Development of an Electrochemical Immunosensor for Specific Detection of Visceral Leishmaniasis Using Gold-Modified Screen-Printed Carbon Electrodes
Visceral leishmaniasis is a reemerging neglected tropical disease with limitations for its diagnosis, including low concentration of antibodies in the serum of asymptomatic patients and cross-reactions. In this context, this work proposes an electrochemical immunosensor for the diagnosis of visceral...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/10/8/81 |
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| author | Beatriz R. Martins Yanne O. Barbosa Cristhianne M. R. Andrade Loren Q. Pereira Guilherme F. Simão Carlo J. de Oliveira Dalmo Correia Robson T. S. Oliveira Marcos V. da Silva Anielle C. A. Silva Noelio O. Dantas Virmondes Rodrigues Rodrigo A. A. Muñoz Renata P. Alves-Balvedi |
| author_facet | Beatriz R. Martins Yanne O. Barbosa Cristhianne M. R. Andrade Loren Q. Pereira Guilherme F. Simão Carlo J. de Oliveira Dalmo Correia Robson T. S. Oliveira Marcos V. da Silva Anielle C. A. Silva Noelio O. Dantas Virmondes Rodrigues Rodrigo A. A. Muñoz Renata P. Alves-Balvedi |
| author_sort | Beatriz R. Martins |
| collection | DOAJ |
| description | Visceral leishmaniasis is a reemerging neglected tropical disease with limitations for its diagnosis, including low concentration of antibodies in the serum of asymptomatic patients and cross-reactions. In this context, this work proposes an electrochemical immunosensor for the diagnosis of visceral leishmaniasis in a more sensitive way that is capable of avoiding cross-reaction with Chagas disease (CD). Crude <i>Leishmania infantum</i> antigens tested in the enzyme-linked immunosorbent assay (ELISA) were methodologically standardized to best engage to the sensor. The antibodies anti-<i>Trypanosoma cruzi</i> and anti-<i>Leishmania</i> sp. Present in serum from patients with diverse types of CD or leishmaniasis were chosen. A screen-printed carbon electrode modified with gold nanoparticles was the best platform to guarantee effective adsorption of all antigens so that the epitope of specific recognition for leishmaniasis occurred efficiently and without cross-reaction with the evaluated CD. The current peaks reduced linearly after the recognition, and still were able to notice the discrimination between different kinds of diseases (digestive, cardiac, undetermined Chagas/acute and visceral chronic leishmaniasis). Comparative analyses with ELISA were performed with the same groups, and a low specificity (44%) was verified due to cross-reactions (high number of false positives) on ELISA tests, while the proposed immunosensor presented high selectivity and specificity (100%) without any false positives or false negatives for the serum samples from isolated patients with different types of CD and visceral leishmaniasis. Furthermore, the biosensor was stable for 5 days and presented a detection limit of 200 ng mL<sup>−1</sup>. |
| first_indexed | 2024-03-10T18:16:47Z |
| format | Article |
| id | doaj.art-42ccc62eb1a64489aa90881371e9ac11 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T18:16:47Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-42ccc62eb1a64489aa90881371e9ac112023-11-20T07:39:12ZengMDPI AGBiosensors2079-63742020-07-011088110.3390/bios10080081Development of an Electrochemical Immunosensor for Specific Detection of Visceral Leishmaniasis Using Gold-Modified Screen-Printed Carbon ElectrodesBeatriz R. Martins0Yanne O. Barbosa1Cristhianne M. R. Andrade2Loren Q. Pereira3Guilherme F. Simão4Carlo J. de Oliveira5Dalmo Correia6Robson T. S. Oliveira7Marcos V. da Silva8Anielle C. A. Silva9Noelio O. Dantas10Virmondes Rodrigues11Rodrigo A. A. Muñoz12Renata P. Alves-Balvedi13Institute of Biological and Natural Sciences, Federal University of Triângulo Mineiro, Uberaba-MG 38025-180, BrazilInstitute of Biological and Natural Sciences, Federal University of Triângulo Mineiro, Uberaba-MG 38025-180, BrazilInstitute of Health Sciences, Federal University of Triângulo Mineiro, Uberaba-MG 38025-180, BrazilInstitute of Health Sciences, Federal University of Triângulo Mineiro, Uberaba-MG 38025-180, BrazilInstitute of Technological and Exact Sciences, Federal University of Triângulo Mineiro, Uberaba-MG 38025-180, BrazilInstitute of Biological and Natural Sciences, Federal University of Triângulo Mineiro, Uberaba-MG 38025-180, BrazilInstitute of Health Sciences, Federal University