BRAF gene mutation (V600E) in aspiration cytology of patients with suspected papillary thyroid carcinoma
Abstract Introduction: This study was attempted to investigate the prevalence of BRAF gene mutation (V600E) in aspiration cytology of patients with suspected papillary thyroid carcinoma (PTC). Materials and methods: Seventy six Patients suspected of having PTC who referred for fine needle...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tehran University of Medical Sciences
2021-10-01
|
| Series: | Acta Medica Iranica |
| Subjects: | |
| Online Access: | https://acta.tums.ac.ir/index.php/acta/article/view/8828 |
| _version_ | 1818775711773622272 |
|---|---|
| author | Mohammadhossein Dadgarnia Mohsen Abouii Mohammadhossein Baradaranfar Sedighe Vaziribozorg Vahid Zand |
| author_facet | Mohammadhossein Dadgarnia Mohsen Abouii Mohammadhossein Baradaranfar Sedighe Vaziribozorg Vahid Zand |
| author_sort | Mohammadhossein Dadgarnia |
| collection | DOAJ |
| description | Abstract
Introduction: This study was attempted to investigate the prevalence of BRAF gene mutation (V600E) in aspiration cytology of patients with suspected papillary thyroid carcinoma (PTC).
Materials and methods: Seventy six Patients suspected of having PTC who referred for fine needle aspiration (FNA) biopsy were included in this cross-sectional study. Ultrasound-guided FNA was taken from the thyroid masses and samples were sent for cytologic evaluation. Simultaneously, the samples were sent to a genetic laboratory to check the status of BRAFV600E mutation. Patients with FNA positive for PTC were assigned in one group and those with FNA negative for PTC were assigned in other group. Cytological and molecular results were compared with those of histopathology and sonography.
Results: The results showed that the prevalence of the BRAF gene (V600E) mutation in our study was 21.1% (16 out of 76 patients). In addition the results showed a significant relation between gene mutation and pathologic findings so that the highest gene mutation was significantly detected in patients with FNA positive for PTC (p.value=0.001). Also, our results showed a significant relation between gene mutation and some sonographic findings (calcification, p.value=0.004) and no significant relation in the other sonographic findings (hypo echoic changes, p.value=1.12 and regular changes, p.value=0.194).
Conclusion: According to the results of the present study, BRAF mutation (V600E) can be an effective indicator for definitive diagnosis and primary treatment of PTC in suspected cases. |
| first_indexed | 2024-12-18T11:01:23Z |
| format | Article |
| id | doaj.art-42f6308a0e874c7cbe056f8b32efb99d |
| institution | Directory Open Access Journal |
| issn | 0044-6025 1735-9694 |
| language | English |
| last_indexed | 2024-12-18T11:01:23Z |
| publishDate | 2021-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Acta Medica Iranica |
| spelling | doaj.art-42f6308a0e874c7cbe056f8b32efb99d2022-12-21T21:10:12ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942021-10-01599BRAF gene mutation (V600E) in aspiration cytology of patients with suspected papillary thyroid carcinomaMohammadhossein Dadgarnia0Mohsen Abouii1Mohammadhossein Baradaranfar2Sedighe Vaziribozorg3Vahid Zand41. Department of Otolaryngology- Head and Neck Surgery, Otorhinolaryngology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, IranDepartment of Otolaryngology-Head and Neck Surgery, Otorhinolaryngology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.1. Department of Otolaryngology- Head and Neck Surgery, Otorhinolaryngology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran1. Department of Otolaryngology- Head and Neck Surgery, Otorhinolaryngology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran1. Department of Otolaryngology- Head and Neck Surgery, Otorhinolaryngology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, IranAbstract Introduction: This study was attempted to investigate the prevalence of BRAF gene mutation (V600E) in aspiration cytology of patients with suspected papillary thyroid carcinoma (PTC). Materials and methods: Seventy six Patients suspected of having PTC who referred for fine needle aspiration (FNA) biopsy were included in this cross-sectional study. Ultrasound-guided FNA was taken from the thyroid masses and samples were sent for cytologic evaluation. Simultaneously, the samples were sent to a genetic laboratory to check the status of BRAFV600E mutation. Patients with FNA positive for PTC were assigned in one group and those with FNA negative for PTC were assigned in other group. Cytological and molecular results were compared with those of histopathology and sonography. Results: The results showed that the prevalence of the BRAF gene (V600E) mutation in our study was 21.1% (16 out of 76 patients). In addition the results showed a significant relation between gene mutation and pathologic findings so that the highest gene mutation was significantly detected in patients with FNA positive for PTC (p.value=0.001). Also, our results showed a significant relation between gene mutation and some sonographic findings (calcification, p.value=0.004) and no significant relation in the other sonographic findings (hypo echoic changes, p.value=1.12 and regular changes, p.value=0.194). Conclusion: According to the results of the present study, BRAF mutation (V600E) can be an effective indicator for definitive diagnosis and primary treatment of PTC in suspected cases.https://acta.tums.ac.ir/index.php/acta/article/view/8828papillary thyroid cancerfine needle aspirationBRAF gene (V600E) mutationultrasound |
| spellingShingle | Mohammadhossein Dadgarnia Mohsen Abouii Mohammadhossein Baradaranfar Sedighe Vaziribozorg Vahid Zand BRAF gene mutation (V600E) in aspiration cytology of patients with suspected papillary thyroid carcinoma Acta Medica Iranica papillary thyroid cancer fine needle aspiration BRAF gene (V600E) mutation ultrasound |
| title | BRAF gene mutation (V600E) in aspiration cytology of patients with suspected papillary thyroid carcinoma |
| title_full | BRAF gene mutation (V600E) in aspiration cytology of patients with suspected papillary thyroid carcinoma |
| title_fullStr | BRAF gene mutation (V600E) in aspiration cytology of patients with suspected papillary thyroid carcinoma |
| title_full_unstemmed | BRAF gene mutation (V600E) in aspiration cytology of patients with suspected papillary thyroid carcinoma |
| title_short | BRAF gene mutation (V600E) in aspiration cytology of patients with suspected papillary thyroid carcinoma |
| title_sort | braf gene mutation v600e in aspiration cytology of patients with suspected papillary thyroid carcinoma |
| topic | papillary thyroid cancer fine needle aspiration BRAF gene (V600E) mutation ultrasound |
| url | https://acta.tums.ac.ir/index.php/acta/article/view/8828 |
| work_keys_str_mv | AT mohammadhosseindadgarnia brafgenemutationv600einaspirationcytologyofpatientswithsuspectedpapillarythyroidcarcinoma AT mohsenabouii brafgenemutationv600einaspirationcytologyofpatientswithsuspectedpapillarythyroidcarcinoma AT mohammadhosseinbaradaranfar brafgenemutationv600einaspirationcytologyofpatientswithsuspectedpapillarythyroidcarcinoma AT sedighevaziribozorg brafgenemutationv600einaspirationcytologyofpatientswithsuspectedpapillarythyroidcarcinoma AT vahidzand brafgenemutationv600einaspirationcytologyofpatientswithsuspectedpapillarythyroidcarcinoma |