Biofilm Formation by Pseudallescheria/Scedosporium Species: A Comparative Study
Pseudallescheria/Scedosporium species are medically important fungi that are present in soil and human impacted areas and capable of causing a wide spectrum of diseases in humans. Although little is known about their pathogenesis, their growth process and infection routes are very similar to those o...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01568/full |
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| author | Rodrigo Rollin-Pinheiro Jardel V. de Meirelles Taissa V. M. Vila Beatriz B. Fonseca Vinicius Alves Susana Frases Sonia Rozental Eliana Barreto-Bergter |
| author_facet | Rodrigo Rollin-Pinheiro Jardel V. de Meirelles Taissa V. M. Vila Beatriz B. Fonseca Vinicius Alves Susana Frases Sonia Rozental Eliana Barreto-Bergter |
| author_sort | Rodrigo Rollin-Pinheiro |
| collection | DOAJ |
| description | Pseudallescheria/Scedosporium species are medically important fungi that are present in soil and human impacted areas and capable of causing a wide spectrum of diseases in humans. Although little is known about their pathogenesis, their growth process and infection routes are very similar to those of Aspergillus species, which grow as biofilms in invasive infections. All nine strains tested here displayed the ability to grow as biofilms in vitro and to produce a dense network of interconnected hyphae on both polystyrene and the surfaces of central venous catheters, but with different characteristics. Scedosporium boydii and S. aurantiacum clinical isolates were able to form biofilms faster than the corresponding environmental strains, as evidenced in kinetic assays for S. boydii and CLSM for S. aurantiacum. Biofilms formed by Pseudallescheria/Scedosporium species had significantly higher resistance to the class of antifungal azole than was observed in planktonic cells, indicating a protective role for this structure. In addition, the clinical S. aurantiacum isolate that formed the most robust biofilms was also more virulent in a larvae Galleria mellonella infection model, suggesting that the ability to form biofilms enhances virulence in Pseudallescheria/Scedosporium species. |
| first_indexed | 2024-04-14T05:19:19Z |
| format | Article |
| id | doaj.art-42f40827ab9f4504b7b89ee1daca3abf |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-14T05:19:19Z |
| publishDate | 2017-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-42f40827ab9f4504b7b89ee1daca3abf2022-12-22T02:10:15ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-08-01810.3389/fmicb.2017.01568290772Biofilm Formation by Pseudallescheria/Scedosporium Species: A Comparative StudyRodrigo Rollin-Pinheiro0Jardel V. de Meirelles1Taissa V. M. Vila2Beatriz B. Fonseca3Vinicius Alves4Susana Frases5Sonia Rozental6Eliana Barreto-Bergter7Laboratório de Química Biológica de Microrganismos, Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de JaneiroRio de Janeiro, BrazilLaboratório de Química Biológica de Microrganismos, Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de JaneiroRio de Janeiro, BrazilLaboratório de Biologia Celular de Fungos, Departamento de Parasitologia e Biologia Celular, Instituto de Biofísica, Universidade Federal do Rio de JaneiroRio de Janeiro, BrazilLaboratório de Biologia Celular de Fungos, Departamento de Parasitologia e Biologia Celular, Instituto de Biofísica, Universidade Federal do Rio de JaneiroRio de Janeiro, BrazilLaboratório de Ultraestrutura Celular Hertha Meyer, Departamento de Parasitologia e Biologia Celular, Instituto de Biofísica, Universidade Federal do Rio de JaneiroRio de Janeiro, BrazilLaboratório de Ultraestrutura Celular Hertha Meyer, Departamento de Parasitologia e Biologia Celular, Instituto de Biofísica, Universidade Federal do Rio de JaneiroRio de Janeiro, BrazilLaboratório de Biologia Celular de Fungos, Departamento de Parasitologia e Biologia Celular, Instituto de Biofísica, Universidade Federal do Rio de JaneiroRio de Janeiro, BrazilLaboratório de Química Biológica de Microrganismos, Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de JaneiroRio de Janeiro, BrazilPseudallescheria/Scedosporium species are medically important fungi that are present in soil and human impacted areas and capable of causing a wide spectrum of diseases in humans. Although little is known about their pathogenesis, their growth process and infection routes are very similar to those of Aspergillus species, which grow as biofilms in invasive infections. All nine strains tested here displayed the ability to grow as biofilms in vitro and to produce a dense network of interconnected hyphae on both polystyrene and the surfaces of central venous catheters, but with different characteristics. Scedosporium boydii and S. aurantiacum clinical isolates were able to form biofilms faster than the corresponding environmental strains, as evidenced in kinetic assays for S. boydii and CLSM for S. aurantiacum. Biofilms formed by Pseudallescheria/Scedosporium species had significantly higher resistance to the class of antifungal azole than was observed in planktonic cells, indicating a protective role for this structure. In addition, the clinical S. aurantiacum isolate that formed the most robust biofilms was also more virulent in a larvae Galleria mellonella infection model, suggesting that the ability to form biofilms enhances virulence in Pseudallescheria/Scedosporium species.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01568/fullfungal biofilmsScedosporiumPseudallescheriavirulenceantifungal susceptibility |
| spellingShingle | Rodrigo Rollin-Pinheiro Jardel V. de Meirelles Taissa V. M. Vila Beatriz B. Fonseca Vinicius Alves Susana Frases Sonia Rozental Eliana Barreto-Bergter Biofilm Formation by Pseudallescheria/Scedosporium Species: A Comparative Study Frontiers in Microbiology fungal biofilms Scedosporium Pseudallescheria virulence antifungal susceptibility |
| title | Biofilm Formation by Pseudallescheria/Scedosporium Species: A Comparative Study |
| title_full | Biofilm Formation by Pseudallescheria/Scedosporium Species: A Comparative Study |
| title_fullStr | Biofilm Formation by Pseudallescheria/Scedosporium Species: A Comparative Study |
| title_full_unstemmed | Biofilm Formation by Pseudallescheria/Scedosporium Species: A Comparative Study |
| title_short | Biofilm Formation by Pseudallescheria/Scedosporium Species: A Comparative Study |
| title_sort | biofilm formation by pseudallescheria scedosporium species a comparative study |
| topic | fungal biofilms Scedosporium Pseudallescheria virulence antifungal susceptibility |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01568/full |
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