of Triângulo Mineiro, Uberaba-MG 38025-180, BrazilInstitute of Biological and Natural Sciences, Federal University of Triângulo Mineiro, Uberaba-MG 38025-180, BrazilInstitute of Health Sciences, Federal University of Triângulo Mineiro, Uberaba-MG 38025-180, BrazilInstitute of Physics, Federal University of Alagoas, Maceio-AL 57072-970, BrazilInstitute of Physics, Federal University of Alagoas, Maceio-AL 57072-970, BrazilInstitute of Biological and Natural Sciences, Federal University of Triângulo Mineiro, Uberaba-MG 38025-180, BrazilInstitute of Chemistry, Federal University of Uberlândia, Uberlândia-MG 38408-100, BrazilInstitute of Biological and Natural Sciences, Federal University of Triângulo Mineiro, Uberaba-MG 38025-180, BrazilVisceral leishmaniasis is a reemerging neglected tropical disease with limitations for its diagnosis, including low concentration of antibodies in the serum of asymptomatic patients and cross-reactions. In this context, this work proposes an electrochemical immunosensor for the diagnosis of visceral leishmaniasis in a more sensitive way that is capable of avoiding cross-reaction with Chagas disease (CD). Crude <i>Leishmania infantum</i> antigens tested in the enzyme-linked immunosorbent assay (ELISA) were methodologically standardized to best engage to the sensor. The antibodies anti-<i>Trypanosoma cruzi</i> and anti-<i>Leishmania</i> sp. Present in serum from patients with diverse types of CD or leishmaniasis were chosen. A screen-printed carbon electrode modified with gold nanoparticles was the best platform to guarantee effective adsorption of all antigens so that the epitope of specific recognition for leishmaniasis occurred efficiently and without cross-reaction with the evaluated CD. The current peaks reduced linearly after the recognition, and still were able to notice the discrimination between different kinds of diseases (digestive, cardiac, undetermined Chagas/acute and visceral chronic leishmaniasis). Comparative analyses with ELISA were performed with the same groups, and a low specificity (44%) was verified due to cross-reactions (high number of false positives) on ELISA tests, while the proposed immunosensor presented high selectivity and specificity (100%) without any false positives or false negatives for the serum samples from isolated patients with different types of CD and visceral leishmaniasis. Furthermore, the biosensor was stable for 5 days and presented a detection limit of 200 ng mL<sup>−1</sup>.https://www.mdpi.com/2079-6374/10/8/81electrochemical biosensorvisceral leishmaniasisChagas diseasegold nanoparticlespoint-of-careportable analysis |
| spellingShingle | Beatriz R. Martins Yanne O. Barbosa Cristhianne M. R. Andrade Loren Q. Pereira Guilherme F. Simão Carlo J. de Oliveira Dalmo Correia Robson T. S. Oliveira Marcos V. da Silva Anielle C. A. Silva Noelio O. Dantas Virmondes Rodrigues Rodrigo A. A. Muñoz Renata P. Alves-Balvedi Development of an Electrochemical Immunosensor for Specific Detection of Visceral Leishmaniasis Using Gold-Modified Screen-Printed Carbon Electrodes Biosensors electrochemical biosensor visceral leishmaniasis Chagas disease gold nanoparticles point-of-care portable analysis |
| title | Development of an Electrochemical Immunosensor for Specific Detection of Visceral Leishmaniasis Using Gold-Modified Screen-Printed Carbon Electrodes |
| title_full | Development of an Electrochemical Immunosensor for Specific Detection of Visceral Leishmaniasis Using Gold-Modified Screen-Printed Carbon Electrodes |
| title_fullStr | Development of an Electrochemical Immunosensor for Specific Detection of Visceral Leishmaniasis Using Gold-Modified Screen-Printed Carbon Electrodes |
| title_full_unstemmed | Development of an Electrochemical Immunosensor for Specific Detection of Visceral Leishmaniasis Using Gold-Modified Screen-Printed Carbon Electrodes |
| title_short | Development of an Electrochemical Immunosensor for Specific Detection of Visceral Leishmaniasis Using Gold-Modified Screen-Printed Carbon Electrodes |
| title_sort | development of an electrochemical immunosensor for specific detection of visceral leishmaniasis using gold modified screen printed carbon electrodes |
| topic | electrochemical biosensor visceral leishmaniasis Chagas disease gold nanoparticles point-of-care portable analysis |
| url | https://www.mdpi.com/2079-6374/10/8/81 |
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