Optimisation of a Lawsonia inermis L. micropropagation protocol and acclimatization in a hydroponic culture

Lawsonia inermis is an important industrial and medicinal plant, cultivated mostly for dye production which is used in cosmetic industry. The objective of this study was establishing an efficient in vitro propagation system in order to obtain plants that will be acclimatized and grown hydro...

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Main Authors: Marković Marija, Đunisijević-Bojović Danijela, Milutinović Marijana, Petrović Jelena
Format: Article
Language:srp
Published: Faculty of Forestry, Belgrade 2020-01-01
Series:Glasnik Šumarskog Fakulteta: Univerzitet u Beogradu
Subjects:
Online Access:http://www.doiserbia.nb.rs/img/doi/0353-4537/2020/0353-45372021091M.pdf
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author Marković Marija
Đunisijević-Bojović Danijela
Milutinović Marijana
Petrović Jelena
author_facet Marković Marija
Đunisijević-Bojović Danijela
Milutinović Marijana
Petrović Jelena
author_sort Marković Marija
collection DOAJ
description Lawsonia inermis is an important industrial and medicinal plant, cultivated mostly for dye production which is used in cosmetic industry. The objective of this study was establishing an efficient in vitro propagation system in order to obtain plants that will be acclimatized and grown hydroponically. The effect of different media on in vitro rooting were examined, followed by growing obtained microplants in a hydroponic culture, in a half-strength modified Hoagland nutrient solution. The highest rooting rate (79.2%) was recorded on the half-strength MS media containing 0.50 mg.L-1 IBA, with a high average number of roots (7.4). Those microplants acclimatized well in a hydroponic culture, where very rapid growth was recorded, and well developed roots and shoots were formed. After transplanting plants from hydroponic to soil, the survival rate was 100%. This is the first study reporting an acclimatization procedure in a hydroponic for the henna plant.
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spelling doaj.art-42d508ad8cc242baa85f37576df150b02022-12-22T01:32:49ZsrpFaculty of Forestry, BelgradeGlasnik Šumarskog Fakulteta: Univerzitet u Beogradu0353-45372217-86002020-01-012020121919610.2298/GSF2021091M0353-45372021091MOptimisation of a Lawsonia inermis L. micropropagation protocol and acclimatization in a hydroponic cultureMarković Marija0Đunisijević-Bojović Danijela1Milutinović Marijana2Petrović Jelena3University of Belgrade, Faculty of Forestry, Belgrade, SerbiaUniversity of Belgrade, Faculty of Forestry, Belgrade, SerbiaUniversity of Belgrade, Faculty of Forestry, Belgrade, SerbiaUniversity of Belgrade, Faculty of Forestry, Belgrade, SerbiaLawsonia inermis is an important industrial and medicinal plant, cultivated mostly for dye production which is used in cosmetic industry. The objective of this study was establishing an efficient in vitro propagation system in order to obtain plants that will be acclimatized and grown hydroponically. The effect of different media on in vitro rooting were examined, followed by growing obtained microplants in a hydroponic culture, in a half-strength modified Hoagland nutrient solution. The highest rooting rate (79.2%) was recorded on the half-strength MS media containing 0.50 mg.L-1 IBA, with a high average number of roots (7.4). Those microplants acclimatized well in a hydroponic culture, where very rapid growth was recorded, and well developed roots and shoots were formed. After transplanting plants from hydroponic to soil, the survival rate was 100%. This is the first study reporting an acclimatization procedure in a hydroponic for the henna plant.http://www.doiserbia.nb.rs/img/doi/0353-4537/2020/0353-45372021091M.pdfacclimatizationhennahydroponicin vitro rootingplant growth regulators
spellingShingle Marković Marija
Đunisijević-Bojović Danijela
Milutinović Marijana
Petrović Jelena
Optimisation of a Lawsonia inermis L. micropropagation protocol and acclimatization in a hydroponic culture
Glasnik Šumarskog Fakulteta: Univerzitet u Beogradu
acclimatization
henna
hydroponic
in vitro rooting
plant growth regulators
title Optimisation of a Lawsonia inermis L. micropropagation protocol and acclimatization in a hydroponic culture
title_full Optimisation of a Lawsonia inermis L. micropropagation protocol and acclimatization in a hydroponic culture
title_fullStr Optimisation of a Lawsonia inermis L. micropropagation protocol and acclimatization in a hydroponic culture
title_full_unstemmed Optimisation of a Lawsonia inermis L. micropropagation protocol and acclimatization in a hydroponic culture
title_short Optimisation of a Lawsonia inermis L. micropropagation protocol and acclimatization in a hydroponic culture
title_sort optimisation of a lawsonia inermis l micropropagation protocol and acclimatization in a hydroponic culture
topic acclimatization
henna
hydroponic
in vitro rooting
plant growth regulators
url http://www.doiserbia.nb.rs/img/doi/0353-4537/2020/0353-45372021091M.pdf
work_keys_str_mv AT markovicmarija optimisationofalawsoniainermislmicropropagationprotocolandacclimatizationinahydroponicculture
AT đunisijevicbojovicdanijela optimisationofalawsoniainermislmicropropagationprotocolandacclimatizationinahydroponicculture
AT milutinovicmarijana optimisationofalawsoniainermislmicropropagationprotocolandacclimatizationinahydroponicculture
AT petrovicjelena optimisationofalawsoniainermislmicropropagationprotocolandacclimatizationinahydroponicculture