Purple corn extract alleviates 2,4-dinitrochlorobenzene-induced atopic dermatitis-like phenotypes in BALB/c mice
Zea mays L. (Poaceae), also known as purple corn, is an annual herbaceous plant that is grown as food for human consumption in a variety of forms, including cooking oils and sweeteners in processed food and beverage products. The purpose of this study was to determine whether a novel purple corn ext...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2021-09-01
|
| Series: | Animal Cells and Systems |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/19768354.2021.1974938 |
| _version_ | 1818751940698308608 |
|---|---|
| author | Huiwon No Sang Hyun Nam Hee Won Seo JeongHyeon Seo Soo-Hyun Park Set-Byeol Kim Jun-Sub Jung Jongyeol Park Jaekeun Choi Jae-Yong Lee Hong-Won Suh Soon Sung Lim Jin-Kyung Kim |
| author_facet | Huiwon No Sang Hyun Nam Hee Won Seo JeongHyeon Seo Soo-Hyun Park Set-Byeol Kim Jun-Sub Jung Jongyeol Park Jaekeun Choi Jae-Yong Lee Hong-Won Suh Soon Sung Lim Jin-Kyung Kim |
| author_sort | Huiwon No |
| collection | DOAJ |
| description | Zea mays L. (Poaceae), also known as purple corn, is an annual herbaceous plant that is grown as food for human consumption in a variety of forms, including cooking oils and sweeteners in processed food and beverage products. The purpose of this study was to determine whether a novel purple corn extract, FB801, might have an anti-atopic dermatitis (AD) effect on AD-like skin lesions induced by 2,4-dinitrochlorobenzene (DNCB) in BALB/c mice. Topical sensitization (1%) and challenge (0.3%) by DNCB were performed on the dorsal skin and right ear of BALB/c mice to induce AD. Following FB801 and dexamethasone administered orally, the severity of skin lesions was examined macroscopically and histologically. Serum levels of immunoglobulin E (IgE) and various cytokines were determined by enzyme-linked immunosorbent assay. Oral administration of FB801 significantly reduced typical symptoms of AD (erythema/bleeding, swelling, molting/erosion and scaling/drying), scratching frequencies, and the recruitment of inflammatory and mast cells. In addition, FB801 suppressed serum levels of IgE and T helper (Th)2 type cytokines such as interleukin (IL)-4 and IL-10 in DNCB-treated BALB/c mice. Furthermore, FB801 reduced the degradation of inhibitor of nuclear factor-κB proteins (NF-κB) in tumor necrosis factor (TNF)-α-stimulated human keratinocyte (HaCaT) cells. These results suggest that FB801 inhibited the development of the AD-like skin symptoms by regulating Th1 and Th2 responses in the skin lesions in mice and suppressing TNF-α induced NF-κB activation in HaCaT cells, suggesting that FB801 has potential application as an effective alternative therapy for the prevention and management of AD. |
| first_indexed | 2024-12-18T04:43:33Z |
| format | Article |
| id | doaj.art-42f43ff4dba646da9d37fc3f381df544 |
| institution | Directory Open Access Journal |
| issn | 1976-8354 2151-2485 |
| language | English |
| last_indexed | 2024-12-18T04:43:33Z |
| publishDate | 2021-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj.art-42f43ff4dba646da9d37fc3f381df5442022-12-21T21:20:38ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852021-09-0125527228210.1080/19768354.2021.19749381974938Purple corn extract alleviates 2,4-dinitrochlorobenzene-induced atopic dermatitis-like phenotypes in BALB/c miceHuiwon No0Sang Hyun Nam1Hee Won Seo2JeongHyeon Seo3Soo-Hyun Park4Set-Byeol Kim5Jun-Sub Jung6Jongyeol Park7Jaekeun Choi8Jae-Yong Lee9Hong-Won Suh10Soon Sung Lim11Jin-Kyung Kim12Daegu Catholic UniversityDaegu Catholic UniversityDaegu Catholic UniversityDaegu Catholic UniversityFront Bio Inc.Front Bio Inc.Front Bio Inc.Maize Research InstituteMaize Research InstituteFront Bio Inc.Front Bio Inc.Front Bio Inc.Daegu Catholic UniversityZea mays L. (Poaceae), also known as purple corn, is an annual herbaceous plant that is grown as food for human consumption in a variety of forms, including cooking oils and sweeteners in processed food and beverage products. The purpose of this study was to determine whether a novel purple corn extract, FB801, might have an anti-atopic dermatitis (AD) effect on AD-like skin lesions