Infection-induced molecular pattern recognition proteins in larvae of Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae)
We cloned and sequenced full-length peptidoglycan recognition protein (PGRP)-like cDNAs, named PS PGRP-SA(a)-like, PS PGRP-SA(b)-like, PS PGRP-SB1-like and PS PGRP-SC-like, from Protaetia brevitarsis seulensis. The amino acid sequences of PS PGRPs share 32.03-47.93% homology with those of PGRP famil...
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| Format: | Article |
| Language: | English |
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Institute of Entomology, Biology Centre, Czech Academy of Science
2022-01-01
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| Series: | European Journal of Entomology |
| Subjects: | |
| Online Access: | https://www.eje.cz/artkey/eje-202201-0008_infection-induced_molecular_pattern_recognition_proteins_in_larvae_of_protaetia_brevitarsis_seulensis_coleopte.php |
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| author | Kyeongrin BANG Jiae LEE Sejung HWANG Youngwoo CHO Jangwoo PARK Saeyoull CHO |
| author_facet | Kyeongrin BANG Jiae LEE Sejung HWANG Youngwoo CHO Jangwoo PARK Saeyoull CHO |
| author_sort | Kyeongrin BANG |
| collection | DOAJ |
| description | We cloned and sequenced full-length peptidoglycan recognition protein (PGRP)-like cDNAs, named PS PGRP-SA(a)-like, PS PGRP-SA(b)-like, PS PGRP-SB1-like and PS PGRP-SC-like, from Protaetia brevitarsis seulensis. The amino acid sequences of PS PGRPs share 32.03-47.93% homology with those of PGRP family members in insects and mammals, including humans. We identified a conserved consensus sequence for amidase activity (His; H-Tyr; Y-His; H-Thr; T-Cys; C) and residues for binding peptidoglycan (PGN), one of the major bacterial cell wall components, including Asp (D) and Phe (F) for Lys-type PGN; and Gly(G), Trp (W) and Arg (R) for DAP-type PGN. The topological structures of PS PGRP-SA(a)-like, PS PGRP-SA(b)-like and PS PGRP-SC-like proteins are structurally similar to those of Drosophila melanogaster PGRP-SA, which has three α-helices and six β-strands. The β-strands are located in a central region and helix α1 on the back and peripheral α2 and α3 helices are on the front. The three α-helices and six β-strands are also present in PS PGRP-SB1-like, but the topological structure differs from that of typical PGRP. Significantly increased levels of PS PGRP-SA (a)-like and PS PGRP-SA (b)-like mRNA were recorded when Gram-positive bacteria or yeast cells were injected into larvae. PS PGRP-SB1-like mRNA levels were up-regulated by infection by all three pathogens; however, expression of PS PGRP-SC-like mRNA was increased 20- or 30-fold only shortly after injection with Gram-negative bacteria. |
| first_indexed | 2024-04-10T16:20:05Z |
| format | Article |
| id | doaj.art-42c53b138ad04586a0826715bcb32abe |
| institution | Directory Open Access Journal |
| issn | 1210-5759 1802-8829 |
| language | English |
| last_indexed | 2024-04-10T16:20:05Z |
| publishDate | 2022-01-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
| record_format | Article |
| series | European Journal of Entomology |
| spelling | doaj.art-42c53b138ad04586a0826715bcb32abe2023-02-09T13:52:51ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292022-01-011191778410.14411/eje.2022.008eje-202201-0008Infection-induced molecular pattern recognition proteins in larvae of Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae)Kyeongrin BANG0Jiae LEE1Sejung HWANG2Youngwoo CHO3Jangwoo PARK4Saeyoull CHO5Department of Applied Biology, College of Agriculture and Life Science, Kangwon National University, Chuncheon, Kangwon-do 200-701, South Korea; e-mails: bb1980@korea.kr, zloh1211@korea.kr, tpwjd321@korea.kr, 5120057@kangwon.ac.kr, saeyoullcho@kangwon.ac.krDepartment of Applied Biology, College of Agriculture and Life Science, Kangwon National University, Chuncheon, Kangwon-do 200-701, South Korea; e-mails: bb1980@korea.kr, zloh1211@korea.kr, tpwjd321@korea.kr, 