The invasive and new non-invasive methods of mammalian oocyte and embryo quality assessment: a review
The quality of oocytes-embryos can be determined by several techniques, including morphological, molecular, cellular and biochemical ones. The morphological methods of female gamete or embryo quality assessment often use thе following in vitro manipulation procedures such as: in vitro maturation (IV...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2012-04-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-201204-0001_the-invasive-and-new-non-invasive-methods-of-mammalian-oocyte-and-embryo-quality-assessment-a-review.php |
| _version_ | 1797897577788604416 |
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| author | D. Bukowska B. Kempisty H. Piotrowska R. Walczak P. Sniadek J. Dziuban K.P. Brussow J.M. Jaskowski M. Nowicki |
| author_facet | D. Bukowska B. Kempisty H. Piotrowska R. Walczak P. Sniadek J. Dziuban K.P. Brussow J.M. Jaskowski M. Nowicki |
| author_sort | D. Bukowska |
| collection | DOAJ |
| description | The quality of oocytes-embryos can be determined by several techniques, including morphological, molecular, cellular and biochemical ones. The morphological methods of female gamete or embryo quality assessment often use thе following in vitro manipulation procedures such as: in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro embryo production (IVP). However, these methods are highly subjective and the morphological classification of oocytes or embryos is not always compatible with their ability to grow and develop. Additionally, molecular biology methods are objective and present parametric results, which are more or less comparable to the real oocyte-embryo "health". Although these techniques enable us to determine markers of oocyte-embryo developmental potential, when applied they lead to destruction of the analysed cells. Therefore, the need still exists to search for new methods that will be highly objective (parametric) and, which is most important, non-invasive. In this review, the morphological and molecular methods of oocyte-embryo quality assessment are presented. Moreover, we described a new system based on microfluidic technology (Lab-on-Chip) which allows the creation of a new device for mammalian oocyte as well as embryo quality evaluation: by using their spectral characterisation following embryo transfer (ET) procedures in the cattle and the pig. |
| first_indexed | 2024-04-10T08:00:35Z |
| format | Article |
| id | doaj.art-42c10d027f6340acae78aff84330c128 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T08:00:35Z |
| publishDate | 2012-04-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-42c10d027f6340acae78aff84330c1282023-02-23T03:50:02ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922012-04-0157416917610.17221/5913-VETMEDvet-201204-0001The invasive and new non-invasive methods of mammalian oocyte and embryo quality assessment: a reviewD. Bukowska0B. Kempisty1H. Piotrowska2R. Walczak3P. Sniadek4J. Dziuban5K.P. Brussow6J.M. Jaskowski7M. Nowicki8Poznan University of Life Sciences, Poznan, PolandPoznan University of Medical Sciences, Poznan, PolandPoznan University of Medical Sciences, Poznan, PolandWroclaw University of Technology, Wroclaw, PolandWroclaw University of Technology, Wroclaw, PolandWroclaw University of Technology, Wroclaw, PolandLeibniz Institute for Farm Animal Biology, Dummerstorf, GermanyPoznan University of Life Sciences, Poznan, PolandPoznan University of Medical Sciences, Poznan, PolandThe quality of oocytes-embryos can be determined by several techniques, including morphological, molecular, cellular and biochemical ones. The morphological methods of female gamete or embryo quality assessment often use thе following in vitro manipulation procedures such as: in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro embryo production (IVP). However, these methods are highly subjective and the morphological classification of oocytes or embryos is not always compatible with their ability to grow and develop. Additionally, molecular biology methods are objective and present parametric results, which are more or less comparable to the real oocyte-embryo "health". Although these techniques enable us to determine markers of oocyte-embryo developmental potential, when applied they lead to destruction of the analysed cells. Therefore, the need still exists to search for new methods that will be highly objective (parametric) and, which is most important, non-invasive. In this review, the morphological and molecular methods of oocyte-embryo quality assessment are presented. Moreover, we described a new system based on microfluidic technology (Lab-on-Chip) which allows the creation of a new device for mammalian oocyte as well as embryo quality evaluation: by using their spectral characterisation following embryo transfer (ET) procedures in the cattle and the pig.https://vetmed.agriculturejournals.cz/artkey/vet-201204-0001_the-invasive-and-new-non-invasive-methods-of-mammalian-oocyte-and-embryo-quality-assessment-a-review.phpoocyteembryoqualitymicrofluidics |
| spellingShingle | D. Bukowska B. Kempisty H. Piotrowska R. Walczak P. Sniadek J. Dziuban K.P. Brussow J.M. Jaskowski M. Nowicki The invasive and new non-invasive methods of mammalian oocyte and embryo quality assessment: a review Veterinární Medicína oocyte embryo quality microfluidics |
| title | The invasive and new non-invasive methods of mammalian oocyte and embryo quality assessment: a review |
| title_full | The invasive and new non-invasive methods of mammalian oocyte and embryo quality assessment: a review |
| title_fullStr | The invasive and new non-invasive methods of mammalian oocyte and embryo quality assessment: a review |
| title_full_unstemmed | The invasive and new non-invasive methods of mammalian oocyte and embryo quality assessment: a review |
| title_short | The invasive and new non-invasive methods of mammalian oocyte and embryo quality assessment: a review |
| title_sort | invasive and new non invasive methods of mammalian oocyte and embryo quality assessment a review |
| topic | oocyte embryo quality microfluidics |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-201204-0001_the-invasive-and-new-non-invasive-methods-of-mammalian-oocyte-and-embryo-quality-assessment-a-review.php |
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