Arbuscular Mycorrhizal Community in Roots and Nitrogen Uptake Patterns of Understory Trees Beneath Ectomycorrhizal and Non-ectomycorrhizal Overstory Trees
Nitrogen (N) is an essential plant nutrient, and plants can take up N from several sources, including via mycorrhizal fungal associations. The N uptake patterns of understory plants may vary beneath different types of overstory trees, especially through the difference in their type of mycorrhizal as...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2020.583585/full |
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| author | Chikae Tatsumi Chikae Tatsumi Chikae Tatsumi Fujio Hyodo Takeshi Taniguchi Weiyu Shi Keisuke Koba Keitaro Fukushima Sheng Du Norikazu Yamanaka Pamela Templer Ryunosuke Tateno |
| author_facet | Chikae Tatsumi Chikae Tatsumi Chikae Tatsumi Fujio Hyodo Takeshi Taniguchi Weiyu Shi Keisuke Koba Keitaro Fukushima Sheng Du Norikazu Yamanaka Pamela Templer Ryunosuke Tateno |
| author_sort | Chikae Tatsumi |
| collection | DOAJ |
| description | Nitrogen (N) is an essential plant nutrient, and plants can take up N from several sources, including via mycorrhizal fungal associations. The N uptake patterns of understory plants may vary beneath different types of overstory trees, especially through the difference in their type of mycorrhizal association (arbuscular mycorrhizal, AM; or ectomycorrhizal, ECM), because soil mycorrhizal community and N availability differ beneath AM (non-ECM) and ECM overstory trees (e.g., relatively low nitrate content beneath ECM overstory trees). To test this hypothesis, we examined six co-existing AM-symbiotic understory tree species common beneath both AM-symbiotic black locust (non-ECM) and ECM-symbiotic oak trees of dryland forests in China. We measured AM fungal community composition of roots and natural abundance stable isotopic composition of N (δ15N) in plant leaves, roots, and soils. The root mycorrhizal community composition of understory trees did not significantly differ between beneath non-ECM and ECM overstory trees, although some OTUs more frequently appeared beneath non-ECM trees. Understory trees beneath non-ECM overstory trees had similar δ15N values in leaves and soil nitrate, suggesting that they took up most of their nitrogen as nitrate. Beneath ECM overstory trees, understory trees had consistently lower leaf than root δ15N, suggesting they depended on mycorrhizal fungi for N acquisition since mycorrhizal fungi transfer isotopically light N to host plants. Additionally, leaf N concentrations in the understory trees were lower beneath ECM than the non-ECM overstory trees. Our results show that, without large differences in root mycorrhizal community, the N uptake patterns of understory trees vary between beneath different overstory trees. |
| first_indexed | 2024-12-17T21:18:18Z |
| format | Article |
| id | doaj.art-42c626a2dc3b46af8a7b7ff5c0773539 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-17T21:18:18Z |
| publishDate | 2021-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-42c626a2dc3b46af8a7b7ff5c07735392022-12-21T21:32:16ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-01-011110.3389/fpls.2020.583585583585Arbuscular Mycorrhizal Community in Roots and Nitrogen Uptake Patterns of Understory Trees Beneath Ectomycorrhizal and Non-ectomycorrhizal Overstory TreesChikae Tatsumi0Chikae Tatsumi1Chikae Tatsumi2Fujio Hyodo3Takeshi Taniguchi4Weiyu Shi5Keisuke Koba6Keitaro Fukushima7Sheng Du8Norikazu Yamanaka9Pamela Templer10Ryunosuke Tateno11Research Faculty of Agriculture, Hokkaido University, Sapporo, JapanGraduate School of Agriculture, Kyoto University, Kyoto, JapanDepartment of Biology, Boston University, Boston, MA, United StatesResearch Core for Interdisciplinary Sciences, Okayama University, Okayama, JapanArid Land Research Center, Tottori University, Tottori, JapanSchool of Geographical Sciences, Southwest University, Chongqing, ChinaCenter for Ecological Research, Kyoto University, Shiga, JapanCenter for Ecological Research, Kyoto University, Shiga, JapanState Key Laboratory of Soil