Effects of N-cadherin silencing on the proliferation and migration of human dental pulp stem cells
Objective To investigate the effects of N-cadherin silencing on the proliferation and migration of human dental pulp stem cells (DPSCs) and to provide experimental evidence for DPSCs-based dental pulp regeneration. Methods DPSCs were transfected with N-cadherin shRNA lentivirus, and the knockdown ef...
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| Format: | Article |
| Language: | zho |
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Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases
2022-11-01
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| Series: | 口腔疾病防治 |
| Subjects: | |
| Online Access: | http://www.kqjbfz.com/CN/10.12016/j.issn.2096-1456.2022.11.003 |
| _version_ | 1818507868711682048 |
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| author | DENG Zilong YAN Wenjuan ZHAO Wanghong WU Buling |
| author_facet | DENG Zilong YAN Wenjuan ZHAO Wanghong WU Buling |
| author_sort | DENG Zilong |
| collection | DOAJ |
| description | Objective To investigate the effects of N-cadherin silencing on the proliferation and migration of human dental pulp stem cells (DPSCs) and to provide experimental evidence for DPSCs-based dental pulp regeneration. Methods DPSCs were transfected with N-cadherin shRNA lentivirus, and the knockdown efficiency of N-cadherin at both the mRNA and protein levels was confirmed by qRT-PCR and Western blot. The experiment included a negative control group (shRNA -NC) and an N-cadherin shRNA silencing group. Cell proliferation was detected by the CCK-8 method. Cell cycle and apoptosis were assessed by flow cytometry, and cell migration was detected using the Transwell method. Results N-cadherin shRNA significantly reduced the expression levels of N-cadherin mRNA and protein in DPSCs (P<0.001). The proliferation activity of the N-cadherin shRNA group was significantly greater than that of the shRNA-NC group on the 3rd and 4th days after cell inoculation and lower than that of the shRNA-NC group from the 6th to 8th days (P<0.05). On the 3rd day after cell inoculation, the proportion of cells in S phase and G2 phase in the N-cadherin shRNA group was greater than that in the shRNA-NC group (P<0.05). On the 6th day after cell inoculation, the proportion of cells in S phase and G2 phase in the N-cadherin shRNA group was lower than that in the shRNA-NC group (P<0.05), and the proportion of apoptotic cells in the N-cadherin shRNA group was greater than that in the shRNA-NC group (P<0.01). Low densities cells and high densities cells were inoculated into Transwell upper chamber for 20 h, the number of cells passing through the membrane pores of upper chamber in the N-cadherin shRNA group was greater than that in the shRNA-NC group (P<0.001). Conclusion Silencing N-cadherin expression can promote the early proliferation and migration of DPSCs. |
| first_indexed | 2024-12-10T22:23:59Z |
| format | Article |
| id | doaj.art-42ea3bc4a68443959b6f6c610b4c0cba |
| institution | Directory Open Access Journal |
| issn | 2096-1456 |
| language | zho |
| last_indexed | 2024-12-10T22:23:59Z |
| publishDate | 2022-11-01 |
| publisher | Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases |
| record_format | Article |
| series | 口腔疾病防治 |
| spelling | doaj.art-42ea3bc4a68443959b6f6c610b4c0cba2022-12-22T01:31:14ZzhoEditorial Department of Journal of Prevention and Treatment for Stomatological Diseases口腔疾病防治2096-14562022-11-01301177978410.12016/j.issn.2096-1456.2022.11.003Effects of N-cadherin silencing on the proliferation and migration of human dental pulp stem cellsDENG Zilong0YAN Wenjuan1ZHAO Wanghong2WU Buling31. Department of Stomatology, Nanfang Hospital, Southern Medical University 2. College of Stomatology, Southern Medical University1 Department of Stomatology, Nanfang Hospital, Southern Medical University 2. College of Stomatology, Southern Medical University1. Department of Stomatology, Nanfang Hospital, Southern Medical University 2. College of Stomatology, Southern Medical University1. College of Stomatology, Southern Medical University 2.Department of Operative Dentistry and Endodontics, Shenzhen Stomatology Hospital (Pingshan), Southern Medical UniversityObjective To investigate the effects of N-cadherin silencing on the proliferation and migration of human dental pulp stem cells (DPSCs) and to provide experimental evidence for DPSCs-based dental pulp regeneration. Methods DPSCs were transfected with N-cadherin shRNA lentivirus, and the knockdown efficiency of N-cadherin at both the mRNA and protein levels was confirmed by qRT-PCR and Western blot. The experiment included a negative control group (shRNA -NC) and an N-cadherin shRNA silencing group. Cell proliferation was detected by the CCK-8 method. Cell cycle and apoptosis were assessed by flow cytometry, and cell migration was detected using the Transwell method. Results N-cadherin shRNA significantly reduced the expression levels of N-cadherin mRNA and protein in DPSCs (P<0.001). The proliferation activity of the N-cadherin shRNA group was significantly greater than that of the shRNA-NC group on the 3rd and 4th days after cell inoculation and lower than that of the shRNA-NC group from the 6th to 8th days (P<0.05). On the 3rd day after cell inoculation, the proportion of cells in S phase and G2 phase in the N-cadherin shRNA group was greater than that in the shRNA-NC group (P<0.05). On the 6th day after cell inoculation, the proportion of cells in S phase and G2 phase in the N-cadherin shRNA group was lower than that in the shRNA-NC group (P<0.05), and the proportion of apoptotic cells in the N-cadherin shRNA group was greater than that in the shRNA-NC group (P<0.01). Low densities cells and high densities cells were inoculated into Transwell upper chamber for 20 h, the number of cells passing through the membrane pores of upper chamber in the N-cadherin shRNA group was greater than that in the shRNA-NC group (P<0.001). Conclusion Silencing N-cadherin expression can promote the early proliferation and migration of DPSCs.http://www.kqjbfz.com/CN/10.12016/j.issn.2096-1456.2022.11.003dental pulp stem cells，proliferation，migration，n-cadherin，dental pulp regeneration，cell cycle，cell apoptosis，lentivirus，microenvironment， |
| spellingShingle | DENG Zilong YAN Wenjuan ZHAO Wanghong WU Buling Effects of N-cadherin silencing on the proliferation and migration of human dental pulp stem cells 口腔疾病防治 dental pulp stem cells， proliferation， migration， n-cadherin， dental pulp regeneration， cell cycle， cell apoptosis， lentivirus， microenvironment， |
| title | Effects of N-cadherin silencing on the proliferation and migration of human dental pulp stem cells |
| title_full | Effects of N-cadherin silencing on the proliferation and migration of human dental pulp stem cells |
| title_fullStr | Effects of N-cadherin silencing on the proliferation and migration of human dental pulp stem cells |
| title_full_unstemmed | Effects of N-cadherin silencing on the proliferation and migration of human dental pulp stem cells |
| title_short | Effects of N-cadherin silencing on the proliferation and migration of human dental pulp stem cells |
| title_sort | effects of n cadherin silencing on the proliferation and migration of human dental pulp stem cells |
| topic | dental pulp stem cells， proliferation， migration， n-cadherin， dental pulp regeneration， cell cycle， cell apoptosis， lentivirus， microenvironment， |
| url | http://www.kqjbfz.com/CN/10.12016/j.issn.2096-1456.2022.11.003 |
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