<i>Myxococcus xanthus</i> Encapsulin as a Promising Platform for Intracellular Protein Delivery
Introducing a new genetically encoded material containing a photoactivatable label as a model cargo protein, based on <i>Myxococcus xanthus</i> (<i>Mx</i>) encapsulin system stably expressed in human 293T cells. Encapsulin from <i>Mx</i> is known to be a protein-b...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/24/15591 |
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| author | Anna N. Gabashvili Nelly S. Chmelyuk Viktoria A. Sarkisova Pavel A. Melnikov Alevtina S. Semkina Aleksey A. Nikitin Maxim A. Abakumov |
| author_facet | Anna N. Gabashvili Nelly S. Chmelyuk Viktoria A. Sarkisova Pavel A. Melnikov Alevtina S. Semkina Aleksey A. Nikitin Maxim A. Abakumov |
| author_sort | Anna N. Gabashvili |
| collection | DOAJ |
| description | Introducing a new genetically encoded material containing a photoactivatable label as a model cargo protein, based on <i>Myxococcus xanthus</i> (<i>Mx</i>) encapsulin system stably expressed in human 293T cells. Encapsulin from <i>Mx</i> is known to be a protein-based container for a ferritin-like cargo in its shell which could be replaced with an exogenous cargo protein, resulting in a modified encapsulin system. We replaced <i>Mx</i> natural cargo with a foreign photoactivatable mCherry (PAmCherry) fluorescent protein and isolated encapsulins, containing PAmCherry, from 293T cells. Isolated <i>Mx</i> encapsulin shells containing photoactivatable label can be internalized by macrophages, wherein the PAmCherry fluorescent signal remains clearly visible. We believe that a genetically encoded nanocarrier system obtained in this study, can be used as a platform for controllable delivery of protein/peptide therapeutics in vitro. |
| first_indexed | 2024-03-09T16:19:34Z |
| format | Article |
| id | doaj.art-42e89847e844484696824f072dfd4ee8 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:19:34Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-42e89847e844484696824f072dfd4ee82023-11-24T15:24:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0123241559110.3390/ijms232415591<i>Myxococcus xanthus</i> Encapsulin as a Promising Platform for Intracellular Protein DeliveryAnna N. Gabashvili0Nelly S. Chmelyuk1Viktoria A. Sarkisova2Pavel A. Melnikov3Alevtina S. Semkina4Aleksey A. Nikitin5Maxim A. Abakumov6Laboratory “Biomedical Nanomaterials”, National University of Science and Technology “MISiS”, Leninskiy Avenue, 4, 119049 Moscow, RussiaLaboratory “Biomedical Nanomaterials”, National University of Science and Technology “MISiS”, Leninskiy Avenue, 4, 119049 Moscow, RussiaBiology Faculty, Lomonosov Moscow State University, Leninskiy Gory, 119234 Moscow, RussiaDepartment of Basic and Applied Neurobiology, Serbsky National Medical Research Center for Psychiatry and Narcology, Kropotkinskiy Lane, 23, 119991 Moscow, RussiaDepartment of Medical Nanobiotechnology, Pirogov Russian National Research Medical University, Ostrovityanova Street, 1, 117997 Moscow, RussiaLaboratory “Biomedical Nanomaterials”, National University of Science and Technology “MISiS”, Leninskiy Avenue, 4, 119049 Moscow, RussiaLaboratory “Biomedical Nanomaterials”, National University of Science and Technology “MISiS”, Leninskiy Avenue, 4, 119049 Moscow, RussiaIntroducing a new genetically encoded material containing a photoactivatable label as a model cargo protein, based on <i>Myxococcus xanthus</i> (<i>Mx</i>) encapsulin system stably expressed in human 293T cells. Encapsulin from <i>Mx</i> is known to be a protein-based container for a ferritin-like cargo in its shell which could be replaced with an exogenous cargo protein, resulting in a modified encapsulin system. We replaced <i>Mx</i> natural cargo with a foreign photoactivatable mCherry (PAmCherry) fluorescent protein and isolated encapsulins, containing PAmCherry, from 293T cells. Isolated <i>Mx</i> encapsulin shells containing photoactivatable label can be internalized by macrophages, wherein the PAmCherry fluorescent signal remains clearly visible. We believe that a genetically encoded nanocarrier system obtained in this study, can be used as a platform for controllable delivery of protein/peptide therapeutics in vitro.https://www.mdpi.com/1422-0067/23/24/15591encapsulinsfluorescencephotoactivatable labelprotein delivery |
| spellingShingle | Anna N. Gabashvili Nelly S. Chmelyuk Viktoria A. Sarkisova Pavel A. Melnikov Alevtina S. Semkina Aleksey A. Nikitin Maxim A. Abakumov <i>Myxococcus xanthus</i> Encapsulin as a Promising Platform for Intracellular Protein Delivery International Journal of Molecular Sciences encapsulins fluorescence photoactivatable label protein delivery |
| title | <i>Myxococcus xanthus</i> Encapsulin as a Promising Platform for Intracellular Protein Delivery |
| title_full | <i>Myxococcus xanthus</i> Encapsulin as a Promising Platform for Intracellular Protein Delivery |
| title_fullStr | <i>Myxococcus xanthus</i> Encapsulin as a Promising Platform for Intracellular Protein Delivery |
| title_full_unstemmed | <i>Myxococcus xanthus</i> Encapsulin as a Promising Platform for Intracellular Protein Delivery |
| title_short | <i>Myxococcus xanthus</i> Encapsulin as a Promising Platform for Intracellular Protein Delivery |
| title_sort | i myxococcus xanthus i encapsulin as a promising platform for intracellular protein delivery |
| topic | encapsulins fluorescence photoactivatable label protein delivery |
| url | https://www.mdpi.com/1422-0067/23/24/15591 |
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