Erosion and Dryland Farming on Loess Plateau, Institute of Soil and Water Conservation, Chinese Academy of Sciences, Beijing, ChinaArid Land Research Center, Tottori University, Tottori, JapanDepartment of Biology, Boston University, Boston, MA, United StatesField Science Education and Research Center, Kyoto University, Kyoto, JapanNitrogen (N) is an essential plant nutrient, and plants can take up N from several sources, including via mycorrhizal fungal associations. The N uptake patterns of understory plants may vary beneath different types of overstory trees, especially through the difference in their type of mycorrhizal association (arbuscular mycorrhizal, AM; or ectomycorrhizal, ECM), because soil mycorrhizal community and N availability differ beneath AM (non-ECM) and ECM overstory trees (e.g., relatively low nitrate content beneath ECM overstory trees). To test this hypothesis, we examined six co-existing AM-symbiotic understory tree species common beneath both AM-symbiotic black locust (non-ECM) and ECM-symbiotic oak trees of dryland forests in China. We measured AM fungal community composition of roots and natural abundance stable isotopic composition of N (δ15N) in plant leaves, roots, and soils. The root mycorrhizal community composition of understory trees did not significantly differ between beneath non-ECM and ECM overstory trees, although some OTUs more frequently appeared beneath non-ECM trees. Understory trees beneath non-ECM overstory trees had similar δ15N values in leaves and soil nitrate, suggesting that they took up most of their nitrogen as nitrate. Beneath ECM overstory trees, understory trees had consistently lower leaf than root δ15N, suggesting they depended on mycorrhizal fungi for N acquisition since mycorrhizal fungi transfer isotopically light N to host plants. Additionally, leaf N concentrations in the understory trees were lower beneath ECM than the non-ECM overstory trees. Our results show that, without large differences in root mycorrhizal community, the N uptake patterns of understory trees vary between beneath different overstory trees.https://www.frontiersin.org/articles/10.3389/fpls.2020.583585/full15N natural abundancearbuscular mycorrhizal fungidrylandectomycorrhizal fungi (ECM fungi)mycorrhizal dependencenitrate |
| spellingShingle | Chikae Tatsumi Chikae Tatsumi Chikae Tatsumi Fujio Hyodo Takeshi Taniguchi Weiyu Shi Keisuke Koba Keitaro Fukushima Sheng Du Norikazu Yamanaka Pamela Templer Ryunosuke Tateno Arbuscular Mycorrhizal Community in Roots and Nitrogen Uptake Patterns of Understory Trees Beneath Ectomycorrhizal and Non-ectomycorrhizal Overstory Trees Frontiers in Plant Science 15N natural abundance arbuscular mycorrhizal fungi dryland ectomycorrhizal fungi (ECM fungi) mycorrhizal dependence nitrate |
| title | Arbuscular Mycorrhizal Community in Roots and Nitrogen Uptake Patterns of Understory Trees Beneath Ectomycorrhizal and Non-ectomycorrhizal Overstory Trees |
| title_full | Arbuscular Mycorrhizal Community in Roots and Nitrogen Uptake Patterns of Understory Trees Beneath Ectomycorrhizal and Non-ectomycorrhizal Overstory Trees |
| title_fullStr | Arbuscular Mycorrhizal Community in Roots and Nitrogen Uptake Patterns of Understory Trees Beneath Ectomycorrhizal and Non-ectomycorrhizal Overstory Trees |
| title_full_unstemmed | Arbuscular Mycorrhizal Community in Roots and Nitrogen Uptake Patterns of Understory Trees Beneath Ectomycorrhizal and Non-ectomycorrhizal Overstory Trees |
| title_short | Arbuscular Mycorrhizal Community in Roots and Nitrogen Uptake Patterns of Understory Trees Beneath Ectomycorrhizal and Non-ectomycorrhizal Overstory Trees |
| title_sort | arbuscular mycorrhizal community in roots and nitrogen uptake patterns of understory trees beneath ectomycorrhizal and non ectomycorrhizal overstory trees |
| topic | 15N natural abundance arbuscular mycorrhizal fungi dryland ectomycorrhizal fungi (ECM fungi) mycorrhizal dependence nitrate |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2020.583585/full |
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