<i>Bixa orellana</i> L. (Bixaceae) and <i>Dysphania ambrosioides</i> (L.) Mosyakin & Clemants (Amaranthaceae) Essential Oils Formulated in Nanocochleates against <i>Leishmania amazonensis</i>
Leishmaniasis is a group of neglected tropical diseases caused by protozoan parasites of the <i>Leishmania</i> genus. The absence of effective vaccines and the limitations of current treatments make the search for effective therapies a real need. Different plant-derived essential oils (E...
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| Format: | Article |
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MDPI AG
2019-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/23/4222 |
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| author | Laura Machín Beatriz Tamargo Abel Piñón Regla C. Atíes Ramón Scull William N. Setzer Lianet Monzote |
| author_facet | Laura Machín Beatriz Tamargo Abel Piñón Regla C. Atíes Ramón Scull William N. Setzer Lianet Monzote |
| author_sort | Laura Machín |
| collection | DOAJ |
| description | Leishmaniasis is a group of neglected tropical diseases caused by protozoan parasites of the <i>Leishmania</i> genus. The absence of effective vaccines and the limitations of current treatments make the search for effective therapies a real need. Different plant-derived essential oils (EOs) have shown antileishmanial effects, in particular from <i>Bixa orellana</i> L. (EO-Bo) and <i>Dysphania ambrosioides</i> (L.) Mosyakin & Clemants (EO-Da). In the present study, the EO-Bo and EO-Da, formulated in nanocochleates (EO-Bo-NC and EO-Da-NC, respectively), were evaluated in vitro and in vivo against <i>L. amazonensis</i>. The EO-Bo-NC and EO-Da-NC did not increase the in vitro inhibitory activity of the EOs, although the EO-Bo-NC showed reduced cytotoxic effects. In the animal model, both formulations (30 mg/kg/intralesional route/every 4 days/4 times) showed no deaths or weight loss greater than 10%. In the animal (mouse) model, EO-Bo-NC contributed to the control of infection (<i>p</i> < 0.05) in comparison with EO-Bo treatment, while the mice treated with EO-Da-NC exhibited larger lesions (<i>p</i> < 0.05) compared to those treated with EO-Da. The enhanced in vivo activity observed for EO-Bo-NC suggests that lipid-based nanoformulations like nanocochleates should be explored for their potential in the proper delivery of drugs, and in particular, the delivery of hydrophobic materials for effective cutaneous leishmaniasis treatment. |
| first_indexed | 2024-04-13T10:01:39Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T10:01:39Z |
| publishDate | 2019-11-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-42e0df094ff84d4fbc196ed243b221f12022-12-22T02:51:14ZengMDPI AGMolecules1420-30492019-11-012423422210.3390/molecules24234222molecules24234222<i>Bixa orellana</i> L. (Bixaceae) and <i>Dysphania ambrosioides</i> (L.) Mosyakin & Clemants (Amaranthaceae) Essential Oils Formulated in Nanocochleates against <i>Leishmania amazonensis</i>Laura Machín0Beatriz Tamargo1Abel Piñón2Regla C. Atíes3Ramón Scull4William N. Setzer5Lianet Monzote6Department of Pharmacy, Institute of Pharmacy and Food, Havana University, Havana 17100, CubaDepartment of Physiological Science, Latin American School of Medical Sciences, Havana 11300, CubaDepartment of Parasitology, Institute of Tropical Medicine Pedro Kourí, Havana 17100, CubaDepartment of Pharmacy, Institute of Pharmacy and Food, Havana University, Havana 17100, CubaDepartment of Pharmacy, Institute of Pharmacy and Food, Havana University, Havana 17100, CubaDepartment of Chemistry, University of Alabama in