Piezoresistive microcantilever aptasensor for ricin detection and kinetic analysis
Up to now, there has been no report on target molecules detection by a piezoresistive microcantilever aptasensor. In order to evaluate the test performance and investigate the response dynamic characteristics of a piezoresistive microcantilever aptasensor, a novel method for ricin detection and kine...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
AIP Publishing LLC
2015-04-01
|
| Series: | AIP Advances |
| Online Access: | http://dx.doi.org/10.1063/1.4907996 |
| _version_ | 1818333922021343232 |
|---|---|
| author | Zhi-Wei Liu Zhao-Yang Tong Bing Liu Lan-Qun Hao Xi-Hui Mu Jin-Ping Zhang Chuan Gao |
| author_facet | Zhi-Wei Liu Zhao-Yang Tong Bing Liu Lan-Qun Hao Xi-Hui Mu Jin-Ping Zhang Chuan Gao |
| author_sort | Zhi-Wei Liu |
| collection | DOAJ |
| description | Up to now, there has been no report on target molecules detection by a piezoresistive microcantilever aptasensor. In order to evaluate the test performance and investigate the response dynamic characteristics of a piezoresistive microcantilever aptasensor, a novel method for ricin detection and kinetic analysis based on a piezoresistive microcantilever aptasensor was proposed, where ricin aptamer was immobilised on the microcantilever surface by biotin-avidin binding system. Results showed that the detection limit of ricin was 0.04μg L−1 (S/N ≥ 3). A linear relationship between the response voltage and the concentration of ricin in the range of 0.2μg L−1-40μg L−1 was obtained, with the linear regression equation of ΔUe = 0.904C + 5.852 (n = 5, R = 0.991, p < 0.001). The sensor showed no response for abrin, BSA, and could overcome the influence of complex environmental disruptors, indicating high specificity and good selectivity. Recovery and reproducibility in the result of simulated samples (simulated water, soil, and flour sample) determination met the analysis requirements, which was 90.5∼95.5% and 7.85%∼9.39%, respectively. On this basis, a reaction kinetic model based on ligand-receptor binding and the relationship with response voltage was established. The model could well reflect the dynamic response of the sensor. The correlation coefficient (R) was greater than or equal to 0.9456 (p < 0.001). Response voltage (ΔUe) and response time (t0) obtained from the fitting equation on different concentrations of ricin fitted well with the measured values. |
| first_indexed | 2024-12-13T13:59:20Z |
| format | Article |
| id | doaj.art-42c67bf8de504c50b0a6e19a572a2da0 |
| institution | Directory Open Access Journal |
| issn | 2158-3226 |
| language | English |
| last_indexed | 2024-12-13T13:59:20Z |
| publishDate | 2015-04-01 |
| publisher | AIP Publishing LLC |
| record_format | Article |
| series | AIP Advances |
| spelling | doaj.art-42c67bf8de504c50b0a6e19a572a2da02022-12-21T23:42:47ZengAIP Publishing LLCAIP Advances2158-32262015-04-0154041324041324-810.1063/1.4907996022593ADVPiezoresistive microcantilever aptasensor for ricin detection and kinetic analysisZhi-Wei Liu0Zhao-Yang Tong1Bing Liu2Lan-Qun Hao3Xi-Hui Mu4Jin-Ping Zhang5Chuan Gao6State Key Laboratory of NBC Protection for Civilians, Research Institute of Chemical Defence, Beijing 102205, ChinaState Key Laboratory of NBC Protection for Civilians, Research Institute of Chemical Defence, Beijing 102205, ChinaState Key Laboratory of NBC Protection for Civilians, Research Institute of Chemical Defence, Beijing 102205, ChinaState Key Laboratory of NBC Protection for Civilians, Research Institute of Chemical Defence, Beijing 102205, ChinaState Key Laboratory of NBC Protection for Civilians, Research Institute of Chemical Defence, Beijing 102205, ChinaState Key Laboratory of NBC Protection for Civilians, Research Institute of Chemical Defence, Beijing 102205, ChinaState Key Laboratory of NBC Protection for Civilians, Research Institute of Chemical Defence, Beijing 102205, ChinaUp to now, there has been no report on target molecules detection by a piezoresistive microcantilever aptasensor. In order to evaluate the test performance and investigate the response dynamic characteristics of a piezoresistive microcantilever aptasensor, a novel method for ricin detection and kinetic analysis based on a piezoresistive microcantilever aptasensor was proposed, where ricin aptamer was immobilised on the microcantilever surface by biotin-avidin binding system. Results showed that the detection limit of ricin was 0.04μg L−1 (S/N ≥ 3). A linear relationship between the response voltage and the concentration of ricin in the range of 0.2μg L−1-40μg L−1 was obtained, with the linear regression equation of ΔUe = 0.904C + 5.852 (n = 5, R = 0.991, p < 0.001). The sensor showed no response for abrin, BSA, and could overcome the influence of complex environmental disruptors, indicating high specificity and good selectivity. Recovery and reproducibility in the result of simulated samples (simulated water, soil, and flour sample) determination met the analysis requirements, which was 90.5∼95.5% and 7.85%∼9.39%, respectively. On this basis, a reaction kinetic model based on ligand-receptor binding and the relationship with response voltage was established. The model could well reflect the dynamic response of the sensor. The correlation coefficient (R) was greater than or equal to 0.9456 (p < 0.001). Response voltage (ΔUe) and response time (t0) obtained from the fitting equation on different concentrations of ricin fitted well with the measured values.http://dx.doi.org/10.1063/1.4907996 |
| spellingShingle | Zhi-Wei Liu Zhao-Yang Tong Bing Liu Lan-Qun Hao Xi-Hui Mu Jin-Ping Zhang Chuan Gao Piezoresistive microcantilever aptasensor for ricin detection and kinetic analysis AIP Advances |
| title | Piezoresistive microcantilever aptasensor for ricin detection and kinetic analysis |
| title_full | Piezoresistive microcantilever aptasensor for ricin detection and kinetic analysis |
| title_fullStr | Piezoresistive microcantilever aptasensor for ricin detection and kinetic analysis |
| title_full_unstemmed | Piezoresistive microcantilever aptasensor for ricin detection and kinetic analysis |
| title_short | Piezoresistive microcantilever aptasensor for ricin detection and kinetic analysis |
| title_sort | piezoresistive microcantilever aptasensor for ricin detection and kinetic analysis |
| url | http://dx.doi.org/10.1063/1.4907996 |
| work_keys_str_mv | AT zhiweiliu piezoresistivemicrocantileveraptasensorforricindetectionandkineticanalysis AT zhaoyangtong piezoresistivemicrocantileveraptasensorforricindetectionandkineticanalysis AT bingliu piezoresistivemicrocantileveraptasensorforricindetectionandkineticanalysis AT lanqunhao piezoresistivemicrocantileveraptasensorforricindetectionandkineticanalysis AT xihuimu piezoresistivemicrocantileveraptasensorforricindetectionandkineticanalysis AT jinpingzhang piezoresistivemicrocantileveraptasensorforricindetectionandkineticanalysis AT chuangao piezoresistivemicrocantileveraptasensorforricindetectionandkineticanalysis |