<i>Myxococcus xanthus</i> Encapsulin as a Promising Platform for Intracellular Protein Delivery
Introducing a new genetically encoded material containing a photoactivatable label as a model cargo protein, based on <i>Myxococcus xanthus</i> (<i>Mx</i>) encapsulin system stably expressed in human 293T cells. Encapsulin from <i>Mx</i> is known to be a protein-b...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/24/15591 |
| Summary: | Introducing a new genetically encoded material containing a photoactivatable label as a model cargo protein, based on <i>Myxococcus xanthus</i> (<i>Mx</i>) encapsulin system stably expressed in human 293T cells. Encapsulin from <i>Mx</i> is known to be a protein-based container for a ferritin-like cargo in its shell which could be replaced with an exogenous cargo protein, resulting in a modified encapsulin system. We replaced <i>Mx</i> natural cargo with a foreign photoactivatable mCherry (PAmCherry) fluorescent protein and isolated encapsulins, containing PAmCherry, from 293T cells. Isolated <i>Mx</i> encapsulin shells containing photoactivatable label can be internalized by macrophages, wherein the PAmCherry fluorescent signal remains clearly visible. We believe that a genetically encoded nanocarrier system obtained in this study, can be used as a platform for controllable delivery of protein/peptide therapeutics in vitro. |
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| ISSN: | 1661-6596 1422-0067 |