Perfusion-based co-culture model system for bone tissue engineering
In this work, we report on a perfusion-based co-culture system that could be used for bone tissue engineering applications. The model system is created using a combination of Primary Human Umbilical Vein Endothelial Cells (HUVECs) and osteoblast-like Saos-2 cells encapsulated within a Gelatin Methac...
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| Format: | Article |
| Language: | English |
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AIMS Press
2020-07-01
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| Series: | AIMS Bioengineering |
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| Online Access: | https://www.aimspress.com/article/10.3934/bioeng.2020009/fulltext.html |
| _version_ | 1818265617457741824 |
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| author | Stephen W. Sawyer Stephen W. Sawyer Stephen W. Sawyer Stephen W. Sawyer |
| author_facet | Stephen W. Sawyer Stephen W. Sawyer Stephen W. Sawyer Stephen W. Sawyer |
| author_sort | Stephen W. Sawyer |
| collection | DOAJ |
| description | In this work, we report on a perfusion-based co-culture system that could be used for bone tissue engineering applications. The model system is created using a combination of Primary Human Umbilical Vein Endothelial Cells (HUVECs) and osteoblast-like Saos-2 cells encapsulated within a Gelatin Methacrylate (GelMA)-collagen hydrogel blend contained within 3D printed, perfusable constructs. The constructs contain dual channels, within a custom-built bioreactor, that were perfused with osteogenic media for up to two weeks in order to induce mineral deposition. Mineral deposition in constructs containing only HUVECs, only Saos-2 cells, or a combination thereof was quantified by microCT to determine if the combination of endothelial cells and bone-like cells increased mineral deposition. Histological and fluorescent staining was used to verify mineral deposition and cellular function both along and between the perfused channels. While there was not a quantifiable difference in the amount of mineral deposited in Saos-2 only versus Saos-2 plus HUVEC samples, the location of the deposited mineral differed dramatically between the groups and indicated that the addition of HUVECs within the GelMA matrix allowed Saos-2 cells, in diffusion limited regions of the construct, to deposit bone mineral. This work serves as a model on how to create perfusable bone tissue engineering constructs using a combination of 3D printing and cellular co-cultures. |
| first_indexed | 2024-12-12T19:53:39Z |
| format | Article |
| id | doaj.art-42e817dc75344db5b5cd0c27fdcb758a |
| institution | Directory Open Access Journal |
| issn | 2375-1487 2375-1495 |
| language | English |
| last_indexed | 2024-12-12T19:53:39Z |
| publishDate | 2020-07-01 |
| publisher | AIMS Press |
| record_format | Article |
| series | AIMS Bioengineering |
| spelling | doaj.art-42e817dc75344db5b5cd0c27fdcb758a2022-12-22T00:13:56ZengAIMS PressAIMS Bioengineering2375-14872375-14952020-07-01729110510.3934/bioeng.2020009Perfusion-based co-culture model system for bone tissue engineeringStephen W. Sawyer0Stephen W. Sawyer1Stephen W. Sawyer2Stephen W. Sawyer31 Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY, USA1 Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY, USA2 Department of Orthopedic Surgery, SUNY Upstate Medical University, Syracuse, NY, USA1 Department of Biomedical and Chemical Engineering, Syracuse University, Syracuse, NY, USAIn this work, we report on a perfusion-based co-culture system that could be used for bone tissue engineering applications. The model system is created using a combination of Primary Human Umbilical Vein Endothelial Cells (HUVECs) and osteoblast-like Saos-2 cells encapsulated within a Gelatin Methacrylate (GelMA)-collagen hydrogel blend contained within 3D printed, perfusable constructs. The constructs contain dual channels, within a custom-built bioreactor, that were perfused with osteogenic media for up to two weeks in order to induce mineral deposition. Mineral deposition in constructs containing only HUVECs, only Saos-2 cells, or a combination thereof was quantified by microCT to determine if the combination of endothelial cells and bone-like cells increased mineral deposition. Histological and fluorescent staining was used to verify mineral deposition and cellular function both along and between the perfused channels. While there was not a quantifiable difference in the amount of mineral deposited in Saos-2 only versus Saos-2 plus HUVEC samples, the location of the deposited mineral differed dramatically between the groups and indicated that the addition of HUVECs within the GelMA matrix allowed Saos-2 cells, in diffusion limited regions of the construct, to deposit bone mineral. This work serves as a model on how to create perfusable bone tissue engineering constructs using a combination of 3D printing and cellular co-cultures.https://www.aimspress.com/article/10.3934/bioeng.2020009/fulltext.htmlgelatin methacrylatemineral formationcell encapsulationperfusionbioreactorendothelial cells |
| spellingShingle | Stephen W. Sawyer Stephen W. Sawyer Stephen W. Sawyer Stephen W. Sawyer Perfusion-based co-culture model system for bone tissue engineering AIMS Bioengineering gelatin methacrylate mineral formation cell encapsulation perfusion bioreactor endothelial cells |
| title | Perfusion-based co-culture model system for bone tissue engineering |
| title_full | Perfusion-based co-culture model system for bone tissue engineering |
| title_fullStr | Perfusion-based co-culture model system for bone tissue engineering |
| title_full_unstemmed | Perfusion-based co-culture model system for bone tissue engineering |
| title_short | Perfusion-based co-culture model system for bone tissue engineering |
| title_sort | perfusion based co culture model system for bone tissue engineering |
| topic | gelatin methacrylate mineral formation cell encapsulation perfusion bioreactor endothelial cells |
| url | https://www.aimspress.com/article/10.3934/bioeng.2020009/fulltext.html |
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