND6 and heterologous host P. putida KT2440. In addition, the presence of pND6-1 in wild-type hosts promoted intra-specific and inter-specific conjugative transfer efficiency of pND6-2 by 3.9–6.3-fold with P. putida, P. resinovans, and P. fluorescens as the recipients, respectively. Conclusions: Collectively, these results suggest that the plasmid pND6-1 improves the pND6-2 plasmid's stability and transfer rate in the ND6 strain with the selected recipient strains.How to cite: Wang S, Liu M, Wang D, et al. Plasmid pND6-1 enhances the stability and conjugative transfer of co-resident companion plasmid pND6-2 in the naphthalene-degradative Pseudomonas putida strain ND6. Electron J Biotechnol 2022;59. https://doi.org/10.1016/j.ejbt.2022.08.002.http://www.sciencedirect.com/science/article/pii/S0717345822000318Conjugative plasmidConjugative transferCo-resident plasmidNaphthalene degrading bacteriaPlasmidPlasmid curing |
| spellingShingle | Shan Wang Mingming Liu Dan Wang Shanshan Li Wei Yan Plasmid pND6-1 enhances the stability and conjugative transfer of co-resident companion plasmid pND6-2 in the naphthalene-degradative Pseudomonas putida strain ND6 Electronic Journal of Biotechnology Conjugative plasmid Conjugative transfer Co-resident plasmid Naphthalene degrading bacteria Plasmid Plasmid curing |
| title | Plasmid pND6-1 enhances the stability and conjugative transfer of co-resident companion plasmid pND6-2 in the naphthalene-degradative Pseudomonas putida strain ND6 |
| title_full | Plasmid pND6-1 enhances the stability and conjugative transfer of co-resident companion plasmid pND6-2 in the naphthalene-degradative Pseudomonas putida strain ND6 |
| title_fullStr | Plasmid pND6-1 enhances the stability and conjugative transfer of co-resident companion plasmid pND6-2 in the naphthalene-degradative Pseudomonas putida strain ND6 |
| title_full_unstemmed | Plasmid pND6-1 enhances the stability and conjugative transfer of co-resident companion plasmid pND6-2 in the naphthalene-degradative Pseudomonas putida strain ND6 |
| title_short | Plasmid pND6-1 enhances the stability and conjugative transfer of co-resident companion plasmid pND6-2 in the naphthalene-degradative Pseudomonas putida strain ND6 |
| title_sort | plasmid pnd6 1 enhances the stability and conjugative transfer of co resident companion plasmid pnd6 2 in the naphthalene degradative pseudomonas putida strain nd6 |
| topic | Conjugative plasmid Conjugative transfer Co-resident plasmid Naphthalene degrading bacteria Plasmid Plasmid curing |
| url | http://www.sciencedirect.com/science/article/pii/S0717345822000318 |
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