5120057@kangwon.ac.kr, saeyoullcho@kangwon.ac.krDepartment of Applied Biology, College of Agriculture and Life Science, Kangwon National University, Chuncheon, Kangwon-do 200-701, South Korea; e-mails: bb1980@korea.kr, zloh1211@korea.kr, tpwjd321@korea.kr, 5120057@kangwon.ac.kr, saeyoullcho@kangwon.ac.krDepartment of Applied Biology, College of Agriculture and Life Science, Kangwon National University, Chuncheon, Kangwon-do 200-701, South Korea; e-mails: bb1980@korea.kr, zloh1211@korea.kr, tpwjd321@korea.kr, 5120057@kangwon.ac.kr, saeyoullcho@kangwon.ac.krOsang Kinsect Co., Guri, South Korea; e-mail: pjw@k-insect.comDepartment of Applied Biology, College of Agriculture and Life Science, Kangwon National University, Chuncheon, Kangwon-do 200-701, South Korea; e-mails: bb1980@korea.kr, zloh1211@korea.kr, tpwjd321@korea.kr, 5120057@kangwon.ac.kr, saeyoullcho@kangwon.ac.krWe cloned and sequenced full-length peptidoglycan recognition protein (PGRP)-like cDNAs, named PS PGRP-SA(a)-like, PS PGRP-SA(b)-like, PS PGRP-SB1-like and PS PGRP-SC-like, from Protaetia brevitarsis seulensis. The amino acid sequences of PS PGRPs share 32.03-47.93% homology with those of PGRP family members in insects and mammals, including humans. We identified a conserved consensus sequence for amidase activity (His; H-Tyr; Y-His; H-Thr; T-Cys; C) and residues for binding peptidoglycan (PGN), one of the major bacterial cell wall components, including Asp (D) and Phe (F) for Lys-type PGN; and Gly(G), Trp (W) and Arg (R) for DAP-type PGN. The topological structures of PS PGRP-SA(a)-like, PS PGRP-SA(b)-like and PS PGRP-SC-like proteins are structurally similar to those of Drosophila melanogaster PGRP-SA, which has three α-helices and six β-strands. The β-strands are located in a central region and helix α1 on the back and peripheral α2 and α3 helices are on the front. The three α-helices and six β-strands are also present in PS PGRP-SB1-like, but the topological structure differs from that of typical PGRP. Significantly increased levels of PS PGRP-SA (a)-like and PS PGRP-SA (b)-like mRNA were recorded when Gram-positive bacteria or yeast cells were injected into larvae. PS PGRP-SB1-like mRNA levels were up-regulated by infection by all three pathogens; however, expression of PS PGRP-SC-like mRNA was increased 20- or 30-fold only shortly after injection with Gram-negative bacteria.https://www.eje.cz/artkey/eje-202201-0008_infection-induced_molecular_pattern_recognition_proteins_in_larvae_of_protaetia_brevitarsis_seulensis_coleopte.phppgrpsinfectiongene expressionimmunity |
| spellingShingle | Kyeongrin BANG Jiae LEE Sejung HWANG Youngwoo CHO Jangwoo PARK Saeyoull CHO Infection-induced molecular pattern recognition proteins in larvae of Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) European Journal of Entomology pgrps infection gene expression immunity |
| title | Infection-induced molecular pattern recognition proteins in larvae of Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) |
| title_full | Infection-induced molecular pattern recognition proteins in larvae of Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) |
| title_fullStr | Infection-induced molecular pattern recognition proteins in larvae of Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) |
| title_full_unstemmed | Infection-induced molecular pattern recognition proteins in larvae of Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) |
| title_short | Infection-induced molecular pattern recognition proteins in larvae of Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) |
| title_sort | infection induced molecular pattern recognition proteins in larvae of protaetia brevitarsis seulensis coleoptera cetoniidae |
| topic | pgrps infection gene expression immunity |
| url | https://www.eje.cz/artkey/eje-202201-0008_infection-induced_molecular_pattern_recognition_proteins_in_larvae_of_protaetia_brevitarsis_seulensis_coleopte.php |
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