Effect of Conjugated Blackcurrant Extract and Zinc Oxide Nanoparticles on Histopathologic Damage of Kidney Tissue in Diabetic Rats
Background & objectives: One of the most important diabetes complications is chronic renal failure. This disorder is one of the most important progressive renal problems that is closely associated with high mortality rate in diabetic patients. The purpose of this study was to evaluate the effect...
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| Format: | Article |
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Ardabil University of Medical Sciences
2019-03-01
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| Series: | Journal of Ardabil University of Medical Sciences |
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| Online Access: | http://jarums.arums.ac.ir/browse.php?a_code=A-10-1073-2&slc_lang=en&sid=1 |
| _version_ | 1818242378989830144 |
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| author | Sepideh Ahmadi Sagerlou Reza Alipanah-Moghadam Ramin Salimnejad |
| author_facet | Sepideh Ahmadi Sagerlou Reza Alipanah-Moghadam Ramin Salimnejad |
| author_sort | Sepideh Ahmadi Sagerlou |
| collection | DOAJ |
| description | Background & objectives: One of the most important diabetes complications is chronic renal failure. This disorder is one of the most important progressive renal problems that is closely associated with high mortality rate in diabetic patients. The purpose of this study was to evaluate the effect of conjugated blackcurrant extract and zinc oxide nanoparticles on histopathologic damages of kidney tissue in diabetic rats.
Methods: A total of thirty adult Wistar rats were used and they were randomly divided into the following six groups (n=5): control group; diabetic group (170 mg/kg alloxan); diabetic group treated with insulin (170 mg/kg alloxan & 10 U/kg insulin); diabetic group treated with ZnO nanoparticle (170 mg/kg alloxan & 8 mg/kg ZnO nanoparticle); diabetic group treated with extract (170 mg/kg alloxan & 150 mg/kg blackcurrant extract) and diabetic group treated with extract conjugated with ZnO nanoparticles (170 mg/kg alloxan & 8 mg/kg blackcurrant extract conjugated with ZnO nanoparticle). Diabetes mellitus was induced by intraperitoneal injection of alloxan monohydrate. Conjugated blackcurrant extract and ZnO nanoparticles were produced using green synthesis method. The tissues were stained with hematoxylin and eosin stain. Image J software was used for evaluation of histopathological changes.
Results: The comparison between groups treated with blackcurrant extract, ZnO nanoparticles and blackcurrant extract conjugated with ZnO nanoparticle showed that these compounds somewhat protect kidney tissue from diabetes damage . The highest protective effect was observed in diabetic group treated with ZnO nanoparticles conjugated with blackcurrant extract.
Conclusion: Our study, for the first time, showed that the conjugation of blackcurrant extract to ZnO nanoparticles can be effective in preventing renal- tissue damage in diabetic patient. |
| first_indexed | 2024-12-12T13:44:17Z |
| format | Article |
| id | doaj.art-42c9756bbbe74448966f7cac72eda09c |
| institution | Directory Open Access Journal |
| issn | 2228-7280 2228-7299 |
| language | fas |
| last_indexed | 2024-12-12T13:44:17Z |
| publishDate | 2019-03-01 |
| publisher | Ardabil University of Medical Sciences |
| record_format | Article |
| series | Journal of Ardabil University of Medical Sciences |
| spelling | doaj.art-42c9756bbbe74448966f7cac72eda09c2022-12-22T00:22:43ZfasArdabil University of Medical SciencesJournal of Ardabil University of Medical Sciences2228-72802228-72992019-03-01184419428Effect of Conjugated Blackcurrant Extract and Zinc Oxide Nanoparticles on Histopathologic Damage of Kidney Tissue in Diabetic RatsSepideh Ahmadi Sagerlou0Reza Alipanah-Moghadam1Ramin Salimnejad2 Department of Clinical Biochemistry, School of Medicine, Ardabil University of Medical Sciences , Ardabil, Iran Department of Clinical Biochemistry, School of Medicine, Ardabil University of Medical Sciences , Ardabil, Iran Background & objectives: One of the most important diabetes complications is chronic renal failure. This disorder is one of the most important progressive renal problems that is closely associated with high mortality rate in diabetic patients. The purpose of this study was to evaluate the effect of conjugated blackcurrant extract and zinc oxide nanoparticles on histopathologic damages of kidney tissue in diabetic rats. Methods: A total of thirty adult Wistar rats were used and they were randomly divided into the following six groups (n=5): control group; diabetic group (170 mg/kg alloxan); diabetic group treated with insulin (170 mg/kg alloxan & 10 U/kg insulin); diabetic group treated with ZnO nanoparticle (170 mg/kg alloxan & 8 mg/kg ZnO nanoparticle); diabetic group treated with extract (170 mg/kg alloxan & 150 mg/kg blackcurrant extract) and diabetic group treated with extract conjugated with ZnO nanoparticles (170 mg/kg alloxan & 8 mg/kg blackcurrant extract conjugated with ZnO nanoparticle). Diabetes mellitus was induced by intraperitoneal injection of alloxan monohydrate. Conjugated blackcurrant extract and ZnO nanoparticles were produced using green synthesis method. The tissues were stained with hematoxylin and eosin stain. Image J software was used for evaluation of histopathological changes. Results: The comparison between groups treated with blackcurrant extract, ZnO nanoparticles and blackcurrant extract conjugated with ZnO nanoparticle showed that these compounds somewhat protect kidney tissue from diabetes damage . The highest protective effect was observed in diabetic group treated with ZnO nanoparticles conjugated with blackcurrant extract. Conclusion: Our study, for the first time, showed that the conjugation of blackcurrant extract to ZnO nanoparticles can be effective in preventing renal- tissue damage in diabetic patient.http://jarums.arums.ac.ir/browse.php?a_code=A-10-1073-2&slc_lang=en&sid=1BlackcurrantZnO NanoparticlesDiabetesRenal Damage |
| spellingShingle | Sepideh Ahmadi Sagerlou Reza Alipanah-Moghadam Ramin Salimnejad Effect of Conjugated Blackcurrant Extract and Zinc Oxide Nanoparticles on Histopathologic Damage of Kidney Tissue in Diabetic Rats Journal of Ardabil University of Medical Sciences Blackcurrant ZnO Nanoparticles Diabetes Renal Damage |
| title | Effect of Conjugated Blackcurrant Extract and Zinc Oxide Nanoparticles on Histopathologic Damage of Kidney Tissue in Diabetic Rats |
| title_full | Effect of Conjugated Blackcurrant Extract and Zinc Oxide Nanoparticles on Histopathologic Damage of Kidney Tissue in Diabetic Rats |
| title_fullStr | Effect of Conjugated Blackcurrant Extract and Zinc Oxide Nanoparticles on Histopathologic Damage of Kidney Tissue in Diabetic Rats |
| title_full_unstemmed | Effect of Conjugated Blackcurrant Extract and Zinc Oxide Nanoparticles on Histopathologic Damage of Kidney Tissue in Diabetic Rats |
| title_short | Effect of Conjugated Blackcurrant Extract and Zinc Oxide Nanoparticles on Histopathologic Damage of Kidney Tissue in Diabetic Rats |
| title_sort | effect of conjugated blackcurrant extract and zinc oxide nanoparticles on histopathologic damage of kidney tissue in diabetic rats |
| topic | Blackcurrant ZnO Nanoparticles Diabetes Renal Damage |
| url | http://jarums.arums.ac.ir/browse.php?a_code=A-10-1073-2&slc_lang=en&sid=1 |
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