Mesenchymal Stem Cell Transplantation Increases Antioxidant Protein Expression and Ameliorates GP91/ROS/Inflammasome Signals in Diabetic Cardiomyopathy
Background: Cardiomyopathy is one of the complications associated with diabetes. Due to its high prevalence, diabetic cardiomyopathy has become an urgent issue for diabetic patients. Various pathological signals are related to diabetic cardiomyopathy progress, including inflammasome. Mesenchymal ste...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Journal of Cardiovascular Development and Disease |
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| Online Access: | https://www.mdpi.com/2308-3425/9/11/381 |
| _version_ | 1797467774680825856 |
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| author | Wei-Syun Hu Tung-Sheng Chen Ka-Hung Cheang Wei-Yu Liao Chin-Hsien Chang |
| author_facet | Wei-Syun Hu Tung-Sheng Chen Ka-Hung Cheang Wei-Yu Liao Chin-Hsien Chang |
| author_sort | Wei-Syun Hu |
| collection | DOAJ |
| description | Background: Cardiomyopathy is one of the complications associated with diabetes. Due to its high prevalence, diabetic cardiomyopathy has become an urgent issue for diabetic patients. Various pathological signals are related to diabetic cardiomyopathy progress, including inflammasome. Mesenchymal stem cell transplantation is full of potential for the treatment of diabetic cardiomyopathy because of stem cell cardiac regenerative capability. This study investigates whether mesenchymal stem cell transplantation shows therapeutic effects on diabetic cardiomyopathy through inflammasome signaling regulation. Methods: Wistar male rats were divided into three groups including Sham, T1DM (rats with type 1 diabetes) and T1DM + WJSC (T1DM rats receiving 1 × 10<sup>6</sup> stem cells per rat). Results: Compared to the Sham, experimental results indicated that several pathological conditions can be observed in heart tissues with T1DM, including structural change, fibrosis, oxidative stress elevation and inflammasome related protein expression. All of these pathological conditions were significantly improved in T1DM rats receiving mesenchymal stem cell transplantation (T1DM + WJSC). Furthermore, the experimental findings suggest that mesenchymal stem cell transplantation exerted antioxidant protein expression in diabetic heart tissues, resulting in a decrease in oxidative stress and inflammasome signaling blockage. Conclusion: These findings imply that mesenchymal stem cell transplantation shows therapeutic effects on diabetic cardiomyopathy through inflammasome regulation induced by oxidative stress. |
| first_indexed | 2024-03-09T18:58:30Z |
| format | Article |
| id | doaj.art-42c3b48fa26d4eca88f555b16e68c626 |
| institution | Directory Open Access Journal |
| issn | 2308-3425 |
| language | English |
| last_indexed | 2024-03-09T18:58:30Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Cardiovascular Development and Disease |
| spelling | doaj.art-42c3b48fa26d4eca88f555b16e68c6262023-11-24T05:14:12ZengMDPI AGJournal of Cardiovascular Development and Disease2308-34252022-11-0191138110.3390/jcdd9110381Mesenchymal Stem Cell Transplantation Increases Antioxidant Protein Expression and Ameliorates GP91/ROS/Inflammasome Signals in Diabetic CardiomyopathyWei-Syun Hu0Tung-Sheng Chen1Ka-Hung Cheang2Wei-Yu Liao3Chin-Hsien Chang4School of Medicine, College of Medicine, China Medical University, Taichung 40042, TaiwanGraduate Program of Biotechnology and Pharmaceutical Industries, National Taiwan Normal University, Taipei 11677, TaiwanGraduate Program of Biotechnology and Pharmaceutical Industries, National Taiwan Normal University, Taipei 11677, TaiwanTraditional Chinese Medicine Department, En Chu Kong Hospital, New Taipei City 40237, TaiwanTraditional Chinese Medicine Department, En Chu Kong Hospital, New Taipei City 40237, TaiwanBackground: Cardiomyopathy is one of the complications associated with diabetes. Due to its high prevalence, diabetic cardiomyopathy has become an urgent issue for diabetic patients. Various pathological signals are related to diabetic cardiomyopathy progress, including inflammasome. Mesenchymal stem cell transplantation is full of potential for the treatment of diabetic cardiomyopathy because of stem cell cardiac regenerative capability. This study investigates whether mesenchymal stem cell transplantation shows therapeutic effects on diabetic cardiomyopathy through inflammasome signaling regulation. Methods: Wistar male rats were divided into three groups including Sham, T1DM (rats with type 1 diabetes) and T1DM + WJSC (T1DM rats receiving 1 × 10<sup>6</sup> stem cells per rat). Results: Compared to the Sham, experimental results indicated that several pathological conditions can be observed in heart tissues with T1DM, including structural change, fibrosis, oxidative stress elevation and inflammasome related protein expression. All of these pathological conditions were significantly improved in T1DM rats receiving mesenchymal stem cell transplantation (T1DM + WJSC). Furthermore, the experimental findings suggest that mesenchymal stem cell transplantation exerted antioxidant protein expression in diabetic heart tissues, resulting in a decrease in oxidative stress and inflammasome signaling blockage. Conclusion: These findings imply that mesenchymal stem cell transplantation shows therapeutic effects on diabetic cardiomyopathy through inflammasome regulation induced by oxidative stress.https://www.mdpi.com/2308-3425/9/11/381cardiomyopathydiabetesinflammasomemesenchymal stem cellsreactive oxygen species |
| spellingShingle | Wei-Syun Hu Tung-Sheng Chen Ka-Hung Cheang Wei-Yu Liao Chin-Hsien Chang Mesenchymal Stem Cell Transplantation Increases Antioxidant Protein Expression and Ameliorates GP91/ROS/Inflammasome Signals in Diabetic Cardiomyopathy Journal of Cardiovascular Development and Disease cardiomyopathy diabetes inflammasome mesenchymal stem cells reactive oxygen species |
| title | Mesenchymal Stem Cell Transplantation Increases Antioxidant Protein Expression and Ameliorates GP91/ROS/Inflammasome Signals in Diabetic Cardiomyopathy |
| title_full | Mesenchymal Stem Cell Transplantation Increases Antioxidant Protein Expression and Ameliorates GP91/ROS/Inflammasome Signals in Diabetic Cardiomyopathy |
| title_fullStr | Mesenchymal Stem Cell Transplantation Increases Antioxidant Protein Expression and Ameliorates GP91/ROS/Inflammasome Signals in Diabetic Cardiomyopathy |
| title_full_unstemmed | Mesenchymal Stem Cell Transplantation Increases Antioxidant Protein Expression and Ameliorates GP91/ROS/Inflammasome Signals in Diabetic Cardiomyopathy |
| title_short | Mesenchymal Stem Cell Transplantation Increases Antioxidant Protein Expression and Ameliorates GP91/ROS/Inflammasome Signals in Diabetic Cardiomyopathy |
| title_sort | mesenchymal stem cell transplantation increases antioxidant protein expression and ameliorates gp91 ros inflammasome signals in diabetic cardiomyopathy |
| topic | cardiomyopathy diabetes inflammasome mesenchymal stem cells reactive oxygen species |
| url | https://www.mdpi.com/2308-3425/9/11/381 |
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