Immunoselective progression of a multi-epitope-based subunit vaccine candidate to convey protection against the parasite Onchocerca lupi
Globalization has resulted in an increasing worldwide movement of humans and animals as well as changes in the external environment, temperature, and social interactions, resulting in the accelerated emergence of new diseases in humans and dogs, such as Onchocerca lupi-caused ocular onchocerciasis....
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Informatics in Medicine Unlocked |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352914823000515 |
| _version_ | 1797845055254298624 |
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| author | Mohammad Nahian Rahman Shakil Ahmed Mahamudul Hasan Md. Sabbir Ahmed Shuvo Md. Anwarul Islam Rakibul Hasan Shiplu Roy Hemayet Hossain Md. Mukthar Mia |
| author_facet | Mohammad Nahian Rahman Shakil Ahmed Mahamudul Hasan Md. Sabbir Ahmed Shuvo Md. Anwarul Islam Rakibul Hasan Shiplu Roy Hemayet Hossain Md. Mukthar Mia |
| author_sort | Mohammad Nahian Rahman |
| collection | DOAJ |
| description | Globalization has resulted in an increasing worldwide movement of humans and animals as well as changes in the external environment, temperature, and social interactions, resulting in the accelerated emergence of new diseases in humans and dogs, such as Onchocerca lupi-caused ocular onchocerciasis. Presently, zoonotic onchocerciasis affects 15.5 million people globally; however, the lack of effective anthelmintics and vaccinations highlighted the disease as a global public health concern. Therefore, the current study offers computational methods for anticipating and creating a multi-epitope subunit vaccine targeting Onchocerca lupi by utilizing suitable linkers and adjuvants to combine CTL, HTL, and BCL epitopes. Three different vaccines (V1, V2, and V3) were designed using three distinct adjuvants and then the vaccine constructs antigenicity, solubility, and non-allergenicity were determined. Then, the physicochemical characteristics of the constructed vaccine candidates were analyzed, and also the tertiary structure of the vaccines was anticipated, which was later verified and refined. Molecular docking and molecular dynamics simulation results showed that the V1 vaccine constructs expected affinity and binding free energy for the dimeric state of human Toll-like receptor-4 (hTLR-4) were promising. These results indicate that the suggested vaccine candidate could probably enhance the immune response to Onchocerca lupi infection. The results should be confirmed in experimental studies. |
| first_indexed | 2024-04-09T17:32:18Z |
| format | Article |
| id | doaj.art-42bda93cba3742348d3beba5374251e4 |
| institution | Directory Open Access Journal |
| issn | 2352-9148 |
| language | English |
| last_indexed | 2024-04-09T17:32:18Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Informatics in Medicine Unlocked |
| spelling | doaj.art-42bda93cba3742348d3beba5374251e42023-04-18T04:08:54ZengElsevierInformatics in Medicine Unlocked2352-91482023-01-0138101209Immunoselective progression of a multi-epitope-based subunit vaccine candidate to convey protection against the parasite Onchocerca lupiMohammad Nahian Rahman0Shakil Ahmed1Mahamudul Hasan2Md. Sabbir Ahmed Shuvo3Md. Anwarul Islam4Rakibul Hasan5Shiplu Roy6Hemayet Hossain7Md. Mukthar Mia8Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, 3100, Bangladesh; Corresponding author.Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, 3100, Bangladesh; Corresponding author.Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, 3100, BangladeshFaculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, 3100, BangladeshFaculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, 3100, BangladeshFaculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, 3100, BangladeshFaculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, 3100, Bangladesh; Department of Livestock Production and Management, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, 3100, BangladeshFaculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, 3100, BangladeshFaculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, 3100, Bangladesh; Department of Poultry Science, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet, 3100, BangladeshGlobalization has resulted in an increasing worldwide movement of humans and animals as well as changes in the external environment, temperature, and social interactions, resulting in the accelerated emergence of new diseases in humans and dogs, such as Onchocerca lupi-caused ocular onchocerciasis. Presently, zoonotic onchocerciasis affects 15.5 million people globally; however, the lack of effective anthelmintics and vaccinations highlighted the disease as a global public health concern. Therefore, the current study offers computational methods for anticipating and creating a multi-epitope subunit vaccine targeting Onchocerca lupi by utilizing suitable linkers and adjuvants to combine CTL, HTL, and BCL epitopes. Three different vaccines (V1, V2, and V3) were designed using three distinct adjuvants and then the vaccine constructs antigenicity, solubility, and non-allergenicity were determined. Then, the physicochemical characteristics of the constructed vaccine candidates were analyzed, and also the tertiary structure of the vaccines was anticipated, which was later verified and refined. Molecular docking and molecular dynamics simulation results showed that the V1 vaccine constructs expected affinity and binding free energy for the dimeric state of human Toll-like receptor-4 (hTLR-4) were promising. These results indicate that the suggested vaccine candidate could probably enhance the immune response to Onchocerca lupi infection. The results should be confirmed in experimental studies.http://www.sciencedirect.com/science/article/pii/S2352914823000515Onchocerca lupiReverse vaccinologyImmune-informaticsAntigenicityMolecular dockingIn-silico cloning |
| spellingShingle | Mohammad Nahian Rahman Shakil Ahmed Mahamudul Hasan Md. Sabbir Ahmed Shuvo Md. Anwarul Islam Rakibul Hasan Shiplu Roy Hemayet Hossain Md. Mukthar Mia Immunoselective progression of a multi-epitope-based subunit vaccine candidate to convey protection against the parasite Onchocerca lupi Informatics in Medicine Unlocked Onchocerca lupi Reverse vaccinology Immune-informatics Antigenicity Molecular docking In-silico cloning |
| title | Immunoselective progression of a multi-epitope-based subunit vaccine candidate to convey protection against the parasite Onchocerca lupi |
| title_full | Immunoselective progression of a multi-epitope-based subunit vaccine candidate to convey protection against the parasite Onchocerca lupi |
| title_fullStr | Immunoselective progression of a multi-epitope-based subunit vaccine candidate to convey protection against the parasite Onchocerca lupi |
| title_full_unstemmed | Immunoselective progression of a multi-epitope-based subunit vaccine candidate to convey protection against the parasite Onchocerca lupi |
| title_short | Immunoselective progression of a multi-epitope-based subunit vaccine candidate to convey protection against the parasite Onchocerca lupi |
| title_sort | immunoselective progression of a multi epitope based subunit vaccine candidate to convey protection against the parasite onchocerca lupi |
| topic | Onchocerca lupi Reverse vaccinology Immune-informatics Antigenicity Molecular docking In-silico cloning |
| url | http://www.sciencedirect.com/science/article/pii/S2352914823000515 |
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