MicroRNA-206 Regulation of Skin Pigmentation in Koi Carp (Cyprinus carpio L.)
MicroRNAs (miRNAs) are ∼22 nucleotide non-coding RNA molecules that act as crucial roles in plenty of biological processes. However, the molecular and cellular mechanisms of miRNAs to regulate skin color differentiation and pigmentation in fish have not been fully understood. Herein, we revealed tha...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-02-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2020.00047/full |
| _version_ | 1811265911595728896 |
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| author | Zaijie Dong Zaijie Dong Mingkun Luo Lanmei Wang Haoran Yin Wenbin Zhu Jianjun Fu |
| author_facet | Zaijie Dong Zaijie Dong Mingkun Luo Lanmei Wang Haoran Yin Wenbin Zhu Jianjun Fu |
| author_sort | Zaijie Dong |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are ∼22 nucleotide non-coding RNA molecules that act as crucial roles in plenty of biological processes. However, the molecular and cellular mechanisms of miRNAs to regulate skin color differentiation and pigmentation in fish have not been fully understood. Herein, we revealed that miR-206, a skin-enriched miRNA, regulates melanocortin 1 receptor (Mc1r, a key regulator of melanogenesis) expression by binding to its 3'-untranslated (UTR) region through bioinformatics and luciferase reporter assay in koi carp (Cyprinus carpio L.). The analysis of spatial and temporal expression patterns suggested that miR-206 is a potential regulator in the skin pigmentation process. Then, we silenced it in vivo with an antagomir method. The result showed a substantial increase of Mc1r mRNA expression and protein level, and also its downstream genes: tyrosinase (Tyr) and dopachrome tautomerase (Dct) that encoding key enzymes involved in melanin synthesis. Moreover, we constructed the miRNA-206 sponge lentivirus vector to transfect koi carp melanocytes in vitro, further checked the functions of melanocytes using Cck-8 and Transwell assays. As a result, inhibition of miR-206 significantly up-regulated Mc1r mRNA expression and protein level and accelerated the melanocyte proliferation and migration ability compared with the scrambled-sequence negative control group (miR-NC). Overall, these findings provide the evidence that miR-206 plays a regulatory role in the skin color pigmentation through targeting the Mc1r gene and would facilitate understanding the molecular regulatory mechanisms underlying miRNA-mediated skin color pigmentation in koi carp. |
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| id | doaj.art-42e0e6b0801146a3a474b49f770bf525 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-12T20:32:30Z |
| publishDate | 2020-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Genetics |
| spelling | doaj.art-42e0e6b0801146a3a474b49f770bf5252022-12-22T03:17:41ZengFrontiers Media S.A.Frontiers in Genetics1664-80212020-02-011110.3389/fgene.2020.00047495756MicroRNA-206 Regulation of Skin Pigmentation in Koi Carp (Cyprinus carpio L.)Zaijie Dong0Zaijie Dong1Mingkun Luo2Lanmei Wang3Haoran Yin4Wenbin Zhu5Jianjun Fu6Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Jiangsu, ChinaWuxi Fisheries College, Nanjing Agricultural University, Jiangsu, ChinaWuxi Fisheries College, Nanjing Agricultural University, Jiangsu, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Jiangsu, ChinaWuxi Fisheries College, Nanjing Agricultural University, Jiangsu, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Jiangsu, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences, Ministry of Agriculture and Rural Affairs, Jiangsu, ChinaMicroRNAs (miRNAs) are ∼22 nucleotide non-coding RNA molecules that act as crucial roles in plenty of biological processes. However, the molecular and cellular mechanisms of miRNAs to regulate skin color differentiation and pigmentation in fish have not been fully understood. Herein, we revealed that miR-206, a skin-enriched miRNA, regulates melanocortin 1 receptor (Mc1r, a key regulator of melanogenesis) expression by binding to its 3'-untranslated (UTR) region through bioinformatics and luciferase reporter assay in koi carp (Cyprinus carpio L.). The analysis of spatial and temporal expression patterns suggested that miR-206 is a potential regulator in the skin pigmentation process. Then, we silenced it in vivo with an antagomir method. The result showed a substantial increase of Mc1r mRNA expression and protein level, and also its downstream genes: tyrosinase (Tyr) and dopachrome tautomerase (Dct) that encoding key enzymes involved in melanin synthesis. Moreover, we constructed the miRNA-206 sponge lentivirus vector to transfect koi carp melanocytes in vitro, further checked the functions of melanocytes using Cck-8 and Transwell assays. As a result, inhibition of miR-206 significantly up-regulated Mc1r mRNA expression and protein level and accelerated the melanocyte proliferation and migration ability compared with the scrambled-sequence negative control group (miR-NC). Overall, these findings provide the evidence that miR-206 plays a regulatory role in the skin color pigmentation through targeting the Mc1r gene and would facilitate understanding the molecular regulatory mechanisms underlying miRNA-mediated skin color pigmentation in koi carp.https://www.frontiersin.org/article/10.3389/fgene.2020.00047/fullMiRNA-206Mc1rskin colormelanocyteskoi carp |
| spellingShingle | Zaijie Dong Zaijie Dong Mingkun Luo Lanmei Wang Haoran Yin Wenbin Zhu Jianjun Fu MicroRNA-206 Regulation of Skin Pigmentation in Koi Carp (Cyprinus carpio L.) Frontiers in Genetics MiRNA-206 Mc1r skin color melanocytes koi carp |
| title | MicroRNA-206 Regulation of Skin Pigmentation in Koi Carp (Cyprinus carpio L.) |
| title_full | MicroRNA-206 Regulation of Skin Pigmentation in Koi Carp (Cyprinus carpio L.) |
| title_fullStr | MicroRNA-206 Regulation of Skin Pigmentation in Koi Carp (Cyprinus carpio L.) |
| title_full_unstemmed | MicroRNA-206 Regulation of Skin Pigmentation in Koi Carp (Cyprinus carpio L.) |
| title_short | MicroRNA-206 Regulation of Skin Pigmentation in Koi Carp (Cyprinus carpio L.) |
| title_sort | microrna 206 regulation of skin pigmentation in koi carp cyprinus carpio l |
| topic | MiRNA-206 Mc1r skin color melanocytes koi carp |
| url | https://www.frontiersin.org/article/10.3389/fgene.2020.00047/full |
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