Effect of Ultraviolet Light C (UV-C) Radiation Generated by Semiconductor Light Sources on Human <i>Beta</i>-Coronaviruses’ Inactivation
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has completely disrupted people’s lives. All over the world, many restrictions and precautions have been introduced to reduce the spread of coronavirus disease 2019 (COVID-19). Ultraviolet C (UV-C) radiation is widely used to...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/15/6/2302 |
| _version_ | 1797445530059538432 |
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| author | Piotr Sobotka Maciej Przychodzki Konrad Uściło Tomasz R. Woliński Monika Staniszewska |
| author_facet | Piotr Sobotka Maciej Przychodzki Konrad Uściło Tomasz R. Woliński Monika Staniszewska |
| author_sort | Piotr Sobotka |
| collection | DOAJ |
| description | The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has completely disrupted people’s lives. All over the world, many restrictions and precautions have been introduced to reduce the spread of coronavirus disease 2019 (COVID-19). Ultraviolet C (UV-C) radiation is widely used to disinfect rooms, surfaces, and medical tools; however, this paper presents novel results obtained for modern UV-C light-emitting diodes (LEDs), examining their effect on inhibiting the multiplication of viruses. The main goal of the work was to investigate how to most effectively use UV-C LEDs to inactivate viruses. We showed that UV-C radiation operating at a 275 nm wavelength is optimal for germicidal effectiveness in a time exposure (25–48 s) study: >3 log-reduction with the Kärber method and >6 log-reduction with UV spectrophotometry were noted. We used real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) to reliably estimate virus infectivity reduction after 275 nm UV-C disinfection. The relative quantification (RQ) of infectious particles detected after 40–48 s distinctly decreased. The irradiated viral RNAs were underexpressed compared to the untreated control virial amplicon (estimated as RQ = 1). In conclusion, this work provides the first experimental data on 275 nm UV-C in the inactivation of human coronavirus OC43 (HoV-OC43), showing the most potent germicidal effect without hazardous effect. |
| first_indexed | 2024-03-09T13:27:07Z |
| format | Article |
| id | doaj.art-42e10fffdacf4662aedadf40f48b5226 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T13:27:07Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-42e10fffdacf4662aedadf40f48b52262023-11-30T21:22:31ZengMDPI AGMaterials1996-19442022-03-01156230210.3390/ma15062302Effect of Ultraviolet Light C (UV-C) Radiation Generated by Semiconductor Light Sources on Human <i>Beta</i>-Coronaviruses’ InactivationPiotr Sobotka0Maciej Przychodzki1Konrad Uściło2Tomasz R. Woliński3Monika Staniszewska4Faculty of Physics, Warsaw University of Technology, 00-662 Warsaw, PolandFaculty of Physics, Warsaw University of Technology, 00-662 Warsaw, PolandCentre for Advanced Materials and Technologies CEZAMAT, Warsaw University of Technology, 02-822 Warsaw, PolandFaculty of Physics, Warsaw University of Technology, 00-662 Warsaw, PolandCentre for Advanced Materials and Technologies CEZAMAT, Warsaw University of Technology, 02-822 Warsaw, PolandThe severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has completely disrupted people’s lives. All over the world, many restrictions and precautions have been introduced to reduce the spread of coronavirus disease 2019 (COVID-19). Ultraviolet C (UV-C) radiation is widely used to disinfect rooms, surfaces, and medical tools; however, this paper presents novel results obtained for modern UV-C light-emitting diodes (LEDs), examining their effect on inhibiting the multiplication of viruses. The main goal of the work was to investigate how to most effectively use UV-C LEDs to inactivate viruses. We showed that UV-C radiation operating at a 275 nm wavelength is optimal for germicidal effectiveness in a time exposure (25–48 s) study: >3 log-reduction with the Kärber method and >6 log-reduction with UV spectrophotometry were noted. We used real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) to reliably estimate virus infectivity reduction after 275 nm UV-C disinfection. The relative quantification (RQ) of infectious particles detected after 40–48 s distinctly decreased. The irradiated viral RNAs were underexpressed compared to the untreated control virial amplicon (estimated as RQ = 1). In conclusion, this work provides the first experimental data on 275 nm UV-C in the inactivation of human coronavirus OC43 (HoV-OC43), showing the most potent germicidal effect without hazardous effect.https://www.mdpi.com/1996-1944/15/6/2302human coronavirus OC43 (HCoV-OC43)severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)coronavirus disease 2019 (COVID-19)ultraviolet C (UV-C)light-emitting diode (LED)virus inactivation |
| spellingShingle | Piotr Sobotka Maciej Przychodzki Konrad Uściło Tomasz R. Woliński Monika Staniszewska Effect of Ultraviolet Light C (UV-C) Radiation Generated by Semiconductor Light Sources on Human <i>Beta</i>-Coronaviruses’ Inactivation Materials human coronavirus OC43 (HCoV-OC43) severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) coronavirus disease 2019 (COVID-19) ultraviolet C (UV-C) light-emitting diode (LED) virus inactivation |
| title | Effect of Ultraviolet Light C (UV-C) Radiation Generated by Semiconductor Light Sources on Human <i>Beta</i>-Coronaviruses’ Inactivation |
| title_full | Effect of Ultraviolet Light C (UV-C) Radiation Generated by Semiconductor Light Sources on Human <i>Beta</i>-Coronaviruses’ Inactivation |
| title_fullStr | Effect of Ultraviolet Light C (UV-C) Radiation Generated by Semiconductor Light Sources on Human <i>Beta</i>-Coronaviruses’ Inactivation |
| title_full_unstemmed | Effect of Ultraviolet Light C (UV-C) Radiation Generated by Semiconductor Light Sources on Human <i>Beta</i>-Coronaviruses’ Inactivation |
| title_short | Effect of Ultraviolet Light C (UV-C) Radiation Generated by Semiconductor Light Sources on Human <i>Beta</i>-Coronaviruses’ Inactivation |
| title_sort | effect of ultraviolet light c uv c radiation generated by semiconductor light sources on human i beta i coronaviruses inactivation |
| topic | human coronavirus OC43 (HCoV-OC43) severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) coronavirus disease 2019 (COVID-19) ultraviolet C (UV-C) light-emitting diode (LED) virus inactivation |
| url | https://www.mdpi.com/1996-1944/15/6/2302 |
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