Huntsville, Huntsville, AL 35899, USADepartment of Parasitology, Institute of Tropical Medicine Pedro Kourí, Havana 17100, CubaLeishmaniasis is a group of neglected tropical diseases caused by protozoan parasites of the <i>Leishmania</i> genus. The absence of effective vaccines and the limitations of current treatments make the search for effective therapies a real need. Different plant-derived essential oils (EOs) have shown antileishmanial effects, in particular from <i>Bixa orellana</i> L. (EO-Bo) and <i>Dysphania ambrosioides</i> (L.) Mosyakin & Clemants (EO-Da). In the present study, the EO-Bo and EO-Da, formulated in nanocochleates (EO-Bo-NC and EO-Da-NC, respectively), were evaluated in vitro and in vivo against <i>L. amazonensis</i>. The EO-Bo-NC and EO-Da-NC did not increase the in vitro inhibitory activity of the EOs, although the EO-Bo-NC showed reduced cytotoxic effects. In the animal model, both formulations (30 mg/kg/intralesional route/every 4 days/4 times) showed no deaths or weight loss greater than 10%. In the animal (mouse) model, EO-Bo-NC contributed to the control of infection (<i>p</i> < 0.05) in comparison with EO-Bo treatment, while the mice treated with EO-Da-NC exhibited larger lesions (<i>p</i> < 0.05) compared to those treated with EO-Da. The enhanced in vivo activity observed for EO-Bo-NC suggests that lipid-based nanoformulations like nanocochleates should be explored for their potential in the proper delivery of drugs, and in particular, the delivery of hydrophobic materials for effective cutaneous leishmaniasis treatment.https://www.mdpi.com/1420-3049/24/23/4222<i>bixa orellana</i><i>chenopodium ambrosioides</i>essential oil<i>leishmania amazonensis</i>nanocochleate |
| spellingShingle | Laura Machín Beatriz Tamargo Abel Piñón Regla C. Atíes Ramón Scull William N. Setzer Lianet Monzote <i>Bixa orellana</i> L. (Bixaceae) and <i>Dysphania ambrosioides</i> (L.) Mosyakin & Clemants (Amaranthaceae) Essential Oils Formulated in Nanocochleates against <i>Leishmania amazonensis</i> Molecules <i>bixa orellana</i> <i>chenopodium ambrosioides</i> essential oil <i>leishmania amazonensis</i> nanocochleate |
| title | <i>Bixa orellana</i> L. (Bixaceae) and <i>Dysphania ambrosioides</i> (L.) Mosyakin & Clemants (Amaranthaceae) Essential Oils Formulated in Nanocochleates against <i>Leishmania amazonensis</i> |
| title_full | <i>Bixa orellana</i> L. (Bixaceae) and <i>Dysphania ambrosioides</i> (L.) Mosyakin & Clemants (Amaranthaceae) Essential Oils Formulated in Nanocochleates against <i>Leishmania amazonensis</i> |
| title_fullStr | <i>Bixa orellana</i> L. (Bixaceae) and <i>Dysphania ambrosioides</i> (L.) Mosyakin & Clemants (Amaranthaceae) Essential Oils Formulated in Nanocochleates against <i>Leishmania amazonensis</i> |
| title_full_unstemmed | <i>Bixa orellana</i> L. (Bixaceae) and <i>Dysphania ambrosioides</i> (L.) Mosyakin & Clemants (Amaranthaceae) Essential Oils Formulated in Nanocochleates against <i>Leishmania amazonensis</i> |
| title_short | <i>Bixa orellana</i> L. (Bixaceae) and <i>Dysphania ambrosioides</i> (L.) Mosyakin & Clemants (Amaranthaceae) Essential Oils Formulated in Nanocochleates against <i>Leishmania amazonensis</i> |
| title_sort | i bixa orellana i l bixaceae and i dysphania ambrosioides i l mosyakin amp clemants amaranthaceae essential oils formulated in nanocochleates against i leishmania amazonensis i |
| topic | <i>bixa orellana</i> <i>chenopodium ambrosioides</i> essential oil <i>leishmania amazonensis</i> nanocochleate |
| url | https://www.mdpi.com/1420-3049/24/23/4222 |
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