Metabolomic and regular analysis reveal phytotoxic mechanisms of sterigmatocystin in Amaranthus retroflexus L.
Sterigmatocystin (STE) is a common hepatotoxic and nephrotoxic contaminant in cereals, however, its phytotoxicity and mechanisms are poorly understood. Here, the phytotoxic mechanisms of STE were investigated via the metabolomics of Amaranthus retroflexus L. A total of 140 and 113 differential metabo...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322011137 |
| _version_ | 1811312346098827264 |
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| author | Dong-Lin Zhao Rui-Xue Sun Xiao-Bin Han Mei Wang Xi-Fen Zhang Xian-Bo Wang Jun Wan Jing Liu Yi-Qiang Li Si-Qi Ma Cheng-Sheng Zhang |
| author_facet | Dong-Lin Zhao Rui-Xue Sun Xiao-Bin Han Mei Wang Xi-Fen Zhang Xian-Bo Wang Jun Wan Jing Liu Yi-Qiang Li Si-Qi Ma Cheng-Sheng Zhang |
| author_sort | Dong-Lin Zhao |
| collection | DOAJ |
| description | Sterigmatocystin (STE) is a common hepatotoxic and nephrotoxic contaminant in cereals, however, its phytotoxicity and mechanisms are poorly understood. Here, the phytotoxic mechanisms of STE were investigated via the metabolomics of Amaranthus retroflexus L. A total of 140 and 113 differential metabolites were detected in the leaves and stems, respectively, among which amino acids, lipids, and phenolic compounds were significantly perturbed. Valine, leucine, isoleucine, and lysine biosynthesis were affected by STE. These metabolic responses revealed that STE might be toxic to plants by altering the plasma membrane and inducing oxidative damage, which was verified by measuring the relative electrical conductivity and quantification of reactive oxygen species. The elevated amino acids, as well as the decreased of D-sedoheptuiose-7-phosphate indicated increased proteolysis and carbohydrate metabolism restriction. Furthermore, the IAA level also decreased. This study provides a better understanding of the impacts of STE on the public health, environment and food security. |
| first_indexed | 2024-04-13T10:35:51Z |
| format | Article |
| id | doaj.art-42e2ce6250d6483db05019fe90ed0c50 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-13T10:35:51Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-42e2ce6250d6483db05019fe90ed0c502022-12-22T02:50:04ZengElsevierEcotoxicology and Environmental Safety0147-65132022-12-01247114273Metabolomic and regular analysis reveal phytotoxic mechanisms of sterigmatocystin in Amaranthus retroflexus L.Dong-Lin Zhao0Rui-Xue Sun1Xiao-Bin Han2Mei Wang3Xi-Fen Zhang4Xian-Bo Wang5Jun Wan6Jing Liu7Yi-Qiang Li8Si-Qi Ma9Cheng-Sheng Zhang10Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaTobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaZunyi Branch, Guizhou Tobacco Company, Zunyi 563000, ChinaTobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaTobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaZunyi Branch, Guizhou Tobacco Company, Zunyi 563000, ChinaZunyi Branch, Guizhou Tobacco Company, Zunyi 563000, ChinaZunyi Branch, Guizhou Tobacco Company, Zunyi 563000, ChinaTobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, ChinaTobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China; Corresponding authors.Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China; Corresponding authors.Sterigmatocystin (STE) is a common hepatotoxic and nephrotoxic contaminant in cereals, however, its phytotoxicity and mechanisms are poorly understood. Here, the phytotoxic mechanisms of STE were investigated via the metabolomics of Amaranthus retroflexus L. A total of 140 and 113 differential metabolites were detected in the leaves and stems, respectively, among which amino acids, lipids, and phenolic compounds were significantly perturbed. Valine, leucine, isoleucine, and lysine biosynthesis were affected by STE. These metabolic responses revealed that STE might be toxic to plants by altering the plasma membrane and inducing oxidative damage, which was verified by measuring the relative electrical conductivity and quantification of reactive oxygen species. The elevated amino acids, as well as the decreased of D-sedoheptuiose-7-phosphate indicated increased proteolysis and carbohydrate metabolism restriction. Furthermore, the IAA level also decreased. This study provides a better understanding of the impacts of STE on the public health, environment and food security.http://www.sciencedirect.com/science/article/pii/S0147651322011137SterigmatocystinPhytotoxic mechanismsAmino acid biosynthesisCell membrane alterationsOxidative damage |
| spellingShingle | Dong-Lin Zhao Rui-Xue Sun Xiao-Bin Han Mei Wang Xi-Fen Zhang Xian-Bo Wang Jun Wan Jing Liu Yi-Qiang Li Si-Qi Ma Cheng-Sheng Zhang Metabolomic and regular analysis reveal phytotoxic mechanisms of sterigmatocystin in Amaranthus retroflexus L. Ecotoxicology and Environmental Safety Sterigmatocystin Phytotoxic mechanisms Amino acid biosynthesis Cell membrane alterations Oxidative damage |
| title | Metabolomic and regular analysis reveal phytotoxic mechanisms of sterigmatocystin in Amaranthus retroflexus L. |
| title_full | Metabolomic and regular analysis reveal phytotoxic mechanisms of sterigmatocystin in Amaranthus retroflexus L. |
| title_fullStr | Metabolomic and regular analysis reveal phytotoxic mechanisms of sterigmatocystin in Amaranthus retroflexus L. |
| title_full_unstemmed | Metabolomic and regular analysis reveal phytotoxic mechanisms of sterigmatocystin in Amaranthus retroflexus L. |
| title_short | Metabolomic and regular analysis reveal phytotoxic mechanisms of sterigmatocystin in Amaranthus retroflexus L. |
| title_sort | metabolomic and regular analysis reveal phytotoxic mechanisms of sterigmatocystin in amaranthus retroflexus l |
| topic | Sterigmatocystin Phytotoxic mechanisms Amino acid biosynthesis Cell membrane alterations Oxidative damage |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322011137 |
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