induced by 2,4-dinitrochlorobenzene (DNCB) in BALB/c mice. Topical sensitization (1%) and challenge (0.3%) by DNCB were performed on the dorsal skin and right ear of BALB/c mice to induce AD. Following FB801 and dexamethasone administered orally, the severity of skin lesions was examined macroscopically and histologically. Serum levels of immunoglobulin E (IgE) and various cytokines were determined by enzyme-linked immunosorbent assay. Oral administration of FB801 significantly reduced typical symptoms of AD (erythema/bleeding, swelling, molting/erosion and scaling/drying), scratching frequencies, and the recruitment of inflammatory and mast cells. In addition, FB801 suppressed serum levels of IgE and T helper (Th)2 type cytokines such as interleukin (IL)-4 and IL-10 in DNCB-treated BALB/c mice. Furthermore, FB801 reduced the degradation of inhibitor of nuclear factor-κB proteins (NF-κB) in tumor necrosis factor (TNF)-α-stimulated human keratinocyte (HaCaT) cells. These results suggest that FB801 inhibited the development of the AD-like skin symptoms by regulating Th1 and Th2 responses in the skin lesions in mice and suppressing TNF-α induced NF-κB activation in HaCaT cells, suggesting that FB801 has potential application as an effective alternative therapy for the prevention and management of AD.http://dx.doi.org/10.1080/19768354.2021.1974938atopic dermatitis2,4-dinitrochlorobenzenepurple cornige |
| spellingShingle | Huiwon No Sang Hyun Nam Hee Won Seo JeongHyeon Seo Soo-Hyun Park Set-Byeol Kim Jun-Sub Jung Jongyeol Park Jaekeun Choi Jae-Yong Lee Hong-Won Suh Soon Sung Lim Jin-Kyung Kim Purple corn extract alleviates 2,4-dinitrochlorobenzene-induced atopic dermatitis-like phenotypes in BALB/c mice Animal Cells and Systems atopic dermatitis 2,4-dinitrochlorobenzene purple corn ige |
| title | Purple corn extract alleviates 2,4-dinitrochlorobenzene-induced atopic dermatitis-like phenotypes in BALB/c mice |
| title_full | Purple corn extract alleviates 2,4-dinitrochlorobenzene-induced atopic dermatitis-like phenotypes in BALB/c mice |
| title_fullStr | Purple corn extract alleviates 2,4-dinitrochlorobenzene-induced atopic dermatitis-like phenotypes in BALB/c mice |
| title_full_unstemmed | Purple corn extract alleviates 2,4-dinitrochlorobenzene-induced atopic dermatitis-like phenotypes in BALB/c mice |
| title_short | Purple corn extract alleviates 2,4-dinitrochlorobenzene-induced atopic dermatitis-like phenotypes in BALB/c mice |
| title_sort | purple corn extract alleviates 2 4 dinitrochlorobenzene induced atopic dermatitis like phenotypes in balb c mice |
| topic | atopic dermatitis 2,4-dinitrochlorobenzene purple corn ige |
| url | http://dx.doi.org/10.1080/19768354.2021.1974938 |
| work_keys_str_mv | AT huiwonno purplecornextractalleviates24dinitrochlorobenzeneinducedatopicdermatitislikephenotypesinbalbcmice AT sanghyunnam purplecornextractalleviates24dinitrochlorobenzeneinducedatopicdermatitislikephenotypesinbalbcmice AT heewonseo purplecornextractalleviates24dinitrochlorobenzeneinducedatopicdermatitislikephenotypesinbalbcmice AT jeonghyeonseo purplecornextractalleviates24dinitrochlorobenzeneinducedatopicdermatitislikephenotypesinbalbcmice AT soohyunpark purplecornextractalleviates24dinitrochlorobenzeneinducedatopicdermatitislikephenotypesinbalbcmice AT setbyeolkim purplecornextractalleviates24dinitrochlorobenzeneinducedatopicdermatitislikephenotypesinbalbcmice AT junsubjung purplecornextractalleviates24dinitrochlorobenzeneinducedatopicdermatitislikephenotypesinbalbcmice AT jongyeolpark purplecornextractalleviates24dinitrochlorobenzeneinducedatopicdermatitislikephenotypesinbalbcmice AT jaekeunchoi purplecornextractalleviates24dinitrochlorobenzeneinducedatopicdermatitislikephenotypesinbalbcmice AT jaeyonglee purplecornextractalleviates24dinitrochlorobenzeneinducedatopicdermatitislikephenotypesinbalbcmice AT hongwonsuh purplecornextractalleviates24dinitrochlorobenzeneinducedatopicdermatitislikephenotypesinbalbcmice AT soonsunglim purplecornextractalleviates24dinitrochlorobenzeneinducedatopicdermatitislikephenotypesinbalbcmice AT jinkyungkim purplecornextractalleviates24dinitrochlorobenzeneinducedatopicdermatitislikephenotypesinbalbcmice |