Identification of male genotypes in Pistacia vera L. species using SSR markers
<p class="Default">Genetic identification among different 15 male genotypes of <em>Pistacia vera </em>L. species in comparison with five main commercial male cultivars accredited by Ministry of Agriculture (Adam, Jamil, Ebrahim, Elyas and Khalifa) was achieved using SSR m...
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| Format: | Article |
| Language: | English |
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Progressive Sustainable Developers Nepal
2017-05-01
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| Series: | International Journal of Environment |
| Subjects: | |
| Online Access: | http://nepjol.info/index.php/IJE/article/view/17360 |
| _version_ | 1818983924113604608 |
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| author | Najwa Motaeb Alhajjar Bayan Mohammed Muzher |
| author_facet | Najwa Motaeb Alhajjar Bayan Mohammed Muzher |
| author_sort | Najwa Motaeb Alhajjar |
| collection | DOAJ |
| description | <p class="Default">Genetic identification among different 15 male genotypes of <em>Pistacia vera </em>L. species in comparison with five main commercial male cultivars accredited by Ministry of Agriculture (Adam, Jamil, Ebrahim, Elyas and Khalifa) was achieved using SSR markers. Seventeen primer pairs out of Twenty SSR primers were able to amplify PCR products. SSR segregation produced 44 putative alleles, of which 34 were polymorphic (77.27%). Genetic similarity among all studied genotypes ranged from 0.45 between Jamil cultivar and MAS1 genotype to 1 between Ebrahim cultivar and MA1 genotype which means that they are identical. The UPGMA cluster analysis based on Jaccard’s coefficient grouped all genotypes into three main clusters. The number of alleles revealed by each SSR analysis ranged from 1 to 5, with a high level of expected heterozygosity (He) 0.507. Co-dominant SSRs loci were observed in some studied genotypes giving a value 0.235 of observed heterozygosity (Ho). According to the polymorphic allele’s number and the expected heterozygosity; Marker Index (MI) was 23.97. Our results concluded that SSR marker is an informative technique, which revealed high ability to differentiate individuals, and played an important role as a genetic marker for identification and evaluation studies within <em>P. vera </em>species.</p><p class="Default"><strong>INTERNATIONAL JOURNAL OF ENVIRONMEN</strong>T</p><p>Volume-6, Issue-2, Mar-May 2017, Page: 30-42</p> |
| first_indexed | 2024-12-20T18:10:50Z |
| format | Article |
| id | doaj.art-42e4875e34944852b9422e3be81b0c8b |
| institution | Directory Open Access Journal |
| issn | 2091-2854 |
| language | English |
| last_indexed | 2024-12-20T18:10:50Z |
| publishDate | 2017-05-01 |
| publisher | Progressive Sustainable Developers Nepal |
| record_format | Article |
| series | International Journal of Environment |
| spelling | doaj.art-42e4875e34944852b9422e3be81b0c8b2022-12-21T19:30:28ZengProgressive Sustainable Developers NepalInternational Journal of Environment2091-28542017-05-0162304210.3126/ije.v6i2.1736013484Identification of male genotypes in Pistacia vera L. species using SSR markersNajwa Motaeb Alhajjar0Bayan Mohammed Muzher1General Commission for Scientific Agricultural ResearchGeneral Commission for Scientific Agricultural Research<p class="Default">Genetic identification among different 15 male genotypes of <em>Pistacia vera </em>L. species in comparison with five main commercial male cultivars accredited by Ministry of Agriculture (Adam, Jamil, Ebrahim, Elyas and Khalifa) was achieved using SSR markers. Seventeen primer pairs out of Twenty SSR primers were able to amplify PCR products. SSR segregation produced 44 putative alleles, of which 34 were polymorphic (77.27%). Genetic similarity among all studied genotypes ranged from 0.45 between Jamil cultivar and MAS1 genotype to 1 between Ebrahim cultivar and MA1 genotype which means that they are identical. The UPGMA cluster analysis based on Jaccard’s coefficient grouped all genotypes into three main clusters. The number of alleles revealed by each SSR analysis ranged from 1 to 5, with a high level of expected heterozygosity (He) 0.507. Co-dominant SSRs loci were observed in some studied genotypes giving a value 0.235 of observed heterozygosity (Ho). According to the polymorphic allele’s number and the expected heterozygosity; Marker Index (MI) was 23.97. Our results concluded that SSR marker is an informative technique, which revealed high ability to differentiate individuals, and played an important role as a genetic marker for identification and evaluation studies within <em>P. vera </em>species.</p><p class="Default"><strong>INTERNATIONAL JOURNAL OF ENVIRONMEN</strong>T</p><p>Volume-6, Issue-2, Mar-May 2017, Page: 30-42</p>http://nepjol.info/index.php/IJE/article/view/17360Pistacia vera L.SSR markersGenetic similarityExpected heterozygosityObserved heterozygosity |
| spellingShingle | Najwa Motaeb Alhajjar Bayan Mohammed Muzher Identification of male genotypes in Pistacia vera L. species using SSR markers International Journal of Environment Pistacia vera L. SSR markers Genetic similarity Expected heterozygosity Observed heterozygosity |
| title | Identification of male genotypes in Pistacia vera L. species using SSR markers |
| title_full | Identification of male genotypes in Pistacia vera L. species using SSR markers |
| title_fullStr | Identification of male genotypes in Pistacia vera L. species using SSR markers |
| title_full_unstemmed | Identification of male genotypes in Pistacia vera L. species using SSR markers |
| title_short | Identification of male genotypes in Pistacia vera L. species using SSR markers |
| title_sort | identification of male genotypes in pistacia vera l species using ssr markers |
| topic | Pistacia vera L. SSR markers Genetic similarity Expected heterozygosity Observed heterozygosity |
| url | http://nepjol.info/index.php/IJE/article/view/17360 |
| work_keys_str_mv | AT najwamotaebalhajjar identificationofmalegenotypesinpistaciaveralspeciesusingssrmarkers AT bayanmohammedmuzher identificationofmalegenotypesinpistaciaveralspeciesusingssrmarkers |