Isolation and Identification of Carcinogen Acenaphthene-Degrading Endemic Bacteria from Crude Oil Contaminated Soils around Abadan Refinery
Background and Objective: PAHs are non-polar organic compounds consisting of two or more fused benzene multi-rings. Among these compounds, acenaphthene is a multi-ring hydrocarbon that occurs abundantly in nature. Use of microorganisms to clean the contaminations of soil can be cheap and effective....
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| Format: | Article |
| Language: | fas |
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Fasa University of Medical Sciences
2012-12-01
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| Series: | Journal of Fasa University of Medical Sciences |
| Subjects: | |
| Online Access: | http://journal.fums.ac.ir/browse.php?a_code=A-10-26-4&slc_lang=en&sid=1 |
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| author | Farshid Kafilzadeh Seyedeh Zeynab Hoseyni Peyman Izedpanah Houshang Jamali |
| author_facet | Farshid Kafilzadeh Seyedeh Zeynab Hoseyni Peyman Izedpanah Houshang Jamali |
| author_sort | Farshid Kafilzadeh |
| collection | DOAJ |
| description | Background and Objective: PAHs are non-polar organic compounds consisting of two or more fused benzene multi-rings. Among these compounds, acenaphthene is a multi-ring hydrocarbon that occurs abundantly in nature. Use of microorganisms to clean the contaminations of soil can be cheap and effective. The most important acenaphthene-degrading bacteria are pseudomonas, micrococcus, and Bacillus. The goal of this study was to isolate and identify the bacteria which degrade acenaphthene in soils around Abadan Refinery and to investigate the relation between the levels of environmental pollution with acenaphthene. Materials and Methods: Soil samples were collected from three areas around Abadan Refinery. The number of the bacteria was counted on the nutrient agar culture with and without acenaphthene. Isolation of the bacteria was done by culturing the samples on acenaphthene broth with a mineral-salt medium, and on an acenaphthene agar medium. Then, the bacteria were identified via biochemical diagnostic tests. Results: The logarithm average of the bacteria was 4.786 ± 0.073 at a medium with acenaphthene, which was 6.671 ± 0.073 less than that of the control medium. The maximum number of degrading bacteria was 7.089 ± 0.089 at Station C, and the minimum number of the degrading bacteria was 4.485 ± 0.089 at Station B. In this study, Bacillus sp, Micrococcus Luteus, Corynebacterium sp, Staphylococcus epidermidis, and Pseudomonas sp bacteria were isolated and identified in terms of frequency, respectively. Conclusion: The results of this study showed that the soil around Abadan Refinery contained a great number of acenaphthene degrading bacteria, especially Bacillus and Micrococcus. |
| first_indexed | 2024-04-13T06:11:36Z |
| format | Article |
| id | doaj.art-42e60c8a203e45698f0f79d6f822acec |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2228-7329 |
| language | fas |
| last_indexed | 2024-04-13T06:11:36Z |
| publishDate | 2012-12-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Fasa University of Medical Sciences |
| spelling | doaj.art-42e60c8a203e45698f0f79d6f822acec2022-12-22T02:59:01ZfasFasa University of Medical SciencesJournal of Fasa University of Medical Sciences2228-51052228-73292012-12-0123181186Isolation and Identification of Carcinogen Acenaphthene-Degrading Endemic Bacteria from Crude Oil Contaminated Soils around Abadan RefineryFarshid KafilzadehSeyedeh Zeynab HoseyniPeyman IzedpanahHoushang JamaliBackground and Objective: PAHs are non-polar organic compounds consisting of two or more fused benzene multi-rings. Among these compounds, acenaphthene is a multi-ring hydrocarbon that occurs abundantly in nature. Use of microorganisms to clean the contaminations of soil can be cheap and effective. The most important acenaphthene-degrading bacteria are pseudomonas, micrococcus, and Bacillus. The goal of this study was to isolate and identify the bacteria which degrade acenaphthene in soils around Abadan Refinery and to investigate the relation between the levels of environmental pollution with acenaphthene. Materials and Methods: Soil samples were collected from three areas around Abadan Refinery. The number of the bacteria was counted on the nutrient agar culture with and without acenaphthene. Isolation of the bacteria was done by culturing the samples on acenaphthene broth with a mineral-salt medium, and on an acenaphthene agar medium. Then, the bacteria were identified via biochemical diagnostic tests. Results: The logarithm average of the bacteria was 4.786 ± 0.073 at a medium with acenaphthene, which was 6.671 ± 0.073 less than that of the control medium. The maximum number of degrading bacteria was 7.089 ± 0.089 at Station C, and the minimum number of the degrading bacteria was 4.485 ± 0.089 at Station B. In this study, Bacillus sp, Micrococcus Luteus, Corynebacterium sp, Staphylococcus epidermidis, and Pseudomonas sp bacteria were isolated and identified in terms of frequency, respectively. Conclusion: The results of this study showed that the soil around Abadan Refinery contained a great number of acenaphthene degrading bacteria, especially Bacillus and Micrococcus.http://journal.fums.ac.ir/browse.php?a_code=A-10-26-4&slc_lang=en&sid=1AcenaphtheneDegraderBacillusMicrococcusAbadan refinery |
| spellingShingle | Farshid Kafilzadeh Seyedeh Zeynab Hoseyni Peyman Izedpanah Houshang Jamali Isolation and Identification of Carcinogen Acenaphthene-Degrading Endemic Bacteria from Crude Oil Contaminated Soils around Abadan Refinery Journal of Fasa University of Medical Sciences Acenaphthene Degrader Bacillus Micrococcus Abadan refinery |
| title | Isolation and Identification of Carcinogen Acenaphthene-Degrading Endemic Bacteria from Crude Oil Contaminated Soils around Abadan Refinery |
| title_full | Isolation and Identification of Carcinogen Acenaphthene-Degrading Endemic Bacteria from Crude Oil Contaminated Soils around Abadan Refinery |
| title_fullStr | Isolation and Identification of Carcinogen Acenaphthene-Degrading Endemic Bacteria from Crude Oil Contaminated Soils around Abadan Refinery |
| title_full_unstemmed | Isolation and Identification of Carcinogen Acenaphthene-Degrading Endemic Bacteria from Crude Oil Contaminated Soils around Abadan Refinery |
| title_short | Isolation and Identification of Carcinogen Acenaphthene-Degrading Endemic Bacteria from Crude Oil Contaminated Soils around Abadan Refinery |
| title_sort | isolation and identification of carcinogen acenaphthene degrading endemic bacteria from crude oil contaminated soils around abadan refinery |
| topic | Acenaphthene Degrader Bacillus Micrococcus Abadan refinery |
| url | http://journal.fums.ac.ir/browse.php?a_code=A-10-26-4&slc_lang=en&sid=1 |
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